Multidrug-resistant Salmonella spp. in fecal samples of pigs with suspected salmonellosis in Antioquia, Colombia, 2019–2021

Vidal, Juana L.; Clavijo, Viviana; Castellanos, Luis R.; Jeyashree, Kathiresan; Kumar, Ajay M. V.; Mehta, Kedar; Chaparro-Gutiérrez, Jenny J.

doi:10.26633/rpsp.2023.46

Cited by 5 publications

(1 citation statement)

References 22 publications

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“…Recently, Salmonella serovars were responsible for multiple outbreaks linked to alfalfa sprouts, cantaloupe, seafood, and peanut butter (5). Given the global emergence of multidrug-resistant Salmonella strains (6)(7)(8) there is an urgency to develop alternatives to traditional antibiotic therapy, which could leverage understanding of the metabolic targets that sustain pathogen infection.…”

Section: Introductionmentioning

confidence: 99%

Gut microbiome carbon and sulfur metabolisms supportSalmonelladuring pathogen infection

Leleiwi,

Kokkinias,

Kim

et al. 2024

Preprint

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Salmonella enterica serovar Typhimurium is a pervasive enteric pathogen and an ongoing global threat to public health. Ecological studies in the Salmonella impacted gut remain underrepresented in the literature, discounting the microbiome mediated interactions that may inform Salmonella physiology during colonization and infection. To understand the microbial ecology of Salmonella remodeling of the gut microbiome, here we performed multi-omics approaches on fecal microbial communities from untreated and Salmonella-infected mice. Reconstructed genomes recruited metatranscriptomic and metabolomic data providing a strain-resolved view of the expressed metabolisms of the microbiome during Salmonella infection. This data informed possible Salmonella interactions with members of the gut microbiome that were previously uncharacterized. Salmonella-induced inflammation significantly reduced the diversity of transcriptionally active members in the gut microbiome, yet increased gene expression was detected for 7 members, with Luxibacter and Ligilactobacillus being the most active. Metatranscriptomic insights from Salmonella and other persistent taxa in the inflamed microbiome further expounded the necessity for oxidative tolerance mechanisms to endure the host inflammatory responses to infection. In the inflamed gut lactate was a key metabolite, with microbiota production and consumption reported amongst transcriptionally active members. We also showed that organic sulfur sources could be converted by gut microbiota to yield inorganic sulfur pools that become oxidized in the inflamed gut, resulting in thiosulfate and tetrathionate that supports Salmonella respiration. Advancement of pathobiome understanding beyond inferences from prior amplicon-based approaches can hold promise for infection mitigation, with the active community outlined here offering intriguing organismal and metabolic therapeutic targets.

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Section: Introductionmentioning

confidence: 99%

Gut microbiome carbon and sulfur metabolisms supportSalmonelladuring pathogen infection

Leleiwi,

Kokkinias,

Kim

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Gut microbiota carbon and sulfur metabolisms support Salmonella infections

Leleiwi,

Kokkinias,

Kim

et al. 2024

The ISME Journal

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Salmonella enterica serovar Typhimurium is a pervasive enteric pathogen and ongoing global threat to public health. Ecological studies in the Salmonella impacted gut remain underrepresented in the literature, discounting microbiome mediated interactions that may inform Salmonella physiology during colonization and infection. To understand the microbial ecology of Salmonella remodeling of the gut microbiome, we performed multi-omics on fecal microbial communities from untreated and Salmonella-infected mice. Reconstructed genomes recruited metatranscriptomic and metabolomic data providing a strain-resolved view of the expressed metabolisms of the microbiome during Salmonella infection. These data informed possible Salmonella interactions with members of the gut microbiome that were previously uncharacterized. Salmonella-induced inflammation significantly reduced the diversity of genomes that recruited transcripts in the gut microbiome, yet increased transcript mapping was observed for 7 members, among which Luxibacter and Ligilactobacillus transcript read recruitment was most prevalent. Metatranscriptomic insights from Salmonella and other persistent taxa in the inflamed microbiome further expounded the necessity for oxidative tolerance mechanisms to endure the host inflammatory responses to infection. In the inflamed gut lactate was a key metabolite, with microbiota production and consumption reported amongst members with detected transcript recruitment. We also showed that organic sulfur sources could be converted by gut microbiota to yield inorganic sulfur pools that become oxidized in the inflamed gut, resulting in thiosulfate and tetrathionate that supports Salmonella respiration. This research advances physiological microbiome insights beyond prior amplicon-based approaches, with the transcriptionally active organismal and metabolic pathways outlined here offering intriguing intervention targets in the Salmonella-infected intestine.

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Assessing antimicrobial resistance profiles of Salmonella enterica in the pork production system

Prasertsee,

Prachantasena,

Tantitaveewattana

et al. 2024

Journal of Medical Microbiology

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Introduction. Salmonella enterica is a significant enteric pathogen affecting human and livestock health. Pork production is a common source of Salmonella contamination, with emerging multidrug resistance (MDR) posing a global health threat. Gap statement. Salmonella contamination and antimicrobial resistance (AMR) profiles in the pig production chain are underreported. Aim. To investigate the prevalence of S. enterica in the pig production chain and characterise their AMR profiles. Methodology. We collected 485 samples from pig farms, a standard pig abattoir and retail markets in Patthalung and Songkhla provinces in southern Thailand. Antimicrobial susceptibility testing was performed on these samples, and AMR profiles were determined. Results. S. enterica was detected in 68.67% of farm samples, 45.95% of abattoir samples and 50.67% of retail market samples. Analysis of 264 isolates, representing 18 serotypes, identified S. enterica serotype Rissen as the most prevalent. The predominant resistance phenotypes included ampicillin (AMP, 91.29%), tetracycline (TET, 88.26%) and streptomycin (STR, 84.47%). Over 80% of isolates showed resistance to three or more antimicrobial classes, indicating MDR. The AMP–STR–TET resistance pattern was found in nearly 70% of all MDR isolates across the production chain. Conclusions. The high prevalence of MDR is consistent with extensive antimicrobial use in the livestock sector. The presence of extensively resistant S. enterica highlights the urgent need for antimicrobial stewardship. Strengthening preventive strategies and control measures is crucial to mitigate the risk of MDR Salmonella spreading from farm to fork.

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Multidrug-resistant Salmonella spp. in fecal samples of pigs with suspected salmonellosis in Antioquia, Colombia, 2019–2021

Cited by 5 publications

References 22 publications

Gut microbiome carbon and sulfur metabolisms supportSalmonelladuring pathogen infection

Gut microbiome carbon and sulfur metabolisms supportSalmonelladuring pathogen infection

Gut microbiota carbon and sulfur metabolisms support Salmonella infections

Assessing antimicrobial resistance profiles of Salmonella enterica in the pork production system

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