Multidrug-resistant  Vibrio  associated with an estuary affected by shrimp farming in Northeastern Brazil

Rocha, Rafael dos Santos; Sousa, Oscarina Viana de; Vieira, Regine Helena Silva dos Fernandes

doi:10.1016/j.marpolbul.2016.02.001

Cited by 27 publications

(25 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, due to the expansion of fish production worldwide, fish waste can impact the local environment by polluting the water and soil, considered one of the major worries of this practice. Therefore, the developments of efficient water reuse systems that reduce the environmental impacts and enable sustainable growth are considered necessary for this activity (Rocha et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Enterococcus spp. isolated from a fish farming environment in southern Brazil

Araújo¹,

Grassotti²,

Frazzon³

2021

Braz. J. Biol.

View full text Add to dashboard Cite

The aim of present study is to characterize the resistance and virulence profile of enterococci isolated from aquaculture excavated ponds and masonry tanks (6 samples) in southern Brazil. Samples were cultured in selective medium, 10 colonies were randomly selected from each sample, which were identified by MALDI-TOF and tested against 13 antimicrobials. The presence of resistance (tetL, tetM, tetS, ermB and msrC) and virulence (ace, esp, agg, cylA and gelE) genes were determined by PCR. A total of 79 enterococci were identified, and Entecococcus faecalis (44.3%) and E. casseliflavus (36.7%) were the most prevalent species isolated. Sixty-five strains (82.3%) were resistant to at least one of the antimicrobials tested, whereas 27 (34.2%) strains were multiresistant. The overall percentages of antimicrobial resistant isolates were: 58.2% to rifampicin, 40.5% to fluoroquinolones, 36.7% to erythromycin and 30.4% to tetracycline. The tetL and tetM genes were found in 57.7% of the tetracycline-resistant strains; and msrC in 31.01% of erythromycin-resistant strains. The most frequently detected virulence factors were ace and gelE genes. Although limited to a single farm, these data suggest that aquaculture may be a reservoir of resistant and virulent enterococci. This study is the first step towards enhancing our understandingof distribution, resistance and virulence profile in enterococci isolated from fish farming environments in the south Brazil.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterization of Enterococcus spp. isolated from a fish farming environment in southern Brazil

Araújo¹,

Grassotti²,

Frazzon³

2021

Braz. J. Biol.

View full text Add to dashboard Cite

show abstract

“…The widespread use of antibiotics to control diseases in aquaculture not only increases the pressure on the microbial community but also promotes the emergence of bacterial resistance (Stalin & Srinivasan, ). Bacteria can acquire resistance through the transfer of mobile genetic elements (such as plasmids, phages, integrons and transposons) and horizontal transfer of antibiotic resistant shapes (Rocha & Sousa, ; You, Bong, & Lee, ). Acquired drug resistance may be single‐drug resistant or multidrug resistant (MDR), which occurs when bacteria acquire multiple genetic factors from different sources through different conjugation or transformation.…”

Section: Discussionmentioning

confidence: 99%

Honokiol inhibitsVibrio harveyihemolysin virulence by reducing its haemolytic activity

Guo

Xing

et al. 2019

Aquac Res

View full text Add to dashboard Cite

Vibrio harveyi, as one of the main pathogens of vibriosis, has serious multidrug resistance, which has been a focus for domestic and foreign researchers. Vibrio harveyi hemolysin (VHH), an important virulence determinant of V. harveyi, causes cell necrosis and tissue damage by lysing cell membranes, which is essential for V. harveyi invasion of the host. The critical role of VHH in the virulence of V. harveyi renders it an ideal target for designing novel anti-virulence therapeutics. We found that honokiol, a natural compound, could act as a potent antagonist of VHH-mediated haemolysis at non-bacteriostatic concentrations. Honokiol can effectively inhibit the damage caused by VHH and V. harveyi both in vitro and in vivo. qRT-PCR and molecular docking analysis showed that although honokiol up-regulated the transcription level of vhh, it could block the active centre of VHH directly and forcefully, thereby reducing the catalytic activity of VHH. The results suggest that HNK could have great potential in the development of new aquaculture drugs. K E Y W O R D S anti-virulence, honokiol, VHH, Vibrio harveyi

show abstract

“…Quanto maior a densidade de povoamento dos tanques e viveiros, maior é probabilidade de surtos de doenças e maiores são os controles necessários para manutenção da sobrevivência e do bem estar dos camarões, sendo em muitos casos necessário intervenções profiláticas e terapêuticas com uso de antimicrobianos já desde os estágios de geração de pós-larvas até a engorda, para prevenção ou tratamento de enfermidades causadas principalmente por bactérias, vírus, fungos e protozoários (HOLMSTROM et al, 2003;FLEGEL, 2012;CABELLO et al, 2013;RIBEIRO et al, 2014;ROCHA et al, 2016;TASSANAKAJON et al, 2018;RAMOS, 2018). Além disso, o equilíbrio entre os fatores biológicos e não biológicos, ditam a qualidade do ambiente de cultivo, combatem o estresse e asseguram a manutenção da resposta imunológica dos camarões a enfermidades (CHEN; HE, 2019).…”

Section: Panorama Da Carcinicultura No Brasil E No Mundounclassified

“…O uso de antibióticos para tratamento e prevenção das bacterioses é uma alternativa para o combate às perdas produtivas ocasionadas por doenças. Dentre as substâncias utilizadas destacam-se das classes das fluoroquinolonas, betalactâmicos, sulfonamidas, tetraciclinas e anfenicóis (HOLMSTROM et al, 2003;RIBEIRO et al, 2014;ROCHA et al, 2016 (GAO et al, 2012a;ZHOU-JL et al, 2012;LIANG et al, 2013;SHI et al, 2014;CHEN et al, 2015;CHU, 2016;LI et al, 2018b). A água é, portanto, um vetor de resíduos de antibióticos no ambiente, razão de muitos estudos terem se concentrado em ambientes aquáticos e suas matrizes (THUY et al, 2013;FAO, 2016a;2016b;RAMOS, 2018;LI et al, 2018b).…”

Section: Antibióticos Na Carciniculturaunclassified

“…Litopenaeus Vannamei são os mais cultivados e de relevância econômica ao setor (FAO, 2016b; FAO, 2019) e onde resíduos e cepas de bactérias patogênicas com multirresistência a antibióticos têm sido identificados, inclusive para aqueles com uso já descontinuado, mas ainda presentes, ligados a sedimentos que atrasam sua biodegradação (CARVALHO et al, 2013;COSTA et al, 2015;LAI et al, 2015;ROCHA et al, 2016). Ações imediatistas no combate a surtos de doenças ou mesmo no uso racional merecem enfoque das autoridades (FAO, 2016b;.…”

Section: Introductionunclassified

See 1 more Smart Citation

Ocorrência de antibióticos em fazendas de cultivo de camarão (Litopenaeus vannamei) no estado do Rio Grande do Norte - Brasil

Armelim¹

View full text Add to dashboard Cite

Considering the factors of recognized use of different antibiotics in the global shrimp farming, the productive potential of shrimps in the northeastern Brazilian region, the residue monitoring scopes of inspection agencies and the risks associated with the use of antibiotics for food safety and public health, as well as toxicity and mechanisms of development of pathogenic microbial resistance, especially in the aquatic environment, the aim of the study was to determine, by the liquid chromatography coupled to mass spectrometry with source (LC-ESI-MS/MS), the occurrence of multiclasses and multiresidues of 12 antibiotics belonging to tetracyclines, sulfonamides, fluoroquinolones and amphenicols in shrimp (Litopenaeus vannamei), water and sediments from 5 farms shrimp farms with different models of production, present in the state of Rio Grande do Norte-Brazil. Raised nurseries were selected for collection of the 3 matrices and points upstream and downstream of the nurseries, for the collection of environmental matrices destined to the study of spatial distribution of residues in the farms. Physicalchemical parameters of water quality were evaluated during sampling. On line Solidphase extraction techniques were used to LC-MS/MS for sediment, Captiva cartridges for filtration of shrimp muscle extracts and off-line solid-phase extraction to LC-MS/MS in surface water, with the samples packed in SPE cartridges prior to the transportation to the laboratory.The chromatographic methods validations were done for the parameters of selectivity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, precision and long-term stability of the analytes in the matrix. LOQs ranged from 0.82 (sulfadimethoxine) to 7.63 (chloramphenicol) ng mL-1 for water, 1.23 (tetracycline) at 15.99 (chloramphenicol) μg kg-1 for sediment and 1.24 (enrofloxacin) at 12.05 (sulfatiazole) μg kg-1 for shrimp. Sulfadimethoxine (23.4 ng L-1) and florfenicol (10.78 to 175.77 ng L-1) residues were the only antibiotics detected and quantified in nursery water. In sediments, oxytetracycline, enrofloxacin and norfloxacin residues were detected only upstream of the nurseries and at levels

show abstract

Multidrug-resistant Vibrio associated with an estuary affected by shrimp farming in Northeastern Brazil

Cited by 27 publications

References 31 publications

Characterization of Enterococcus spp. isolated from a fish farming environment in southern Brazil

Characterization of Enterococcus spp. isolated from a fish farming environment in southern Brazil

Honokiol inhibitsVibrio harveyihemolysin virulence by reducing its haemolytic activity

Ocorrência de antibióticos em fazendas de cultivo de camarão (Litopenaeus vannamei) no estado do Rio Grande do Norte - Brasil

Contact Info

Product

Resources

About