Multielement Analysis of Agar and Gelrite Impurities Investigated by Inductively Coupled Plasma Emission Spectrometry as Well as Physical Properties of Tissue Culture Media Prepared With Agar or the Gellan Gum Gelrite

Scherer, Paul; Mueller, E. F.; Lippert, H.; Wolff, G.

doi:10.17660/actahortic.1988.226.91

Cited by 43 publications

(28 citation statements)

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“…Phytagar (7 gl -l) and Gelrite (2 g1-1) contributed approx. 0.67 and 0.06 ppm B, respectively, which agreed with reports on nutrient composition analysis for different gelling agents (Singha & Townsend, 1985;Scherer & Muller, 1988). Therefore, when B effects on plantlets are investigated, the contamination factor should be taken into consideration.…”

Section: Cultivarsupporting

confidence: 86%

“…In this experiment Gelrite was used to solidify the medium, since this material contains only minute amounts of B (Scherer & Muller, 1988), and even less medium B was added (25 or 50% of MS control levels). The objective of this experiment was to evaluate the effect of two medium Ca levels (3 (MS control level) or 9 mM Ca) and low medium B levels ( 0.025, 0.05 or 0.1 (MS control level) mM B) on Ca uptake (% DW) in plantlet organs and on plantlet growth in cvs Bintje and Norland.…”

Section: Methodsmentioning

confidence: 99%

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The effect of boron on calcium uptake and growth in micropropagated potato plantlets

2000

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SummaryBoron (B) requirements differ widely among plant species and the concentration range between toxicity and deficiency is less for B than for any other nutrient. Excess B can adversely affect calcium (Ca) uptake and plant growth. Potato cvs Bintje and Norland plantlets were micropropagated on Murashige & Skoog (MS) nutrient medium, supplemented with 3 (MS control level) or 9 mM Ca, and a range of HsBO3 levels (0.025 to 0.300 mM B). Medium B levels of 0.100 and 0.300 mM decreased Ca cotitentin leaves and shoots of cv. Norlafid, but not Bintje. Medium B level of 0.025 mM, which is 25 % of the control MS level, enhanced Ca uptake in cv. Norland and did not compromise normal plantlet growth in either cultivar. This lower H~B03 level (0.025 mM B), and a B-free gelling agent such as Gelrite, could be used for mlcropropagation of potato and possibly other species susceptible to Ca deficiency disorders.

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Section: Cultivarsupporting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

The effect of boron on calcium uptake and growth in micropropagated potato plantlets

2000

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“…If these components are necessary for the growth of root primordia, then they must be present in sufficient quantities in the buds. Agar and Gelrite, though differing in their composition and mineral impurities (Scherer et al 1988), did not cause differences in rooting. Where possible, Gelrite was used in preference to agar because it is more transparent and allowed easier observation of root emergence.…”

Section: Results Discussionmentioning

confidence: 85%

Promotion of root emergence in vitro from rhizome buds of Lapageria rosea cv. Nashcourt after proliferation in the presence of paclobutrazol

McKinless

Alderson

1993

Plant Cell Tiss Organ Cult

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Medium components that may influence the emergence of adventitious root primordia in rhizome buds of Lapageria rosea (Ruiz et Pav.) cv. Nashcourt in vitro were examined. Sucrose was the only medium component tested that affected root emergence. Increasing the sucrose concentration in the medium from 10 to 30g 1-1 stimulated adventitious root emergence from rhizome buds that had been proliferated on a medium containing the gibberellin biosynthesis inhibitor paclobutrazol (5 txM).Successful clonal propagation of L. rosea cv. Nashcourt can now be achieved by a rhizome bud proliferation stage(s) in the presence of paclobutrazol followed by an adventitious root emergence stage in the absence of paclobutrazol.

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“…Agar contains a large amount of sodium and also levels of sulfur and copper are significant. Gelrite has less organic impurities but inorganic ones exist at high concentrations 23,24 . The addition of 4 g l −1 gelrite as the gelling agent decreased significantly, RGR and RFWG in comparison with 3 g l…”

Section: Resultsmentioning

confidence: 99%

Novel medium for enhancing callus growth in hazel (Corylus avellana L.)

Moieni¹,

Salehi²,

Safaie³

2017

J Plant Physiol Pathol

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Paclitaxel is a powerful antimitotic agent with excellent activity against a range of cancers. Hazel has been described as a paclitaxel-producing species among angiosperms. Fast-growing callus is a prerequisite for the success of callus production and then paclitaxel production. Therefore, optimizing the medium culture for enhancing callus growth is a crucial step for paclitaxel production. In this research, Murashige and Skoog (1962) (MS) medium was optimized for improving callus growth of hazel (Corylus avellana L.). The M 10 medium (MS medium with pH 6.0 and supplemented with 1000 mg l −1 spirulina powder, 1000 mg l −1 casein hydrolysate and 3 g l −1 gelrite) significantly improved hazel callus growth. This modified MS medium increased callus fresh weight (55.8%) as compared to the control. M 10 medium increased fatty acids yield of callus (66.7%) as compared to the control. Liquid M 10 medium maintained growth over a longer period of time and also increased slightly, the paclitaxel production as compared to the control. This novel medium is promising for facilitating the mass production of hazel callus as a source of valuable metabolites including paclitaxel, linoleic and oleic acids.Paclitaxel is a powerful antimitotic agent with excellent activity against a range of cancers 1 . The major limitation in the extensive use of this valuable secondary metabolite is its low supply, since Taxus spp. contains very low amounts of paclitaxel 2 . Extraction of paclitaxel from this tree has imposed important ecological effects, resulting in the extinction of Taxus species 3 . Plant cell suspension culture is considered as the most promising approach to the production of paclitaxel 4 . The availability of this drug is still restricted and its cost is very high, mainly due to the recalcitrant behavior of Taxus spp. under in vitro conditions 2 . Therefore, the search for alternative sources of paclitaxel was considered as crucial. In addition to Taxus spp., hazel (Corylus avellana) has also been described as a paclitaxel-producing species through bioprospection among angiosperms 5,6 . The major advantages of producing paclitaxel through hazel cell cultures are that hazel is widely accessible and its in vitro cultivation is easier than that of yew 2,7 . It is stated that in vitro cultures of C. avellana can become a promising and cheaper source for paclitaxel production 8 . Besides the use of the nuts of hazel tree as a source of protein, its leaves are used to relieve the symptoms of hemorrhoidal and varicose veins 9 . The kernel and green leaf/flower portions of the hazel tree have antioxidant activity 10 . It is found that the consumption of nuts is protective against cardiac morbidity and mortality 11. In addition to the use of hazel cell cultures for paclitaxel production, hazel plantlets can be regenerated from callus tissues by differentiation induced by exogenous growth regulators. Plant regeneration from calli is possible by somatic embryogenesis or in vitro organogenesis. Meanwhile, infrequent somaclonal variants...

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Multielement Analysis of Agar and Gelrite Impurities Investigated by Inductively Coupled Plasma Emission Spectrometry as Well as Physical Properties of Tissue Culture Media Prepared With Agar or the Gellan Gum Gelrite

Cited by 43 publications

References 0 publications

The effect of boron on calcium uptake and growth in micropropagated potato plantlets

The effect of boron on calcium uptake and growth in micropropagated potato plantlets

Promotion of root emergence in vitro from rhizome buds of Lapageria rosea cv. Nashcourt after proliferation in the presence of paclobutrazol

Novel medium for enhancing callus growth in hazel (Corylus avellana L.)

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