Multienzymatic immobilization of laccases on polymeric microspheres: A strategy to expand the maximum catalytic efficiency

Vera, Myleidi; Fodor, Csaba; García, Yadiris; Pereira, Eduardo; Loos, Katja; Rivas, Bernabé L.

doi:10.1002/app.49562

Cited by 12 publications

(8 citation statements)

References 46 publications

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“…Co-immobilization of laccases from different sources has also been explored. For example, Vera et al [ 608 ], co-immobilized laccase from Aspergillus sp., M . thermophile , and T .…”

Section: Co-immobilization Of Laccase With Other Enzymes and Substratesmentioning

confidence: 99%

Applications and immobilization strategies of the copper-centred laccase enzyme; a review

Kyomuhimbo¹,

Brink²

2023

Heliyon

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“…Co-immobilization of laccases from different sources has also been explored. For example, Vera et al [ 608 ], co-immobilized laccase from Aspergillus sp., M . thermophile , and T .…”

Section: Co-immobilization Of Laccase With Other Enzymes and Substratesmentioning

confidence: 99%

Applications and immobilization strategies of the copper-centred laccase enzyme; a review

Kyomuhimbo¹,

Brink²

2023

Heliyon

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“…Laccase activity was measured by following the oxidation of the ABTS substrate, as previously reported. 24 Briefly, 50 μL of 10 mM ABTS was mixed with 170 μL of laccase diluted in acetate buffer at pH 5. Absorbance was monitored at 420 nm for 5 min at 25 C with a POLARstar OMEGA multi-plate reader (BMG LABTECH).…”

Section: Laccase Activity Assaymentioning

confidence: 99%

Laccase‐mediated polymerization of tannins from a pine bark extract: Toward an eco‐friendly valorization of forest wastes

Vera,

Silva,

et al. 2024

J of Applied Polymer Sci

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The valorization of tannins from forest by‐products is a major opportunity for the sustainable development of the forestry industry. Herein we report the laccase‐mediated polymerization of tannins obtained from an aqueous pine bark extract. The reaction was conducted under an oxygen atmosphere at 50°C for 60 min and followed by UV–Vis, fluorescence intensity, phenol content, and viscosity measurements. An insoluble polymer was obtained after 45 min, which was attributed to coupling reactions triggered by phenoxyl radicals within the tannins mixture. The polymer showed a marked increase in thermal stability compared with the precursor pine bark extract, with a total mass loss of less than 12% at temperatures up to 500°C, suggesting potential practical applications in flame retardant materials. Additionally, the polymer showed an 85% DPPH activity and a 60% attenuation of singlet oxygen release upon light irradiation, which supports antioxidant and photoprotective properties for the prepared material. To the best of our knowledge, this is the first report to describe the formation of an insoluble polymer by direct enzymatic reaction of an aqueous tannin extract. In this case, soluble tannins are the sole reactants used to produce a promising versatile material for forest waste valorization.

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“…A typical synthesis procedure of microspheres is demonstrated: [ 78 ] The reaction mixture consisted of the GMA monomer, poly(N‐vinylpyrrolidone) (PVP) as the stabilizer, α, α'‐azoisobisbutyronitrile (AIBN) as the initiator, methanol as aqueous medium and N, N‐dimethylformamide. The reaction mixture was deoxygenated with nitrogen and placed in an oil bath to maintain a temperature of 65 °C. The beaker was stirred for 8 h at the rate of 160 rpm. Once the polymerization culminated, the mixture was centrifuged and washed with excess methanol twice.…”

Section: Synthesis Of Acrylic Microspheresmentioning

confidence: 99%

Recent Progress in Intensifying Synthesis of Acrylic Microspheres for Catalysis

Gaddale

Kale

Srinivasan

et al. 2023

Adv Materials Inter

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Over the past decades, there has been an escalating rise in the need for chemicals and catalytic materials to keep up with global demands. Addressing those issues by conventional methods often becomes inefficient, with myriad operational risks. Process intensification methods through procedural and equipment‐based modifications have been considered greener, have higher heat and mass transfer rates, and operate with lower costs. In this review, research using ultrasonic reactors and microreactors, along with developments through an integrated external energy source, for synthesizing acrylic microspheres is covered extensively. Acrylic microspheres have garnered much interest for their biocompatibility, affinity toward functionalization, and wide range of applications. Core–shell, composite, functional‐group modified, and porous acrylic microspheres are used for enzyme immobilization and as catalyst carriers. The use of acrylic support has provided huge improvements in catalytic activity, reusability, recyclability, and overall stability. Finally, various other process intensification methods and alternate support materials are covered to help enhance future developments in the field of catalysis.

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Multienzymatic immobilization of laccases on polymeric microspheres: A strategy to expand the maximum catalytic efficiency

Cited by 12 publications

References 46 publications

Applications and immobilization strategies of the copper-centred laccase enzyme; a review

Applications and immobilization strategies of the copper-centred laccase enzyme; a review

Laccase‐mediated polymerization of tannins from a pine bark extract: Toward an eco‐friendly valorization of forest wastes

Recent Progress in Intensifying Synthesis of Acrylic Microspheres for Catalysis

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