Multifaced Assessment of Antioxidant Power, Phytochemical Metabolomics, In-Vitro Biological Potential and In-Silico Studies of Neurada procumbens L.: An Important Medicinal Plant

Khurshid, Umair; Ahmad, Saeed; Saleem, Hammad; Lodhi, Arslan Hussain; Pervaiz, Irfan; Khan, Mohsin Abbas; Khan, Haroon; Alamri, Abdulwahab; Ansari, Mukhtar; Locatelli, Marcello; Arshad, Muhammad; Wazir, Muhammad Asif; Butt, Juwairiya; Anwar, Sirajudheen

doi:10.3390/molecules27185849

Cited by 2 publications

(2 citation statements)

References 40 publications

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“…Molecular docking is a computer simulation method for investigating the interactions between ligands and receptors, and can be used to predict binding patterns and affinity. In recent years, it has been used to study the bioactivity of enzyme inhibitors from herbal medicine [20][21][22]. In order to verify the activities of seven potential enzyme inhibitors, including methylgallate (1), 1,6-di-O-galloyl-D-glucose (2), polydatin-4 -O-D-glucoside (3), resveratrol-4 -O-D-glucoside (4), polydatin (5), malonyl glucoside resveratrol (6), and resveratrol-5-O-D-glucoside (7), that were screened from PCR extract, a molecular docking analysis of these components with TYR, XOD, and α-GLU was carried out.…”

Section: Molecular Docking Analysismentioning

confidence: 99%

Screening of Tyrosinase, Xanthine Oxidase, and α-Glucosidase Inhibitors from Polygoni Cuspidati Rhizoma et Radix by Ultrafiltration and HPLC Analysis

Chen

Huang

et al. 2023

Molecules

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Polygoni Cuspidati Rhizoma et Radix (PCR), the rhizome and root of Polygonum cuspidatum Sieb. et Zucc., has been used as an herbal medicine for a long time. In this study, the ultrafiltration combined with high performance liquid chromatography (UF-HPLC) method was developed to screen tyrosinase (TYR), α-glucosidase (α-GLU), and xanthine oxidase (XOD) inhibitors from PCR. Firstly, the inhibitory activity of 50% methanol PCR extract on TYR, α-GLU, XOD, and acetylcholinesterase (ACHE) was tested. The extract showed a good inhibition on the enzymes, except for ACHE. Therefore, UF-HPLC experiments were carried out to screen TYR, α-GLU, and XOD inhibitors from PCR extract. Seven potential bioactive components were discovered, including methylgallate (1), 1,6-di-O-galloyl-D-glucose (2), polydatin-4′-O-D-glucoside (3), resveratrol-4′-O-D-glucoside (4), polydatin (5), malonyl glucoside resveratrol (6), and resveratrol-5-O-D-glucoside (7). Most of them were found as enzyme inhibitors from PCR for the first time, except polydatin (5), which had been reported as an α-GLUI in PCR in the literature. Finally, molecular docking analysis was applied to validate the interactions of these seven potential active components with the enzymes. Compounds 1–7 were proven as TYR inhibitors, compounds 2, 4–7 were identified as XOD inhibitors, and compounds 4–6 were confirmed as α-GLU inhibitors. In short, the current study provides a good reference for the screening of enzyme inhibitors through UF-HPLC, and provides scientific data for future studies of PCR.

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Section: Molecular Docking Analysismentioning

confidence: 99%

Screening of Tyrosinase, Xanthine Oxidase, and α-Glucosidase Inhibitors from Polygoni Cuspidati Rhizoma et Radix by Ultrafiltration and HPLC Analysis

Chen

Huang

et al. 2023

Molecules

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“…Several parts of Ziziphus nummularia can be used for the treatment of a broad range of diseases. Recently it has gained scientific approval for possessing beneficial bioactive substances that act as antimicrobial, antioxidant, antitumor, anti-hypotensive, antiinflammatory, anti-hypoglycaemic, liver protective and immune system stimulants (Mustafa et al 2019;Khurshid et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Mitigation Strategies for Vancomycin-resistant Staphylococcus aureus

2023

IJAB

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In just two hundred years the prevalence of infectious disease started soaring as new modes of travelling were invented. Now people could one place to another quickly carrying several kinds of disease with them. With the advent of diseases, life expectancy was reduced to around 35 years and death was rampant. These dire circumstances led to the discovery of antibiotics that became the main weapon of mankind against infectious diseases post-infection. Humanity was saved from the looming threat of constant epidemics and reduced life expectancy. Soon mankind began to thrive and people started increasing the use of antibiotics to battle all kinds of diseases. With overuse came the problem of misuse of antibiotics. People soon started using antibiotics without proper protocols and dosing regimens. This malpractice soon resulted in the emergence of a capability in bacteria to nullify the effects of antibiotics. The antibiotic resistance meant that the easily curable diseases once again became untreatable maladies. One such bacteria that gained antibiotic resistance was Staphylococcus aureus. S. aureus gained resistance against the methicillin group of antibiotics as they were commonly used against it. After that vancomycin became the drug of choice against methicillin-resistant S. aureus. Soon, people started misusing vancomycin too which quickly led to the development of vancomycin-resistant S. aureus (VRSA). VRSA were usually multidrug resistant bacteria that effectively rendered, many of the antibiotics being used against them, ineffective. These conditions forced the researchers to look for alternative medication modes and techniques for countering antibiotic resistance.

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Multifaced Assessment of Antioxidant Power, Phytochemical Metabolomics, In-Vitro Biological Potential and In-Silico Studies of Neurada procumbens L.: An Important Medicinal Plant

Cited by 2 publications

References 40 publications

Screening of Tyrosinase, Xanthine Oxidase, and α-Glucosidase Inhibitors from Polygoni Cuspidati Rhizoma et Radix by Ultrafiltration and HPLC Analysis

Screening of Tyrosinase, Xanthine Oxidase, and α-Glucosidase Inhibitors from Polygoni Cuspidati Rhizoma et Radix by Ultrafiltration and HPLC Analysis

Mitigation Strategies for Vancomycin-resistant Staphylococcus aureus

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