Multifaceted application of nanoparticle-based labeling strategies for stem cell therapy

Engineering of SPECT/Photoacoustic Imaging/Antioxidative Stress Triple-Function Nanoprobe for Advanced Mesenchymal Stem Cell Therapy of Cerebral Ischemia

“…However, these single-modal imaging approaches enable the acquisition of only limited information and barely satisfy the multiple requirements for clinical application of stem cell therapy. 44 In this study, our CPMSN@ 125 I-SD-labeled BMSCs have been demonstrated to exhibit both high-contrast PA imaging ability and 125 I radioactivity, enabling real-time imaging-guided cellular injection via PA imaging and monitoring of the labeled BMSCs under SPECT/CT imaging.…”

“…Several single-modal imaging agents with PA imaging capability (e.g., gold nanoparticles , and Prussian blue nanoparticles) or SPECT imaging capability (e.g., 64 Cu-PTSM, 111 In-oxiquinolon, and 99 mTc-HMPAO) have been utilized as effective stem cell labeling probes in recent years. However, these single-modal imaging approaches enable the acquisition of only limited information and barely satisfy the multiple requirements for clinical application of stem cell therapy . In this study, our CPMSN@ 125 I-SD-labeled BMSCs have been demonstrated to exhibit both high-contrast PA imaging ability and 125 I radioactivity, enabling real-time imaging-guided cellular injection via PA imaging and monitoring of the labeled BMSCs under SPECT/CT imaging.…”

Cobalt protoporphyrin-induced nano-self-assembly for CT imaging, magnetic-guidance, and antioxidative protection of stem cells in pulmonary fibrosis treatment

“…So far, conventional methods of stem cell labeling based on an active or passive intracellular delivery of nanoparticles have been performed, which require a 24 h co-incubation and are time-consuming and less efficient [ 33 , 34 ]. Despite the development of various methods for an efficient labeling of stem cells with nanoagents, such as cationic polymer modification, they still suffer from the potential side effects of hindering stem cell proliferation and differentiation [ 13 ]. In order to improve the MSC labeling efficiency of the nanoagent, meanwhile to ensure good biocompatibility, a bioorthogonal strategy was harnessed in this work ( Fig.…”

“…In parallel, the assessment of stem cell tracking and their bio-distribution is pivotal to ascertain the safety and therapeutic efficacy of MSC therapy [ 12 ]. While a number of imaging modalities based on contrast agents, such as computed tomography (CT), magnetic resonance imaging (MRI) and ultrasound imaging (USI) have been developed for real-time stem cell tracking, they still face several limitations, including poor imaging contrast and sensitivity of the labeling agents, low cell labeling efficiency, and extensive in vitro manipulations of cells, etc [ 13 , 14 ]. Thus, development of a multi-modal strategy that may offer a solution to the above-mentioned issues in stem cell-based PF therapy is highly desired [ 3 ].…”

Emerging New‐Generation Detecting and Sensing of Metal Halide Perovskites

“…Particularly, MHPs with a particle size of less than 200 nm [3,36] are suitable for excretory organs. [2,60,61] Although the Pb 2+ content in several MHPs can cause cytotoxicity, this detrimental effect can be blocked through Reproduced with permission. [20] Copyright 2020, Nature encapsulation using biocompatible nano-shells [3,32,33,36,[62][63][64][65][66] or by special functional ligand modification.…”