Multifaceted Function of Myosin-18, an Unconventional Class of the Myosin Superfamily

Ouyang, Zhaohui; Zhao, Shuangshuang; Yao, Su; Wang, Jing; Cui, Yanqin; Wei, Ke; Jiu, Yaming

doi:10.3389/fcell.2021.632445

Cited by 10 publications

(10 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mutation studies have shown that Dst-b is essential for strained muscle maintenance (Yoshioka et al, 2022). We have also found a node exclusion block in Myo18A, which is consistent with shorter annotated isoforms that are derived from an internal transcription start site (Guzik-Lendrum et al, 2013;Ouyang et al, 2021)…”

Section: Identification Of Coordinately Spliced Exon Blockssupporting

confidence: 82%

Mining alternative splicing patterns in scRNA-seq data using scASfind

Song,

Parada,

Lee

et al. 2023

Preprint

View full text Add to dashboard Cite

Single-cell RNA-seq is widely used for transcriptome profiling, but most analyses have focused on gene-level events, with much less attention devoted to alternative splicing. Here, we present scASfind, a novel computational method to allow for quantitative analysis of cell type-specific splicing events. scASfind utilizes an efficient data structure to store the percent spliced-in value for each splicing event. This makes it possible to exhaustively search for patterns among all differential splicing events, allowing us to identify marker events, mutually exclusive events, and large blocks of exons that are specific to one or more cell types. These methods allow researchers to compare cells based on isoforms rather than genes, thereby enabling more nuanced characterization of cell types and states. We demonstrate the advantages of scASfind on two mouse and one human datasets, identifying differences across the several key genes that cannot be detected using gene expression alone.

show abstract

Section: Identification Of Coordinately Spliced Exon Blockssupporting

confidence: 82%

Mining alternative splicing patterns in scRNA-seq data using scASfind

Song,

Parada,

Lee

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…These observations further support an implication of Lurap1 in connecting actin cytoskeleton to cell adhesion. Given the importance of MYO18A and p190RhoGEF in the regulation of the actin-based contractile machinery in different cell types [ 37 , 38 , 39 ], it would be of interest to determine whether these Lurap1 interaction partners are also involved in epiboly movement.…”

Section: Discussionmentioning

confidence: 99%

The Adaptor Protein Lurap1 Is Required for Cell Cohesion during Epiboly Movement in Zebrafish

Cheng

Zhang

et al. 2021

Biology

View full text Add to dashboard Cite

Cell adhesion and polarized cellular behaviors play critical roles in a wide variety of morphogenetic events. In the zebrafish embryo, epiboly represents an important process of epithelial morphogenesis that involves differential cell adhesion and dynamic cell shape changes for coordinated movements of different cell populations, but the underlying mechanism remains poorly understood. The adaptor protein Lurap1 functions to link myotonic dystrophy kinase-related Rac/Cdc42-binding kinase with MYO18A for actomyosin retrograde flow in cell migration. We previously reported that it interacts with Dishevelled in convergence and extension movements during gastrulation. Here, we show that it regulates blastoderm cell adhesion and radial cell intercalation during epiboly. In zebrafish mutant embryos with loss of both maternal and zygotic Lurap1 function, deep cell multilayer of the blastoderm exhibit delayed epiboly with respect to the superficial layer. Time-lapse imaging reveals that these deep cells undergo unstable intercalation, which impedes their expansion over the yolk cell. Cell sorting and adhesion assays indicate reduced cellular cohesion of the blastoderm. These defects are correlated with disrupted cytoskeletal organization in the cortex of blastoderm cells. Thus, the present results extend our previous works by demonstrating that Lurap1 is required for cell adhesion and cell behavior changes to coordinate cell movements during epithelial morphogenesis. They provide insights for a further understanding of the regulation of cytoskeletal organization during gastrulation cell movements.

show abstract

“…The myosin‐XVIII family is represented by Myo18a and Myo18b, with the latter being found exclusively in vertebrates (Salamon et al, 2003). Initially identified by the presence of a PDZ domain in its N‐terminal region, the myosin‐XVIII class has more recently been defined by typical long N‐ and C‐terminal extensions (Ouyang et al, 2021).…”

Section: Myosin‐xviiimentioning

confidence: 99%

“…While most myosins are known for their ability to hydrolyze ATP into ADP and use the resulting energy to generate movement and tension along actin filaments, MYO18A and MYO18B have mutations in the regions coding for the motor domain responsible for ATP hydrolysis. In addition, biochemical assays have shown that Myo18a lacks ATPase activity (Guzik‐Lendrum, Nagy, Takagi, Houdusse, & Sellers, 2011; Ouyang et al, 2021; Taft et al, 2013). The matter is not entirely settled, though, as some reports have indicated that mutations in the Myo18a motor domain can turn the resulting protein into a dominant‐negative (Tan, Yong, Dong, Lim, & Leung, 2008), suggesting the motor domain may be active.…”

Section: Myosin‐xviiimentioning

confidence: 99%

Myosin motors on the pathway of viral infections

2022

View full text Add to dashboard Cite

Molecular motors are microscopic machines that use energy from adenosine triphosphate (ATP) hydrolysis to generate movement. While kinesins and dynein are molecular motors associated with microtubule tracks, myosins bind to and move on actin filaments. Mammalian cells express several myosin motors. They power cellular processes such as endo‐ and exocytosis, intracellular trafficking, transcription, migration, and cytokinesis. As viruses navigate through cells, they may take advantage or be hindered by host components and machinery, including the cytoskeleton. This review delves into myosins' cell roles and compares them to their reported functions in viral infections. In most cases, the previously described myosin functions align with their reported role in viral infections, although not in all cases. This opens the possibility that knowledge obtained from studying myosins in viral infections might shed light on new physiological roles for myosins in cells. However, given the high number of myosins expressed and the variety of viruses investigated in the different studies, it is challenging to infer whether the interactions found are specific to a single virus or can be applied to other viruses with the same characteristics. We conclude that the participation of myosins in viral cycles is still a largely unexplored area, especially concerning unconventional myosins.

show abstract

Multifaceted Function of Myosin-18, an Unconventional Class of the Myosin Superfamily

Cited by 10 publications

References 53 publications

Mining alternative splicing patterns in scRNA-seq data using scASfind

Mining alternative splicing patterns in scRNA-seq data using scASfind

The Adaptor Protein Lurap1 Is Required for Cell Cohesion during Epiboly Movement in Zebrafish

Myosin motors on the pathway of viral infections

Contact Info

Product

Resources

About