Multifunctional Biosensor Based on Localized Surface Plasmon Resonance for Monitoring Small Molecule–Protein Interaction

Guerreiro, J. Rafaela L.; Frederiksen, Maj; Bochenkov, Vladimir E.; Freitas, Víctor de; Sales, M. Goreti F.; Sutherland, Duncan S.

doi:10.1021/nn501962y

Cited by 62 publications

(52 citation statements)

References 67 publications

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“…[1][2][3][4][5][6][7][8][9][10][11][12][13] These nanoparticles display unique localized surface plasmon resonance (LSPR) properties 1,4,6,13 originating from the coherent oscillation of conduction electrons on their surface when excited by incident photons. In addition to size and shape of a nanoparticle, the local dielectric environment modulates its LSPR frequency.…”

Section: Introductionmentioning

confidence: 99%

Achieving biosensing at attomolar concentrations of cardiac troponin T in human biofluids by developing a label-free nanoplasmonic analytical assay

Liyanage

Sangha

Sardar

2017

Analyst

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Acute myocardial infarction (heart attack) is the fifth leading cause of death in the United States (Dariush et al. Circulation 2015, 131, e29-e322). This highlights the needs for early, rapid, and sensitive detection of its occurrence and severity through assaying cardiac biomarkers in human fluids. Herein we report chip-based fabrication of the first label-free, nanoplasmonic biosensor to assay cardiac Troponin T (cTnT) in human biofluids (plasma, serum, and urine) with high specificity. The sensing mechanism is based on the adsorption model that measures the localized surface plasmon resonance (LSPR) wavelength shift of anti-cTnT functionalized gold triangular nanoprisms (Au TNPs) induced by change of their local dielectric environment upon binding of cTnT. We demonstrate that controlled manipulation of sensing volume and decay length of Au TNPs together with the appropriate surface functionalization and immobilization of anti-cTnT onto TNPs allows us to achieve a limit of detection (LOD) of our cTnT assay at attomolar concentration (~15 aM) in human plasma. This LOD is at least 50 fold more sensitive than that of other label-free techniques. Furthermore, we demonstrate excellent sensitivity of our sensors in human serum and urine. Importantly, the strategy of our chip-based fabrication is extremely reproducible. We believe our powerful analytical tool for detection of cTnT directly in human biofluids using this highly reproducible, label-free LSPR sensor will have great potential for early diagnosis of heart attack and thus increase patients' survival rate.

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Section: Introductionmentioning

confidence: 99%

Achieving biosensing at attomolar concentrations of cardiac troponin T in human biofluids by developing a label-free nanoplasmonic analytical assay

Liyanage

Sangha

Sardar

2017

Analyst

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“…38,39 It is well established that the refractive index of the surrounding environment affects the wavelength of the LSPR. 37 To ascertain the optimal conditions for the aptamer-based assays, the sensitives of LSPR measurements in liquid (buffer) and air were compared by measuring the LSPR wavelength shift (Δ λ ) at several points in the assembly and capture process in both PBS and air.…”

Section: Resultsmentioning

confidence: 99%

Whole-Cell Pseudomonas aeruginosa Localized Surface Plasmon Resonance Aptasensor

Bohn

2018

Anal. Chem.

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The detection of whole-cell Pseudomonas aeruginosa presents an intriguing challenge with direct applications in health care and the prevention of nosocomial infection. To address this problem, a localized surface plasmon resonance (LSPR) based sensing platform was developed to detect whole-cell Pseudomonas aeruginosa strain PAO1 using a surface-confined aptamer as an affinity reagent. Nanosphere lithography (NSL) was used to fabricate a sensor surface containing a hexagonal array of Au nanotriangles. The sensor surface was subsequently modified with biotinylated polyethylene glycol (Bt-PEG) thiol/PEG thiol (1:3), neutravidin, and biotinylated aptamer in a sandwich format. The 1:3 (v/v) ratio of Bt-PEG thiol/PEG thiol was specifically chosen to maximize PAO1 binding while minimizing nonspecific adsorption and steric hindrance. In contrast to prior whole-cell LSPR work, the LSPR wavelength shift was shown to be linearly related to bacterial concentration over the range of 10–103 cfu mL−1. This LSPR sensing platform is rapid (~3 h for detection), sensitive (down to the level of a single bacterium), selective for detection of Pseudomonas strain PAO1 over other strains, and exhibits a clinically relevant dynamic range and excellent shelf life (≥2 months) when stored at ambient conditions. This versatile LSPR sensing platform should be extendable to a wide range of supermolecular analytes, including both bacteria and viruses, by switching affinity reagents, and it has potential to be used in point-of-care and field-based applications.

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“…Both theoretical and experimental results in the literature have shown that SPR peak position depends on particle diameter, aspect ratio, shape, surface coating, surfactants, surface morphology, volume, coating of other nanoparticles, and aggregates of gold substrates. [45][46][47][48][49][50][51][52][53][54] Sigmoidal responses were observed as a function of the coverage of proteins (in log scale) on gold particles deposited on flat substrates. 55 This is similar to the Langmuir-Freundlich isotherm curve, 47 which is not an intrinsic response of silver layer on gold substrate.…”

Section: Resultsmentioning

confidence: 99%

Sub-monolayer silver loss from large gold nanospheres detected by surface plasmon resonance in the sigmoidal region

Lien

Peck

et al. 2016

Journal of Colloid and Interface Science

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Nanosilver becomes labile upon entering the human body or the environment. This lability creates silver species with antimicrobial properties that make nanosilver attractive as active components in many consumer products, wound dressings, and agricultural applications. Because lability depends strongly on morphology, it is imperative to use a material with constant lability throughout kinetic studies so that accurate lability data can be acquired with efficient detection. Here 2.5nm thick silver was coated onto 90-nm diameter gold nanosphere cores and this surface silver layer was gradually removed by either chemical or X-ray radiation etching. The most sensitive region of a sigmoidal surface plasmon resonance (SPR) response as a function of silver thickness was found for the first time between 0.9- and 1.6-nm thick silver, revealing a new nanosilver standard for lability studies. The SPR peak position detection sensitivity is 8nm (SPR peak shift)/nm (silver thickness change) within this steepest region of the plasmon response curve whereas outside, sensitivity drops to 1nm/nm. Since the centroid of SPR profiles can be discerned with 0.25nm precision, the 8-nm/nm sensitivity means it is possible to detect a 0.3-angstrom or sub-monolayer change in silver thickness. The SPR response simulated by discrete dipole approximation (DDA) was an identical sigmoidal function between 0 and 2nm of silver coating. These findings were supported by several other analytical measurements, which confirmed no silver recoating during these etching processes.

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Multifunctional Biosensor Based on Localized Surface Plasmon Resonance for Monitoring Small Molecule–Protein Interaction

Cited by 62 publications

References 67 publications

Achieving biosensing at attomolar concentrations of cardiac troponin T in human biofluids by developing a label-free nanoplasmonic analytical assay

Achieving biosensing at attomolar concentrations of cardiac troponin T in human biofluids by developing a label-free nanoplasmonic analytical assay

Whole-Cell Pseudomonas aeruginosa Localized Surface Plasmon Resonance Aptasensor

Sub-monolayer silver loss from large gold nanospheres detected by surface plasmon resonance in the sigmoidal region

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