Multifunctional fluorescent probes for high-throughput characterization of hexosaminidase enzyme activity

Chemical biology tools to probe bacterial glycans

“…Using a similar activity-based profiling strategy, Killinger et al employed a glucose-iodoacetamide probe bearing an azide at the 6-position [ 64 ] to crosslink and detect Bifidobacterium enzymes involved in mucin degradation [ 65 ]. High-throughput fluorogenic probes developed by Wang et al to detect glucosidase activity [ 66 ] and poly- N -acetylglucosamine (PNAG)-degradation [ 67 ] further expanded the available toolkit ( Figure 3d ). Access to these probes, coupled to rigorous downstream biochemical characterization of reconstituted enzymes [ 68 , 69 ], opens the door to identifying bacterial glycan-active enzymes that modulate a variety of biological processes, from host immune detection to biofilm degradation.…”

Discrimination of Cell Death Types with an Activatable Fluorescent Probe through Visualizing the Lysosome Morphology

“…Some works tried to endow the probe with an intrinsic lysosome anchoring ability to overcome above deficiencies, but this is often interfered with by background fluorescence . On the other hand, the common recognition substrate for Hexs is N-acetyl-β- d -glucosaminide (GlcNAc), , yet, current fluorescent probes based on this substrate have some disadvantages, such as complex synthetic routes, poor water solubility, low detection sensitivity, short emission wavelength, or a small Stokes shift, which limit their application in vivo . − Therefore, it is a great challenge for designing a fluorescent probe to spontaneously localize the lysosome and specifically detect Hexs.…”