Multifunctional fractionation of polyclonal antibodies by immunoaffinity high-performance monolithic disk chromatography

Ostryanina, Natalia D; Vlasov, G. P.; Tennikova, Tatiana

doi:10.1016/s0021-9673(02)00007-9

Cited by 57 publications

(41 citation statements)

References 26 publications

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“…The cartridge allows insertion of several 3 mm thick disks. This stacking ability enables so-called conjoin separation processes in which disks with different functionalities are used simultaneously [89]. A similar stacking approach can also be used to enhance the total adsorption capacity and thus the throughput of the separation system.…”

Section: Different Formats Of Monolithsmentioning

confidence: 99%

Preparation of methacrylate monoliths

Влах

Tennikova²

2007

J of Separation Science

151

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Rigid macroporous polymers developed in the early 1990s are widely used as efficient stationary phases for all types of chromatographic separations. The main advantages of so-called monolithic supports are their high hydraulic permeability and the dominance of the convection over the diffusion mechanism of mass-exchange under dynamic conditions that allow the separation to be carried out at extremely high flow rates and, consequently, during very short operation times. Among other types of macroporous polymers, the methacrylate-based monolithic materials represent the most popular and successfully explored class of sorbents. This review is an attempt to collect together the contributions of different groups working in the area of monolith preparation. Examples of different methcrylate monomers and crosslinkers, as well as porogenic solvents, including polymer ones, used in monolith preparation are discussed.

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Section: Different Formats Of Monolithsmentioning

confidence: 99%

Preparation of methacrylate monoliths

Влах

Tennikova²

2007

J of Separation Science

151

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“…Incubate the disk in a vial with immobilization solution at room temperature for a 20-24 h without any stirring (static immobilization method) [16,[18][19][20]26]. …”

Section: Methodsmentioning

confidence: 99%

“…Mixtures of polyclonal antibodies were produced by immunization of rabbit with complex antigen, namely, the conjugate BSA-S-BK [16,26,29]. The affinity monolithic disk with immobilized hormone bradykinin was applied for fast isolation of antibodies from a blood serum preliminary purified by triple precipitation with 32-35 % aqueous ammonium sulfate [30], as well as from a crude blood serum [16,26]. In the first case, the chromatographic procedure includes the following steps:…”

Section: Test Of Nonspecific Interactionsmentioning

confidence: 99%

Affinity Chromatography of Proteins on Monolithic Columns

Влах

Platonova

Tennikova

2014

Methods in Molecular Biology

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At present, monolithic stationary phases, because of their morphology, are widely used for development and realization of fast dynamic and static processes based on mass transition between liquid and solid phases. These are liquid chromatography, solid phase synthesis, microarrays, flow-through enzyme reactors, etc. High-performance liquid chromatography on monoliths, including bioaffinity mode, represents a unique technique appropriate for fast and efficient separation of biological (macro)molecules of different sizes and shapes (proteins, nucleic acids, peptides), as well as such supramolecular systems as viruses.In this work, the examples of application of commercially available macroporous monoliths for modern affinity processing are presented. In particular, the original methods developed for efficient isolation and fractionation of monospecific antibodies from rabbit blood sera, the possibility of simultaneous affinity separation of protein G and serum albumin from human serum, the isolation of recombinant products, such as protein G and tissue plasminogen activator from E. coli cell lysate and Chinese Hamster Ovary cell culture supernatant, respectively, are described in detail. The suggested and realized multifunctional fractionation of polyclonal pools of antibodies by combination of several short monolithic columns (disks) with different affinity functionalities stacked in the same cartridge represents an original and practically valuable method that can be used in biotechnology.

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“…This approach can be used directly with GMA/ EDMA monoliths, since epoxy groups are present as the functional groups of the GMA monomers. This coupling technique has been used with monolithic disks to immobilize protein G [40,41], protein A [40,76,77], protein L [40], BSA [42], and bradykinin [42]. It has also been used with GMA/EDMA columns to immobilize L-histidine [76], HSA [4,36], and antibodies [36].…”

Section: Covalent Immobilization Methodsmentioning

confidence: 99%

“…The same study examined the use of multidimensional chromatography on monoliths for the isolation of a monoclonal antibody from ascites fluid by using tandem disks containing an anion-exchange material or immobilized protein A. A separate report used immobilized BSA and related derivatives along with bradykinin as ligands on GMA/EDMA disks for the rapid separation of antibodies from rabbits that had been immunized with complex peptide-protein conjugates [42].…”

Section: Immunoaffinity Chromatography and Related Methodsmentioning

confidence: 99%

Affinity monolith chromatography

Mallik

Hage²

2006

J of Separation Science

190

282

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The combined use of monolithic supports with selective affinity ligands as stationary phases has recently given rise to a new method known as affinity monolith chromatography (AMC). This review will discuss the basic principles behind AMC and examine the types of supports and ligands that have been employed in this method. Approaches for placing affinity ligands in monoliths will be considered, including methods based on covalent immobilization, biospecific adsorption, entrapment, and the formation of coordination complexes. Several reported applications will then be presented, such as the use of AMC for bioaffinity chromatography, immunoaffinity chromatography, immobilized metal-ion affinity chromatography, dye-ligand affinity chromatography, and biomimetic chromatography. Other applications that will be discussed are chiral separations and studies of biological interactions based on AMC.

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Multifunctional fractionation of polyclonal antibodies by immunoaffinity high-performance monolithic disk chromatography

Cited by 57 publications

References 26 publications

Preparation of methacrylate monoliths

Preparation of methacrylate monoliths

Affinity Chromatography of Proteins on Monolithic Columns

Affinity monolith chromatography

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