Multifunctional gadolinium-based dendritic macromolecules as liver targeting imaging probes

Luo, Kui; Liu, Gang; He, Bin; Wu, Yao; Gong, Qingyong; Song, Bin; Ai, Hua; Gu, Zhongwei

doi:10.1016/j.biomaterials.2010.12.049

Cited by 63 publications

(35 citation statements)

References 48 publications

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“…Recent work from Luo et al (2011) shows a proof of concept and supports our hypothesis that a composite from PEDOT and CNT may yield stable microelectrodes. In contrast, this paper aims at (i) statistically consolidating the electrochemical data, (ii) comparing PEDOT–CNT to state-of-the-art microelectrodes as well as to pure PEDOT, and (iii) in particular emphasizing the suitability in applications by not only showing biocompatibility of the material itself but also functional recording from cells in vitro .…”

Section: Introductionsupporting

confidence: 85%

“…The CNTs undergo “polymer-wrapping” by the polyanion PSS thus making the suspension of CNT in water possible without any covalent functionalization (O’Connell et al, 2001). In this way, no washing and drying steps are required as compared to other methods (Luo et al, 2011). UV–Vis spectroscopy was employed to obtain a measure of CNT concentration in the suspension.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

PEDOT–CNT Composite Microelectrodes for Recording and Electrostimulation Applications: Fabrication, Morphology, and Electrical Properties

Gerwig¹,

Fuchsberger²,

Schroeppel³

et al. 2012

Front. Neuroeng.

159

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Composites of carbon nanotubes and poly(3,4-ethylenedioxythiophene, PEDOT) and layers of PEDOT are deposited onto microelectrodes by electropolymerization of ethylenedioxythiophene in the presence of a suspension of carbon nanotubes and polystyrene sulfonate. Analysis by FIB and SEM demonstrates that CNT–PEDOT composites exhibit a porous morphology whereas PEDOT layers are more compact. Accordingly, capacitance and charge injection capacity of the composite material exceed those of pure PEDOT layers. In vitro cell culture experiments reveal excellent biocompatibility and adhesion of both PEDOT and PEDOT–CNT electrodes. Signals recorded from heart muscle cells demonstrate the high S/N ratio achievable with these electrodes. Long-term pulsing experiments confirm stability of charge injection capacity. In conclusion, a robust fabrication procedure for composite PEDOT–CNT electrodes is demonstrated and results show that these electrodes are well suited for stimulation and recording in cardiac and neurophysiological research.

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Section: Introductionsupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

PEDOT–CNT Composite Microelectrodes for Recording and Electrostimulation Applications: Fabrication, Morphology, and Electrical Properties

Gerwig¹,

Fuchsberger²,

Schroeppel³

et al. 2012

Front. Neuroeng.

159

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show abstract

“…Although radionuclide-based imaging has poor spacial and anatomical resolution, the nuclear imaging does have high sensitivity with the sub nM to pM concentration of tracer, (26) and these methods are suitable for tumor screening (particular whole body scans) and for patients who cannot undergo or refuse to accept MRI. Given the small number of reports on Gd-based imaging probes homing (27)(28)(29)(30) and the absence of in vivo imaging of hTERT expression with MR antisense technology in recent years, in the present study, we synthesized DOTA-ASON and labeled it with Gd 3+ for targeted imaging of hTERT expression in malignant tumors. The stability, cell uptake rate, biodistribution and tumor accumulation of Gd-DOTA-ASON were similar to those of 99m Tc-MAG3-ASON, due to some shared affinity to plasma albumin and physiochemical in serum between MAG3 and DOTA.…”

Section: Discussionmentioning

confidence: 99%

Magnetic resonance tumor targeting imaging using gadolinium labeled human telomerase reverse transcriptase antisense probes

Ren

Yang²,

Zhu

et al. 2012

Cancer Science

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To develop a molecular probe for MRI detection of human tumor telomerase reverse transcriptase (hTERT) mRNA expression. Uniformly phosphorothioate‐modified hTERT antisense oligonucleotide (ASON) homing hTERT mRNA was labeled with gadolinium (Gd) through the bifunctional chelator 1,4,7, 10‐tetraazacyclododecane‐N, N', N'', N'''‐tetraacetic acid (DOTA) stirred within 45 minutes at 60°C. The Gd labeled probes were characterized in vitro. The cellular uptake rate and biodistribution of 99mTc‐DOTA‐ASON was measured instead of that of Gd‐DOTA‐ASON. A549 lung adenocarcinoma model was established in BALB/c nude mice and Gd‐DOTA‐ASON was injected intraperitoneally and MR images were acquired using 7.0T Micro‐MRI (Bruker Biospec, Ettlingen, Germany) at different time points. Immunohistochemical analysis of telomerase activity of each xenograft was operated two days after in vivo imaging. The binding efficiency of Gd‐DOTA‐ASON reached as high as 71.7 ± 4.5% (n = 6). Gd‐DOTA‐ASON displayed perfect stability in fresh human serum at 37°C for 24 h. Compared with normal lung cells, A549 cells showed an obviously higher uptake of 99mTc‐DOTA‐ASON than that of lung cells (10.5 ± 2.7% vs. 4.8 ± 2.6%, P < 0.05). The signal intensity of A549 xenografts can be enhanced by Gd‐DOTA‐ASON and the signal to noise ratio (SNR) of tumor to muscle reached 2.37 and maintained a relatively high level within 6 h after injection. The activity of hTERT in A549 tumors can be suppressed by Gd‐DOTA‐ASON in pathological slices. The results of this study show that Gd‐DOTA‐ASON can be a promising intracellular MR contrast probe for targeting telomerase‐positive carcinomas. (Cancer Sci, doi: 10.1111/j.1349‐7006.2012.02316.x, 2012)

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“…2 However, these agents are not very good at differentiating diseased (or malfunctioning) tissues from normal ones, because they circulate and distribute nonspecifically throughout the body after injection and would be eliminated quickly by glomerular filtration. [3][4][5] Thus, there is a great need for development of more sensitive and target-specific functional contrast agents to improve the accuracy of diagnosis using MRI.…”

Section: Introductionmentioning

confidence: 99%

Recombinant high-density lipoprotein nanoparticles containing gadolinium-labeled cholesterol for morphologic and functional magnetic resonance imaging of the liver

Rui

Guo²,

Ding³

et al. 2012

IJN

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Background: Natural high-density lipoproteins (HDL) possess important physiological functions to the transport of cholesterol from the peripheral tissues to the liver for metabolic degradation and excretion in the bile. Methods and results:In this work, we took advantage of this pathway and prepared two different gadolinium (Gd)-DTPA-labeled cholesterol-containing recombinant HDL nanoparticles (Gd-chol-HDL) and Gd-(chol) 2 -HDL as liver-specific magnetic resonance imaging (MRI) contrast agents. The reconstituted HDL nanoparticles had structural similarity to native HDL, and could be taken up by HepG2 cells via interaction with HDL receptors in vitro. In vivo MRI studies in rats after intravenous injections of 10 µmol gadolinium per kg of recombinant HDL nanoparticles indicated that both nanoparticles could provide signal enhancement in the liver and related organs. However, different T 1 -weighted image details suggested that they participated in different cholesterol metabolism and excretion pathways in the liver. Conclusion: Such information could be highly useful to differentiate functional changes as well as anatomic differences in the liver. These cholesterol-derived contrast agents and their recombinant HDL preparations may warrant further development as a new class of contrast agents for MRI of the liver and related organs.

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Multifunctional gadolinium-based dendritic macromolecules as liver targeting imaging probes

Cited by 63 publications

References 48 publications

PEDOT–CNT Composite Microelectrodes for Recording and Electrostimulation Applications: Fabrication, Morphology, and Electrical Properties

PEDOT–CNT Composite Microelectrodes for Recording and Electrostimulation Applications: Fabrication, Morphology, and Electrical Properties

Magnetic resonance tumor targeting imaging using gadolinium labeled human telomerase reverse transcriptase antisense probes

Recombinant high-density lipoprotein nanoparticles containing gadolinium-labeled cholesterol for morphologic and functional magnetic resonance imaging of the liver

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