Multifunctional iron oxide-carbon hybrid nanoparticles for targeted fluorescent/MR dual-modal imaging and detection of breast cancer cells

Han, Cuiping; Zhang, Aiwei; Kong, Ying; Yu, Nana; Xie, Ting; Dou, Binru; Li, Ké; Wang, Yong; Li, Jingjing; Xu, Kai

doi:10.1016/j.aca.2019.03.054

Cited by 45 publications

(27 citation statements)

References 54 publications

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“…Up to the present time, different sensors and platforms were developed to detect CD44-expressing cells. This includes quartz crystal microbalance-based sensor 5 and magnetic-fluorescent iron oxide–carbon hybrid nanoparticles 6 and colorimetric nanobiosensor 7 for the detection of CD44 + breast cancer cells, functionalized interdigitated electrodes for prostate cancer cells expressing CD44 8 , functionalized stainless steel wire to capture breast CSC 9 among others.…”

Section: Introductionmentioning

confidence: 99%

Label-free fiber-optic spherical tip biosensor to enable picomolar-level detection of CD44 protein

Bekmurzayeva

Ashikbayeva

Myrkhiyeva

et al. 2021

Sci Rep

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Increased level of CD44 protein in serum is observed in several cancers and is associated with tumor burden and metastasis. Current clinically used detection methods of this protein are time-consuming and use labeled reagents for analysis. Therefore exploring new label-free and fast methods for its quantification including its detection in situ is of importance. This study reports the first optical fiber biosensor for CD44 protein detection, based on a spherical fiber optic tip device. The sensor is easily fabricated from an inexpensive material (single-mode fiber widely used in telecommunication) in a fast and robust manner through a CO2 laser splicer. The fabricated sensor responded to refractive index change with a sensitivity of 95.76 dB/RIU. The spherical tip was further functionalized with anti-CD44 antibodies to develop a biosensor and each step of functionalization was verified by an atomic force microscope. The biosensor detected a target of interest with an achieved limit of detection of 17 pM with only minor signal change to two control proteins. Most importantly, concentrations tested in this work are very broad and are within the clinically relevant concentration range. Moreover, the configuration of the proposed biosensor allows its potential incorporation into an in situ system for quantitative detection of this biomarker in a clinical setting.

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Section: Introductionmentioning

confidence: 99%

Label-free fiber-optic spherical tip biosensor to enable picomolar-level detection of CD44 protein

Bekmurzayeva

Ashikbayeva

Myrkhiyeva

et al. 2021

Sci Rep

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“…Particularly in the biomedical field, when designed to be injected intravenously, the particles will face the recognition and capture by the macrophages of the immune system [5,6], hence the need to make them biomimetic by coating with, for instance, poly(ethylene glycol) (PEG) [7], dextran [8] or chitosan [9]. Of comparable importance is their transformation into specific-targeting vehicles designed to approach diseased cells and not healthy ones [10]; as examples, we can cite the conjugation with CD44 monoclonal antibodies for specific binding to 4T1 breast cancer cells [11], and the overexpression of folate or biotin receptors in some tumor cells, suggesting the use of folic acid or biotin on the particles for preferential (rarely, exclusive) linkage to cancer cells. The number of possible ligands is certainly large, as has been summarized by Loomis et al [12].…”

Section: Introductionmentioning

confidence: 99%

Hyperthermia-Triggered Doxorubicin Release from Polymer-Coated Magnetic Nanorods

et al. 2019

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In this paper, it is proposed that polymer-coated magnetic nanorods (MNRs) can be used with the advantage of a double objective: first, to serve as magnetic hyperthermia agents, and second, to be used as magnetic vehicles for the antitumor drug doxorubicin (DOX). Two different synthetic methodologies (hydrothermal and co-precipitation) were used to obtain MNRs of maghemite and magnetite. They were coated with poly(ethyleneimine) and poly(sodium 4-styrenesulfonate), and loaded with DOX, using the Layer-by-Layer technique. Evidence of the polymer coating and the drug loading was justified by ATR-FTIR and electrophoretic mobility measurements, and the composition of the coated nanorods was obtained by a thermogravimetric analysis. The nanorods were tested as magnetic hyperthermia agents, and it was found that they provided sufficiently large heating rates to be used as adjuvant therapy against solid tumors. DOX loading and release were determined by UV-visible spectroscopy, and it was found that up to 50% of the loaded drug was released in about 5 h, although the rate of release could be regulated by simultaneous application of hyperthermia, which acts as a sort of external release-trigger. Shape control offers another physical property of the particles as candidates to interact with tumor cells, and particles that are not too elongated can easily find their way through the cell membrane.

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“…From these results, specifically by the MRI data from our transgenic HER-2 mouse model, it shows persistence of signal enhancement out to 24 h after initial injection, similar to earlier time points at 4 and 8 h after injection for both CT and MRI. Though imaging agents have proved to be an invaluable tool, circulation time and biodistribution of these agents are concerns [38,39]. This is because increasing circulation times and extending the signal enhancement window, usually results in imaging agents being retained in untargeted organs.…”

Section: Discussionmentioning

confidence: 99%

Functionalized nanoparticles with targeted antibody to enhance imaging of breast cancer in vivo

Chen

Bhattacharjee

et al. 2020

J Nanobiotechnol

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Background Targeted contrast nanoparticles for breast tumor imaging facilitates early detection and improves treatment efficacy of breast cancer. This manuscript reports the development of an epidermal growth factor receptor-2 (HER-2) specific, bi-modal, dendrimer conjugate to enhance computed tomography (CT) and magnetic resonance imaging (MRI) of HER-2-positive breast cancer. This material employs generation 5 poly(amidoamine) dendrimers, encapsulated gold nanoparticles, chelated gadolinium, and anti-human HER-2 antibody to produce the nanoparticle contrast agent. Results Testing in two mouse tumor models confirms this contrast agent’s ability to image HER-2 positive tumors. Intravenous injection of this nanoparticle in mice bearing HER-2 positive mammary tumors significantly enhances MRI signal intensity by ~ 20% and improves CT resolution and contrast by two-fold. Results by flow cytometry and confocal microscopy validate the specific targeting of the conjugate and its internalization in human HER-2 positive cells. Conclusion These results demonstrate that this nanoparticle conjugate can efficiently target and image HER-2 positive tumors in vivo and provide a basis for the development of this diagnostic tool for early detection, metastatic assessment and therapeutic monitoring of HER-2 positive cancers.

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Multifunctional iron oxide-carbon hybrid nanoparticles for targeted fluorescent/MR dual-modal imaging and detection of breast cancer cells

Cited by 45 publications

References 54 publications

Label-free fiber-optic spherical tip biosensor to enable picomolar-level detection of CD44 protein

Label-free fiber-optic spherical tip biosensor to enable picomolar-level detection of CD44 protein

Hyperthermia-Triggered Doxorubicin Release from Polymer-Coated Magnetic Nanorods

Functionalized nanoparticles with targeted antibody to enhance imaging of breast cancer in vivo

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