Multifunctional magnetic-responsive hydrogels to engineer tendon-to-bone interface

Silva, Elsa D.; Babo, Pedro S.; Costa‐Almeida, Raquel; Domingues, Rui M. A.; Mendes, Bárbara B.; Paz, Elvira; Freitas, P. P.; Rodrigues, Márcia T.; Granja, Pedro L.; Gomes, Manuela E.

doi:10.1016/j.nano.2017.06.002

Cited by 79 publications

(62 citation statements)

References 100 publications

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“…High molecular weight (1.5-1.8 MDa) hyaluronic acid (HA; S. equii, Sigma-Aldrich, Missouri, USA) was methacrylated following a previously stablished protocol [18,19,28]. In brief, 1% (w/v) HA solution (in distilled water) was reacted with methacrylic anhydride (10 times molar excess) at pH 8.0-8.5, adjusted with NaOH 5 M (Sigma-Aldrich, Missouri, USA), at 4°C.…”

Section: Production Of Methacrylated Hyaluronic Acidmentioning

confidence: 99%

Hyaluronic acid hydrogels incorporating platelet lysate enhance human pulp cell proliferation and differentiation

Almeida

Babo

Silva

et al. 2018

J Mater Sci: Mater Med

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The restoration of dentine-pulp complex remains a challenge for dentists; nonetheless, it has been poorly addressed. An ideal system should modulate the host response, as well as enable the recruitment, proliferation and differentiation of relevant progenitor cells. Herein was proposed a photocrosslinkable hydrogel system based on hyaluronic acid (HA) and platelet lysate (PL). PL is a cocktail of growth factors (GFs) and cytokines involved in wound healing orchestration, obtained by the cryogenic processing of platelet concentrates, and was expected to provide the HA hydrogels specific biochemical cues to enhance pulp cells' recruitment, proliferation and differentiation. Stable HA hydrogels incorporating PL (HAPL) were prepared after photocrosslinking of methacrylated HA (Met-HA) previously dissolved in PL, triggered by the Ultra Violet activated photoinitiator Irgacure 2959. Both the HAPL and plain HA hydrogels were shown to be able to recruit cells from a cell monolayer of human dental pulp stem cells (hDPSCs) isolated from permanent teeth. The hDPCs were also seeded directly over the hydrogels (5 × 10 cells/hydrogel) and cultured in osteogenic conditions. Cell metabolism and DNA quantification were higher, in all time-points, for PL supplemented hydrogels (p < 0,05). Alkaline phosphatase (ALPL) activity and calcium quantification peaks were observed for the HAPL group at 21 days (p < 0,05). The gene expression for ALPL and COLIA1 was up-regulated at 21 days to HAPL, compared with HA group (p < 0,05). Within the same time point, the gene expression for RUNX2 did not differ between the groups. Overall, data demonstrated that the HA hydrogels incorporating PL increased the cellular metabolism and stimulate the mineralized matrix deposition by hDPSCs, providing clear evidence of the potential of the proposed system for the repair of damaged pulp/dentin tissue and endodontics regeneration.

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Section: Production Of Methacrylated Hyaluronic Acidmentioning

confidence: 99%

Hyaluronic acid hydrogels incorporating platelet lysate enhance human pulp cell proliferation and differentiation

Almeida

Babo

Silva

et al. 2018

J Mater Sci: Mater Med

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“…25 (c) In the area of ''magnetic hydrogels'' numerous polymers have been designed 26 including magnetic hydrogel materials designed to be magnetically localized and those that are designed to undergo magnetically activated swelling, aggregation or degradation with the release of growth factors. 27 (d) The broad difference in pH between the stomach and other parts of the gastrointestinal (GI) tract allows pH-sensitive drug delivery systems to selectively deliver drugs. For example, the targeting of specific disease sites in the GI tract for local drug release or uptake/delivery of the drug via, for example, the small intestine.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable pH-responsive hydrogels for controlled dual-drug release

Qiu

Yang

et al. 2018

J. Mater. Chem. B

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“…Both hTDCs and hADSCs were viable within this construct. Interestingly, under magnetic field stimulation, the magnetic hydrogel showed degradation, and the cellular response of hADSCs on bonemimicking units and hTDCs on tendon-mimicking units was modulated (Silva et al, 2018). Later on, a new gradient material was designed by incorporating continuous gradients of BMP-2 loaded glycosylated MNPs into agarose hydrogels with an external magnetic field via the grafting-onto method.…”

Section: Applications In Bone Tissue Engineeringmentioning

confidence: 99%

“…Recently, magnetically responsive hydrogel, as one kind of smart hydrogels, has been introduced into biomedical applications in improving the biological activities of cells, tissues, or organs. This is mainly attributed to its magnetic responsiveness to external magnetic field and obtaining functional structures to remotely regulate physical, biochemical, and mechanical properties of the milieu surrounding the cells, tissues, or organs (Abdeen et al, 2016;Antman-Passig and Shefi, 2016;Rodkate and Rutnakornpituk, 2016;Bannerman et al, 2017;Omidinia-Anarkoli et al, 2017;Xie et al, 2017;Silva et al, 2018;Tay et al, 2018;Wang et al, 2018;Bowser and Moore, 2019;Ceylan et al, 2019;Luo et al, 2019). Recent studies have represented that magnetic hydrogel could act as an excellent drug release and targeting system.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances on Magnetic Sensitive Hydrogels in Tissue Engineering

et al. 2020

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Tissue engineering is a promising strategy for the repair and regeneration of damaged tissues or organs. Biomaterials are one of the most important components in tissue engineering. Recently, magnetic hydrogels, which are fabricated using iron oxide-based particles and different types of hydrogel matrices, are becoming more and more attractive in biomedical applications by taking advantage of their biocompatibility, controlled architectures, and smart response to magnetic field remotely. In this literature review, the aim is to summarize the current development of magnetically sensitive smart hydrogels in tissue engineering, which is of great importance but has not yet been comprehensively viewed.

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Multifunctional magnetic-responsive hydrogels to engineer tendon-to-bone interface

Cited by 79 publications

References 100 publications

Hyaluronic acid hydrogels incorporating platelet lysate enhance human pulp cell proliferation and differentiation

Hyaluronic acid hydrogels incorporating platelet lysate enhance human pulp cell proliferation and differentiation

Biodegradable pH-responsive hydrogels for controlled dual-drug release

Recent Advances on Magnetic Sensitive Hydrogels in Tissue Engineering

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