Multifunctional Photosensitizer-Based Contrast Agents for Photoacoustic Imaging

Ho, Chris Jun Hui; Balasundaram, Ghayathri; Driessen, Wouter H. P.; McLaren, Ross; Wong, Chi Lok; Dinish, U. S.; Attia, Amalina Binte Ebrahim; Ntziachristos, Vasilis; Olivo, Malini

doi:10.1038/srep05342

Cited by 112 publications

(106 citation statements)

References 32 publications

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“…33 From the PA signal collected from the abdomen of the mice, maximum intensity projection images were obtained ( Figure 4B-E) and ROI analyses were performed on individual organs such as liver, spleen, kidney, and intestines. Figure 5A and B illustrate that the PA signal from the probe could be detected in the liver and spleen immediately after the injection of both folate-CP dots and CP dots and for up to a week.…”

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confidence: 99%

Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer

Balasundaram¹,

Ho²,

Li³

et al. 2015

IJN

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Conjugated polymers (CPs) are upcoming optical contrast agents in view of their unique optical properties and versatile synthetic chemistry. Biofunctionalization of these polymer-based nanoparticles enables molecular imaging of biological processes. In this work, we propose the concept of using a biofunctionalized CP for noninvasive photoacoustic (PA) molecular imaging of breast cancer. In particular, after verifying the PA activity of a CP nanoparticle (CP dots) in phantoms and the targeting efficacy of a folate-functionalized version of the same (folate-CP dots) in vitro, we systemically administered the probe into a folate receptor-positive (FR+ve) MCF-7 breast cancer xenograft model to demonstrate the possible application of folate-CP dots for imaging FR+ve breast cancers in comparison to CP dots with no folate moieties. We observed a strong PA signal at the tumor site of folate-CP dots-administered mice as early as 1 hour after administration as a result of the active targeting of the folate-CP dots to the FR+ve tumor cells but a weak PA signal at the tumor site of CP-dots-administered mice as a result of the passive accumulation of the probe by enhanced permeability and retention effect. We also observed that folate-CP dots produced ~4-fold enhancement in the PA signal in the tumor, when compared to CP dots. These observations demonstrate the great potential of this active-targeting CP to be used as a contrast agent for molecular PA diagnostic imaging in various biomedical applications.

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confidence: 99%

Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer

Balasundaram¹,

Ho²,

Li³

et al. 2015

IJN

Self Cite

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“…Using optical imaging for the indication of drug distribution needs to be carefully discerned, especially when imaging agents are physically integrated with the drug. Adopting new imaging modalities, such as fluorescence lifetime [66] and photoacoustic techniques [67,68], may further the application of hybrid nanocrystals for concurrent anticancer therapy and bioimaging.…”

Section: Hybrid Nanocrystalsmentioning

confidence: 99%

Developing Nanocrystals for Cancer Treatment

Lü

Cao

Gemeinhart

et al. 2015

Nanomedicine (Lond.)

113

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Nanocrystals are carrier-free solid drug particles that are sized in the nanometer range and have crystalline characteristics. Due to high drug loading (as high as 100%) - free of organic solvents or solubilizing chemicals - nanocrystals have become attractive in the field of drug delivery for cancer treatment. Top-down and bottom-up approaches have been developed for preparing anticancer nanocrystals. In this review, preparation methods and in vivo performance of anticancer nanocrystals are discussed first, followed by an introduction of hybrid nanocrystals in cancer theranostics.

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“…34 Due to its high optical absorption properties compared to endogenous chromophores in the body, PS are also good photoacoustic contrast agents. 89 Recently we demonstrated that PAI can map the heterogeneous accumulation of methylene blue (optical absorption peak around 670 nm) loaded liposomes in brain tumors to aid in personalized PDT dosimetry. 90 As active PDT agents, a concern is that PS could be photobleached during imaging procedures.…”

Section: Introductionmentioning

confidence: 99%

Optical Imaging, Photodynamic Therapy and Optically Triggered Combination Treatments

et al. 2015

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Optical imaging is becoming increasingly promising for real-time image-guided resections and combined with photodynamic therapy (PDT), a photochemistry-based treatment modality, optical approaches can be intrinsically “theranostic”. Challenges in PDT include precise light delivery, dosimetry and photosensitizer tumor localization to establish tumor selectivity, and like all other modalities, incomplete treatment and subsequent activation of molecular escape pathways are often attributable to tumor heterogeneity. Key advances in molecular imaging, target-activatable photosensitizers and optically active nanoparticles that provide both cytotoxicity and a drug release mechanism, have opened exciting avenues to meet these challenges. The focus of the review is optical imaging in the context of PDT but the general principles presented are applicable to many of the conventional approaches to cancer management. We highlight the role of optical imaging in providing structural, functional and molecular information regarding photodynamic mechanisms of action, thereby advancing PDT and PDT-based combination therapies of cancer. These advances represent a PDT renaissance with increasing applications of clinical PDT as a frontline cancer therapy working in concert with fluorescence-guided surgery, chemotherapy and radiation.

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Multifunctional Photosensitizer-Based Contrast Agents for Photoacoustic Imaging

Cited by 112 publications

References 32 publications

Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer

Molecular photoacoustic imaging of breast cancer using an actively targeted conjugated polymer

Developing Nanocrystals for Cancer Treatment

Optical Imaging, Photodynamic Therapy and Optically Triggered Combination Treatments

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