Multifunctional properties of organic-inorganic hybrid nanocomposites based on chitosan derivatives and layered double hydroxides for ocular drug delivery

Xu, Tingting; Zhang, Jie; Chi, Huibo; Cao, Feng

doi:10.1016/j.actbio.2016.02.041

Cited by 60 publications

(39 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…19 Briefly, PRN (140 mg, 0.4 mmol) was dissolved in 70 mL of deionized water and different amounts of CG-GS (37.5, 50 …”

Section: 30mentioning

confidence: 99%

“…19 Briefly, 50 μL of CG-GS-PRN-LDH hybrid nanocomposite eye drops containing 2.5 μg of PRN were added in two …”

Section: In Vivo Tissue Distribution Study In Rabbitsmentioning

confidence: 99%

“…The solid precipitation was filtered to get the target product as white solid (2.27 g CTS-Fmoc-GS was synthesized by amide reaction between carboxylic acid groups of Fmoc-GS and amine groups of CTS. 19 Briefly, CTS (0.6 g) was hydrated in 3 mL of 1 M HCl and then dissolved in 35 mL of distilled water. The pH of the solution was adjusted to 5.0 with 1 M NaOH.…”

Section: Synthesis and Characterization Of Cg-gs Synthesis Of Cg-gsmentioning

confidence: 99%

“…19 Briefly, artificial tear (6.78 g/L NaCl, 2.18 g/L NaHCO 3 , 1.38 g/L KCl, 0.084 g/L CaCl 2 •2H 2 O, pH 7.4) was employed as the release medium. Samples containing 10 mg PRN were suspended in 900 mL of artificial tear and incubated at 35°C±0.5°C with the speed at 100 rpm.…”

Section: In Vitro Release Studymentioning

confidence: 99%

“…18 The multifunctional nanocomposites with target ligand of dipeptide VV have been demonstrated with active targeting of PepT-1 in animals and at the cellular level. 19,20 These intercalated nanocomposites with a synergistic effect between the organic and inorganic materials have received more and more attention in recent years. [21][22][23] Glycylsarcosine (GS) is one of the best known reference substrates of PepT-1 and commonly used as an inhibitor in the study of transport mechanism of PepT-1.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Functional intercalated nanocomposites with chitosan-glutathione-glycylsarcosine and layered double hydroxides for topical ocular drug delivery

Xu¹,

Xu²,

Gu³

et al. 2018

IJN

Self Cite

View full text Add to dashboard Cite

Background To enhance ocular bioavailability, the traditional strategies have focused on prolonging precorneal retention and improving corneal permeability by nano-carriers with positive charge, thiolated polymer, absorption enhancer and so on. Glycylsarcosine (GS) as an active target ligand of the peptide tranpsporter-1 (PepT-1), could specific interact with the PepT-1 on the cornea and guide the nanoparticles to the treating site. Purpose The objective of the study was to explore the active targeting intercalated nanocomposites based on chitosan-glutathione-glycylsarcosine (CG-GS) and layered double hydroxides (LDH) as novel carriers for the treatment of mid-posterior diseases. Materials and methods CG-GS-LDH intercalated nanocomposites were prepared by the coprecipitation hydrothermal method. In vivo precorneal retention study, ex vivo fluorescence images, in vivo experiment for distribution and irritation were studied in rabbits. The cytotoxicity and cellular uptake were studied in human corneal epithelial primary cells (HCEpiC). Results CG-GS-LDH nanocomposites were prepared successfully and characterized by FTIR and XRD. Experiments with rabbits showed longer precorneal retention and higher distribution of fluorescence probe/model drug. In vitro cytological study, CG-GS-LDH nanocomposites exhibited enhanced cellular uptake compared to pure drug solution. Furthermore, the investigation of cellular uptake mechanisms demonstrated that both the active transport by PepT-1 and clathrin-mediated endocytosis were involved in the internalization of CG-GS-LDH intercalated nanocomposites. An ocular irritation study and a cytotoxicity test indicated that these nanocomposites produced no significant irritant effects. Conclusions The active targeting intercalated nanocomposites could have great potential for topical ocular drug delivery due to the capacity for prolonging the retention on the ocular surface, enhancing the drug permeability through the cornea, and efficiently delivering the drug to the targeted site.

show abstract

“…19 Briefly, PRN (140 mg, 0.4 mmol) was dissolved in 70 mL of deionized water and different amounts of CG-GS (37.5, 50 …”

Section: 30mentioning

confidence: 99%

“…19 Briefly, 50 μL of CG-GS-PRN-LDH hybrid nanocomposite eye drops containing 2.5 μg of PRN were added in two …”

Section: In Vivo Tissue Distribution Study In Rabbitsmentioning

confidence: 99%

Section: Synthesis and Characterization Of Cg-gs Synthesis Of Cg-gsmentioning

confidence: 99%

Section: In Vitro Release Studymentioning

confidence: 99%