Multikingdom and functional gut microbiota markers for autism spectrum disorder

Su, Qi; Wong, Oscar W. H.; Lu, Wenqi; Wan, Yating; Zhang, Lin; Xu, Wenye; Li, Moses K. T.; Liu, Chengyu; Cheung, Chun Pan; Ching, Jessica Y. L.; Cheong, Pui Kuan; Leung, Ting Fan; Chan, Sandra; Leung, Patrick; Chan, Francis K. L.; Ng, Siew C.

doi:10.1038/s41564-024-01739-1

Cited by 4 publications

References 76 publications

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Human untargeted metabolomics in the gut microbiome era: ethanol vs methanol

Zuffa,

Charron-Lamoureux,

Brennan

et al. 2024

Preprint

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Untargeted metabolomics is frequently performed on human fecal samples in conjunction with sequencing to unravel gut microbiome functionality. As sample collection efforts rapidly expand, with individuals often collecting specimens at home, metabolomics experiments should adapt to accommodate the safety and needs of bulk off-site collections. Here, we show that a 95% ethanol, safe to be shipped and handled, extraction pipeline recovers comparable amounts of metabolites as a validated 50% methanol extraction, preserving metabolic profiles differences between subjects. Additionally, the fecal metabolome remains relatively stable when stored in 95% ethanol for up to a week at room temperature. Finally, we suggest a metabolomics data analysis workflow using robust centered log ratio transformation, which removes variance introduced by different sample weights, allowing for reliable and integration-ready untargeted metabolomics experiments in gut microbiome studies.

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Human untargeted metabolomics in the gut microbiome era: ethanol vs methanol

Zuffa,

Charron-Lamoureux,

Brennan

et al. 2024

Preprint

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Gut MicrobiotAware: how much do we know about gut microbiota? An international questionnaire

Garcia-Gutierrez,

Arbulu,

Oliver

et al. 2024

Preprint

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Over the past two decades, our understanding of human gut microbiota composition and function has grown significantly. The surge in studies and data has unveiled the profound influence of gut microbiota on host nutrition, health, and behavior, bridging biology, medicine, and ecology. The dynamic interaction between daily choices, life events, and gut microbiota composition makes it a fascinating subject for scientists to communicate to diverse audiences. Effective scientific communication involves adapting the delivery of knowledge to different audiences, using precise language in academic settings and accessible concepts in public forums like science festivals or social media. With the growing interest in gut microbiota beyond academic circles, there is an increased risk of disseminating information lacking scientific rigor. This study aims to assess the general public's knowledge about gut microbiota and evaluate healthcare professionals' understanding of its links to various health conditions, ultimately informing better communication strategies for both groups.

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Exploring the Frontier: The Human Microbiome’s Role in Rare Childhood Neurological Diseases and Epilepsy

Belnap,

Ramsey,

Carvalho

et al. 2024

Brain Sciences

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Emerging research into the human microbiome, an intricate ecosystem of microorganisms residing in and on our bodies, reveals that it plays a pivotal role in maintaining our health, highlighting the potential for microbiome-based interventions to prevent, diagnose, treat, and manage a myriad of diseases. The objective of this review is to highlight the importance of microbiome studies in enhancing our understanding of rare genetic epilepsy and related neurological disorders. Studies suggest that the gut microbiome, acting through the gut–brain axis, impacts the development and severity of epileptic conditions in children. Disruptions in microbial composition can affect neurotransmitter systems, inflammatory responses, and immune regulation, which are all critical factors in the pathogenesis of epilepsy. This growing body of evidence points to the potential of microbiome-targeted therapies, such as probiotics or dietary modifications, as innovative approaches to managing epilepsy. By harnessing the power of the microbiome, we stand to develop more effective and personalized treatment strategies for children affected by this disease and other rare neurological diseases.

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Multikingdom and functional gut microbiota markers for autism spectrum disorder

Cited by 4 publications

References 76 publications

Human untargeted metabolomics in the gut microbiome era: ethanol vs methanol

Human untargeted metabolomics in the gut microbiome era: ethanol vs methanol

Gut MicrobiotAware: how much do we know about gut microbiota? An international questionnaire

Exploring the Frontier: The Human Microbiome’s Role in Rare Childhood Neurological Diseases and Epilepsy

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