Multilayer adsorption of lectins on glycosylated microporous polypropylene membranes

Glycopolymer Brushes for the Affinity Adsorption of RCA120: Effects of Thickness, Grafting Density, and Epitope Density

“…Since the binding capacity largely depends on the amounts of affinity ligands, not implausibly, polymer chains bound with large amounts of ligands are better than small molecule ligands for high-performance affinity membrane chromatography. Grafting polymerization is usually a powerful tool for introducing polymeric ligands to the membrane surfaces. − By using monomer with sugar pendant, glycopolymer ligands have been successfully employed onto the membrane surfaces for the affinity adsorption of lectins. − In these cases, the grafting polymer layer will generally form brush structure with certain grafting density . It is reported that the amount of bound lectins is greatly affected by the grafting density and the chain composition of the glycopolymer brushes. − At the same time, these brushes show very low nonspecific adsorption to biofoulants due to their superhydrophilicity and charge neutrality. − However, it is still unclear about the effect of comprehensive architectures of glycopolymer brushes, such as thickness, grafting density, and epitope density, on affinity binding of lectins.…”

Membrane Chromatography and Fractionation of Proteins from Whey—A Review

“…Membrane functionalization is an important step in altering the surface potential and charge of a membrane in a significant way without changing too much of the membrane's primary structure [80]. It is understandable that membrane/modified membranes are required to sustain function in potential harsh conditions, including a wide range of temperatures, pH levels, and ionic strengths during chromatographic processes [63,74,78,81].…”