Multilayered tissue mimicking skin and vessel phantoms with tunable mechanical, optical, and acoustic properties

Chen, Alvin I.; Balter, Max L.; Chen, Melanie I.; Groß, Daniel; Alam, S. Kaisar; Maguire, Timothy J.; Yarmush, Martin L.

doi:10.1118/1.4951729

Cited by 117 publications

(102 citation statements)

References 83 publications

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“…Previously, we described the mechanical design of a robotic venipuncture device [11,12] (Fig. 5a) and demonstrated early results in multilayered gelatin-based phantoms simulating the mechanical, optical, and ultrasonic properties of human skin, vessels, and blood [13]. However, the full algorithmic framework had not been implemented, and the performance of the robot was not compared to manual techniques.…”

Section: Resultsmentioning

confidence: 99%

3D Near Infrared and Ultrasound Imaging of Peripheral Blood Vessels for Real-Time Localization and Needle Guidance

Chen

Balter

Maguire

et al. 2016

Lecture Notes in Computer Science

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This paper presents a portable imaging device designed to detect peripheral blood vessels for cannula insertion that are otherwise difficult to visualize beneath the skin. The device combines near infrared stereo vision, ultrasound, and real-time image analysis to map the 3D structure of subcutaneous vessels. We show that the device can identify adult forearm vessels and be used to guide manual insertions in tissue phantoms with increased first-stick accuracy compared to unassisted cannulation. We also demonstrate that the system may be coupled with a robotic manipulator to perform automated, image-guided venipuncture.

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Section: Resultsmentioning

confidence: 99%

3D Near Infrared and Ultrasound Imaging of Peripheral Blood Vessels for Real-Time Localization and Needle Guidance

Chen

Balter

Maguire

et al. 2016

Lecture Notes in Computer Science

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“…4), where a successful venipuncture was defined as placing the needle in the center of the surrogate vessel, and collection of 4 ml of fluid. Phantoms were fabricated based on the protocol described in [15], and extended by perfusing a viscous solution through the vessels. Briefly, phantoms contained two different sized veins (∅3 and 2 mm) composed of silicone tubing (Silastic silicone, Dow-Corning) embedded in a 10% gelatin matrix.…”

Section: Experimental Methods and Resultsmentioning

confidence: 99%

System design and development of a robotic device for automated venipuncture and diagnostic blood cell analysis

Balter

Chen

Fromholtz

et al. 2016

2016 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS)

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Diagnostic blood testing is the most prevalent medical procedure performed in the world and forms the cornerstone of modern health care delivery. Yet blood tests are still predominantly carried out in centralized labs using large-volume samples acquired by manual venipuncture, and no end-to-end solution from blood draw to sample analysis exists today. Our group is developing a platform device that merges robotic phlebotomy with automated diagnostics to rapidly deliver patient information at the site of the blood draw. The system couples an image-guided venipuncture robot, designed to address the challenges of routine venous access, with a centrifuge-based blood analyzer to obtain quantitative measurements of hematology. In this paper, we first present the system design and architecture of the integrated device. We then perform a series of in vitro experiments to evaluate the cannulation accuracy of the system on blood vessel phantoms. Next, we assess the effects of vessel diameter, needle gauge, flow rate, and viscosity on the rate of sample collection. Finally, we demonstrate proof-of-concept of a white cell assay on the blood analyzer using in vitro human samples spiked with fluorescently labeled microbeads.

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“…Agar is a heterogeneous mixture of two classes of polysaccharide polymer derived from seaweed and used to cast gel layers in skin phantom model slabs . Baseline SWIR imaging was performed on agar slabs.…”

Section: Methodsmentioning

confidence: 99%

“…Agar is a heterogeneous mixture of two classes of polysaccharide polymer derived from seaweed and used to cast gel layers in skin phantom model slabs. 16 Baseline SWIR imaging was performed on agar slabs. Slabs using 1 g agar (Sigma Aldrich Corporation, St. Louis, MO) mixed into 30 mL water were constructed in petri dishes (ThermoFisher Scientific, Waltham, MA) as 2D layers with 1, 2, 3, 5, and 10 mm thickness.…”

Section: Agar Preparation For Baseline Swir Characteristicsmentioning

confidence: 99%

Short‐wave infrared light imaging measures tissue moisture and distinguishes superficial from deep burns

Mironov

Hwang

Nemzek

et al. 2019

Wound Repair Regeneration

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Existing clinical approaches and tools to measure burn tissue destruction are limited resulting in misdiagnosis of injury depth in over 40% of cases. Thus, our objective in this study was to characterize the ability of short‐wave infrared (SWIR) imaging to detect moisture levels as a surrogate for tissue viability with resolution to differentiate between burns of various depths. To accomplish our aim, we constructed an imaging system consisting of a broad‐band Tungsten light source; 1,200‐, 1,650‐, 1,940‐, and 2,250‐nm wavelength filters; and a specialized SWIR camera. We initially used agar slabs to provide a baseline spectrum for SWIR light imaging and demonstrated the differential absorbance at the multiple wavelengths, with 1,940 nm being the highest absorbed wavelength. These spectral bands were then demonstrated to detect levels of moisture in inorganic and in vivo mice models. The multiwavelength SWIR imaging approach was used to diagnose depth of burns using an in vivo porcine burn model. Healthy and injured skin regions were imaged 72 hours after short (20 seconds) and long (60 seconds) burn application, and biopsies were extracted from those regions for histologic analysis. Burn depth analysis based on collagen coagulation histology confirmed the formation of superficial and deep burns. SWIR multispectral reflectance imaging showed enhanced intensity levels in long burned regions, which correlated with histology and distinguished between superficial and deep burns. This SWIR imaging method represents a novel, real‐time method to objectively distinguishing superficial from deep burns.

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Multilayered tissue mimicking skin and vessel phantoms with tunable mechanical, optical, and acoustic properties

Abstract: The phantoms described in this work simulate the mechanical, optical, and acoustic properties of human skin tissues, vessel tissue, and blood. In this way, the phantoms are uniquely suited to serve as test models for multimodal imaging techniques and image-guided interventions.

Cited by 117 publications

References 83 publications

3D Near Infrared and Ultrasound Imaging of Peripheral Blood Vessels for Real-Time Localization and Needle Guidance

3D Near Infrared and Ultrasound Imaging of Peripheral Blood Vessels for Real-Time Localization and Needle Guidance

System design and development of a robotic device for automated venipuncture and diagnostic blood cell analysis

Short‐wave infrared light imaging measures tissue moisture and distinguishes superficial from deep burns

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