Multilineage Potential of Homozygous Stem Cells Derived from Metaphase II Oocytes

Lin, Helen; Lei, Jingqi; Wininger, David; Nguyen, Minh‐Thanh; Khanna, Ruchi; Hartmann, Chris; Yan, Wen‐Liang; Huang, Shun

doi:10.1634/stemcells.21-2-152

Cited by 84 publications

(60 citation statements)

References 17 publications

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“…Several previous studies have reported the successful activation of human oocytes and the formation of parthenogenetic blastocysts thereafter [10][11][12], but the successful derivation of parthenogenetic hESCs (pESCs) was reported only recently [13], owing to the inaccessibility of human oocytes and the existing ethical concerns about using viable fresh human oocytes in the process [14].…”

Section: Introductionmentioning

confidence: 99%

A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure

et al. 2007

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Homozygous human embryonic stem cells (hESCs) are thought to be better cell sources for hESC banking because their human leukocyte antigen (HLA) haplotype would strongly increase the degree of matching for certain populations with relatively smaller cohorts of cell lines. Homozygous hESCs can be generated from parthenogenetic embryos, but only heterozygous hESCs have been established using the current strategy to artificially activate the oocyte without second polar body extrusion. Here we report the first successful derivation of a human homozygous ESC line (chHES-32) from a one-pronuclear oocyte following routine in vitro fertilization treatment. chHES-32 cells express common markers and genes with normal hESCs. They have been propagated in an undifferentiated state for more than a year (>P50) and have maintained a stable karyotype of 46, XX. When differentiated in vivo and in vitro, chHES-32 cells can form derivatives from all three embryonic germ layers. The almost undetectable expression of five paternally expressed imprinted genes and their HLA genotype identical to the oocyte donor indicated their parthenogenetic origin. Using genome-wide single-nucleotide polymorphism analysis and DNA fingerprinting, the homozygosity of chHES-32 cells was further confirmed. The results indicated that 'unwanted' one-pronuclear oocytes might be a potential source for human homozygous and parthenogenetic ESCs, and suggested an alternative strategy for obtaining homozygous hESC lines from parthenogenetic haploid oocytes.

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Section: Introductionmentioning

confidence: 99%

A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure

et al. 2007

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“…Recently, Kim et al [5] demonstrated that selected pES cells can serve as a source of histocompatible tissues for transplantation. Although both mouse and primate pES cells have been demonstrated to undergo extensive differentiation in vitro [6,7], and to contribute to a variety of adult tissues in chimeric mice [8], there are some discrepancies regarding the proliferation capacities of pES cells [7,[9][10][11]. Therefore, it is necessary to fully explore the developmental potential of pES cells both in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

Activation of paternally expressed imprinted genes in newly derived germline-competent mouse parthenogenetic embryonic stem cell lines

et al. 2007

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“…Telomere shortening eventually causes chromosomal instability, leading to the activation of DNA damage response pathway followed by p53-dependent cell cycle arrest, senescence, and cell death (Vaziri and Benchimol, 1996). Telomerase was found to be activated in embryonic germ cells and embryonic stem cells, repressed in normal somatic cells, and reactivated in a large majority of tumor cells (Liu, 2000;Pera et al, 2000;Lin et al, 2003). Both the parental clone (Rat-A2B2) (Young and Black, 2004;Young et al, 2004) and its transfected progeny Scl-40␤ (Fig.…”

Section: Similarities Between Embryonic Stem Cells and Adult Ppelscsmentioning

confidence: 99%

“…Implantedtransplanted embryonic stem cells form teratomas (Thomson et al, 1998;Amit et al, 2000;Pera et al, 2000;Reubinoff et al, 2000;Lin et al, 2003;Watkitani et al, 2003). In contrast, undifferentiated adult pluripotent stem cells incorporate into all tissues in need of repair.…”

Section: Differences Between Embryonic Stem Cells and Adult Ppelscsmentioning

confidence: 99%

“…After isolation and growth in vitro with inhibitory agents (i.e., leukemia inhibitory factor, ESGRO, and/or fibroblast feeder layers), these cells exhibit immunological and molecular markers for undifferentiated embryonic cells (Niwa et al, 2000;Pera et al, 2000;Pesce and Scholer, 2001;Henderson et al, 2002;Cheng et al, 2003). They exhibit telomerase activity, which is consistent with their extended capability for self-renewal (Liu, 2000;Pera et al, 2000;Lin et al, 2003). When released from inhibitory control in vitro, these cells will spontaneously differentiate into and exhibit phenotypic expression markers for cells of ectodermal, mesodermal, and endodermal origin (Thomson et al, 1995(Thomson et al, , 1998Shamblott et al, 1998;Pera et al, 2000).…”

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confidence: 96%

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Clonogenic analysis reveals reserve stem cells in postnatal mammals. II. Pluripotent epiblastic‐like stem cells

et al. 2004

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Undifferentiated cells have been identified in the prenatal blastocyst, inner cell mass, and gonadal ridges of rodents and primates, including humans. After isolation these cells express molecular and immunological markers for embryonic cells, capabilities for extended self‐renewal, and telomerase activity. When allowed to differentiate, embryonic stem cells express phenotypic markers for tissues of ectodermal, mesodermal, and endodermal origin. When implanted in vivo, undifferentiated noninduced embryonic stem cells formed teratomas. In this report we describe a cell clone isolated from postnatal rat skeletal muscle and derived by repetitive single‐cell clonogenic analysis. In the undifferentiated state it consists of very small cells having a high ratio of nucleus to cytoplasm. The clone expresses molecular and immunological markers for embryonic stem cells. It exhibits telomerase activity, which is consistent with its extended capability for self‐renewal. When induced to differentiate, it expressed phenotypic markers for tissues of ectodermal, mesodermal, and endodermal origin. The clone was designated as a postnatal pluripotent epiblastic‐like stem cell (PPELSC). The undifferentiated clone was transfected with a genomic marker and assayed for alterations in stem cell characteristics. No alterations were noted. The labeled clone, when implanted into heart after injury, incorporated into myocardial tissues undergoing repair. The labeled clone was subjected to directed lineage induction in vitro, resulting in the formation of islet‐like structures (ILSs) that secreted insulin in response to a glucose challenge. This study suggests that embryonic‐like stem cells are retained within postnatal mammals and have the potential for use in gene therapy and tissue engineering. Anat Rec Part A 277A:178–203, 2004. © 2004 Wiley‐Liss, Inc.

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Multilineage Potential of Homozygous Stem Cells Derived from Metaphase II Oocytes

Abstract: ABSTRACT

Cited by 84 publications

References 17 publications

A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure

A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure

Activation of paternally expressed imprinted genes in newly derived germline-competent mouse parthenogenetic embryonic stem cell lines

Clonogenic analysis reveals reserve stem cells in postnatal mammals. II. Pluripotent epiblastic‐like stem cells

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