Multilocular adipocytes from muscovy ducklings differentiated in response to cold acclimation.

Barré, Hervé; Cohen-Adad, Frédérique; Duchamp, Claude; Rouanet, Jean‐Louis

doi:10.1113/jphysiol.1986.sp016103

Cited by 79 publications

(43 citation statements)

References 32 publications

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“…Hence, skeletal muscle NST would rather be sustained either by increasing ATP breakdown through the futile cycling of calcium across the sarcoplasmic reticulum membrane, for example (Dumonteil et al, 1995), or by decreasing mitochondrial ATP efficiency through the oxidation of FADH 2 -linked substrates such as fatty acyl-carnitine (Clerc et al, 2007). The latter hypothesis would link avUCP gene expression with the mobilization of fat reserves and the increased potential of skeletal muscle to oxidize fatty acid when birds are exposed to low temperatures (Barré et al, 1986;Benistant et al, 1998;Bedu et al, 2002). Further insight, avUCP can also have a role in the control of radical oxidative species production ( al., 2010b) when skeletal muscle mitochondria oxidize fatty acids Seifert et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…In the absence of both mammalian thermogenic brown adipose tissue and UCP1 (UCP, uncoupling protein) in birds (Johnston, 1971;Barré et al, 1986), there is some evidence that mitochondrial loose coupling might contribute to cold-induced heat production in skeletal muscle (Skulachev and Maslov, 1960;Duchamp et al, 1992;Roussel et al, 1998), i.e. the major tissue contributing to 70% of the observed NST in cold-acclimated ducklings (Duchamp and Barré, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Cold-acclimation-induced non-shivering thermogenesis in birds is associated with upregulation of avian UCP but not with innate uncoupling or altered ATP efficiency

Teulier

Rouanet

Letexier

et al. 2010

Journal of Experimental Biology

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) were obtained from a commercial stockbreeder (Eclosion Grimaud-lacorbière, Roussay, France). Birds were fed ad libitum with commercial mash (Moulin Guenard, 645000MI, Vonnas, France) and had free access to water. From the age of 10 days, ducklings were caged for Accepted 7 April 2010 SUMMARY Despite their lack of brown adipose tissue, some bird species develop regulatory non-shivering thermogenesis (NST) of skeletal muscle origin in response to cold acclimation. Mechanisms involved in avian NST are still unclear but may involve reduced energetic coupling in skeletal muscle mitochondria through the expression of an avian homologue of mammalian uncoupling proteins. The aim of this work was to investigate whether the expression of avian uncoupling protein (avUCP) would correlate with the capacity for cold-induced muscle NST. Various levels of cold acclimation were obtained by rearing 1-week-old ducklings (Cairina moschata) for 4 weeks at three different ambient temperatures (25°C, 11°C or 4°C). Muscle NST was measured by simultaneous recordings of metabolic rate and electromyographic activity (gastrocnemius muscle) at ambient temperatures (T a ) ranging from 27°C to -5°C. The expression of avUCP gene and mitochondrial bioenergetics were also determined in gastrocnemius muscle. Results showed that muscle NST capacity depends on the T a at which ducklings were acclimated, i.e. the lower the rearing temperature, the higher the capacity for NST. This increased metabolic heat production occurred in parallel with an upregulation of avUCP, which was not associated with a change in mitochondrial membrane conductance. The intensity of mitochondrial oxidative phosphorylation also increased in proportion with the harshness of cold, while the efficiency of ATP generation was equally effective in all three acclimation temperatures. In the absence of mitochondrial uncoupling, these data indicate a clear link between avUCP expression and the capacity of ducklings to adjust their muscular aerobic activity to cold exposure.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cold-acclimation-induced non-shivering thermogenesis in birds is associated with upregulation of avian UCP but not with innate uncoupling or altered ATP efficiency

Teulier

Rouanet

Letexier

et al. 2010

Journal of Experimental Biology

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“…For the same body weight and egg production, the inefficient line consumes 30-40 % more food than the efficient line, is leaner [3], and shows a larger (j133 %) heat production after a meal [4]. Enhanced capacity for regulatory NST, as assessed by simultaneous measurements of heat production and electromyographic activity, was reported in response to long-term cold exposure in young ducklings and king-penguin (Aptenodytes patagonicus) chicks [5,6]. Avian NST was also induced by chronic treatment of ducklings kept at thermoneutrality with glucagon, a thermogenic hormone in birds [6].…”

Section: Introductionmentioning

confidence: 92%

“…From the age of 1 week, ducklings were caged in groups of six for a period of 4 weeks at 25 mC (thermoneutral controls) or exposed to 4 mC (coldacclimated animals) to induce the development of skeletal-muscle NST [5,7]. Another batch of ducklings received daily injections of glucagon (100 µg:kg −" , intraperitoneally twice daily) for 4 weeks starting from the age of 1 week.…”

Section: Figure 1 Predicted Amino Acid Sequence and Alignment Of Avucmentioning

confidence: 99%

An uncoupling protein homologue putatively involved in facultative muscle thermogenesis in birds

RAIMBAULT

Dridi²,

DENJEAN

et al. 2001

Biochemical Journal

143

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The cDNA of an uncoupling protein (UCP) homologue was obtained by screening a chicken skeletal-muscle library. The predicted 307-amino-acid sequence of avian UCP (avUCP) is 55, 70, 70 and 46% identical with mammalian UCP1, UCP2 and UCP3 and plant UCP respectively. avUCP mRNA expression is restricted to skeletal muscle and its abundance was increased 1.3-fold in a chicken line showing diet-induced thermogenesis, and 3.6- and 2.6-fold in cold-acclimated and glucagon-treated ducklings developing muscle non-shivering thermogenesis respectively. The present data support the implication of avUCP in avian energy expenditure.

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“…The question arises as to whether these changes are developed for shivering or for non-shivering thermogenesis. During the first days of cold exposure, young ducklings resort mainly to shivering for heat production, but when the cold exposure is prolonged, NST is developed (Barre et al 1986a). Therefore, shivering could play a part in morphological changes of skeletal muscle observed in cold-acclimated ducklings.…”

Section: Effect Of Glacagon Treatmentmentioning

confidence: 99%

Histochemical arguments for muscular non‐shivering thermogenesis in muscovy ducklings.

Duchamp

Cohen-Adad

Rouanet

et al. 1992

The Journal of Physiology

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SUMMARY1. The histochemical characteristics of gastrocnemius muscle were investigated in 6-week-old cold-acclimated (5 weeks, 4 TC) and glucagon-treated (5 weeks, 25 0C, 103 nmol/kg i.P. twice daily) muscovy ducklings, two groups able to develop nonshivering thermogenesis in vivo. A comparison was made with thermoneutral controls (25 'C) of the same age. All animals were fed ad libitum. Fibre type, fibre area and capillary supply have been studied. Further, a quantitative histochemical method for mitochondrial Mg2+-ATPase activity was developed to characterize the mitochondrial coupling state in situ.2. White gastrocnemius was composed of fast glycolytic (FG) and fast oxidative glycolytic (FOG) fibres, while red gastrocnemius contained FOG and slow oxidative (SO) fibres. In white gastrocnemius, the proportion of FG fibres was higher in glucagon-treated than in control or cold-acclimated ducklings. In red gastrocnemius, the proportion of SO fibres was higher in both cold-acclimated and glucagon-treated ducklings than in controls. The area of all fibres was generally lower in glucagontreated than in other ducklings.3. The capillary density was higher in both red and white components of the gastrocnemius muscle in cold-acclimated and glucagon-treated than in control ducklings, as a result of an increased number of capillaries around each fibre.4. In all fibres, except the FG type in cold-acclimated ducklings, the staining intensity of the Mg2+-ATPase reaction was higher in cold-acclimated and glucagontreated than in control ducklings whereas the staining intensity with maximal decoupling of oxidative phosphorylation by dinitrophenol was unchanged. This indicated a more loose-coupled state of mitochondria in situ in all fibres of coldacclimated ducklings, and in FOG fibres of white gastrocnemius and SO fibres of red gastrocnemius in glucagon-treated ducklings. C. DUCHAMP AND OTHERS 5. These results indicated a higher oxidative metabolism of skeletal muscle in both cold-acclimated and glucagon-treated than in control ducklings, and for most of the parameters studied, a similarity between cold acclimation and glucagon treatment. Because of the higher loose-coupled state of muscle mitochondria in cold-acclimated and glucagon-treated than in control ducklings, the higher oxidative capacity of skeletal muscle in these ducklings could be used for heat production rather than ATP synthesis and account for muscular non-shivering thermogenesis.

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Multilocular adipocytes from muscovy ducklings differentiated in response to cold acclimation.

Cited by 79 publications

References 32 publications

Cold-acclimation-induced non-shivering thermogenesis in birds is associated with upregulation of avian UCP but not with innate uncoupling or altered ATP efficiency

Cold-acclimation-induced non-shivering thermogenesis in birds is associated with upregulation of avian UCP but not with innate uncoupling or altered ATP efficiency

An uncoupling protein homologue putatively involved in facultative muscle thermogenesis in birds

Histochemical arguments for muscular non‐shivering thermogenesis in muscovy ducklings.

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