Multilocular hepatic masses due to Enterobius vermicularis

Riedel, J.; Halm, U.; Prause, C.; Vollrath, F.; Friedrich, N.; Weidel, A.; Wendt, Sebastian; Zachäus, M.

doi:10.1007/s00108-023-01479-0

Cited by 3 publications

(4 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The risk of zoonotic diseases is increased by the isolation of potentially harmful foodborne pathogens such as Brucella spp. and P. vulgaris [ 59 ], from apparently healthy abattoir livestock. Microbial contamination of the abattoir meat may occur during the exsanguination process, particularly if a sterile environment is not maintained [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Polyphasic Characterization of Brucella spp. in Livestock Slaughtered from Abattoirs in Eastern Cape, South Africa

Mazwi,

Kolo,

Jaja

et al. 2024

Microorganisms

View full text Add to dashboard Cite

In livestock, brucellosis is mainly an asymptomatic disease except when abortion occurs; therefore, two serological tests are used for diagnosis as no single test is suitable. Abattoir samples enable a combination of culture, molecular, and serological tests to detect brucellosis. This study assessed Brucella-specific PCR (ITS-PCR) to detect brucellosis and to conduct a molecular characterization of Brucella spp. isolated from PCR-positive livestock (n = 565) slaughtered at abattoirs and the appropriate sample tissue(s). ITS-PCR detected Brucella DNA in 33.6% of cattle, 14.5% of sheep, and 4.7% of pig tissues. Impure Brucella cultures from PCR-positive tissues were 43.6% (44/94) of cattle, 51.7% (15/29) of sheep, and 50% (2/4) of pigs with predominantly B. abortus identification with AMOS-PCR and low isolation of mixed B. abortus and B. melitensis in all species. In cattle, 33% of isolates were from lymph nodes, while in sheep 38.0% were from the liver and kidney and only from tonsils in pigs (2/4). Brucella infections identified with AMOS-PCR were present in seropositive and mainly seronegative (75.6–100%) livestock with the potential to cause brucellosis during pregnancy or breeding. This study demonstrated the value of the polyphasic approach, especially with chronic infections and the potential risk of these asymptomatic animals.

show abstract

Section: Discussionmentioning

confidence: 99%

Polyphasic Characterization of Brucella spp. in Livestock Slaughtered from Abattoirs in Eastern Cape, South Africa

Mazwi,

Kolo,

Jaja

et al. 2024

Microorganisms

View full text Add to dashboard Cite

show abstract

“…As this species is used for medicinal/magic purposes, or as pets, the presence of potentially pathogenic foodborne P . vulgaris highlights the risk of zoonotic infection [ 64 , 65 ]. The identification of Gram-negative bacteria such as P .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, apart from reptile-specific to low-pathogenic cestodes and innocuous fungi, P. vulgaris was identified from a Mediterranean chameleon in the souk. As this species is used for medicinal/ magic purposes, or as pets, the presence of potentially pathogenic foodborne P. vulgaris highlights the risk of zoonotic infection [64,65]. The identification of Gram-negative bacteria such as P. vulgaris in reptiles is a common finding in the cloaca, as reptiles are healthy carriers and spreaders of this potentially zoonotic pathogen [65].…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Snakes and Souks: Zoonotic pathogens associated to reptiles in the Marrakech markets, Morocco

Mendoza-Roldan,

Noll Louzada-Flores,

Lekouch

et al. 2023

PLoS Negl Trop Dis

View full text Add to dashboard Cite

The world-famous markets of Marrakech, also known in Arabic as souks, harbor a vast diversity of reptiles that are sold for medicinal/magic/pet purposes or used for snake charming. This unique epidemiological context has never been studied considering the interactions of humans, reptiles, and zoonotic pathogens. Thus, the aim of this study was to identify the parasites and pathogens present in blood and feces associated with handled reptiles in the markets of Marrakech to assess the risk of zoonotic transmission within the reptile-human interface. Privately owned reptiles (n = 118), coming from vendors or snake charmers, were examined and blood and feces sampled. DNA was extracted and molecular screening (cPCR, nPCR, qPCR, dqPCR) was performed aiming to identify potentially zoonotic pathogens (i.e., Anaplasma/Ehrlichia spp., Rickettsia spp., Borrelia burgdorferi sensu lato, Coxiella burnetii, Babesia/Theileria spp., Cryptosporidium spp., Giardia spp., Leishmania spp., Cestoda). Overall, 28.9% (34/118) of reptiles were positive for at least one pathogen. In blood, Anaplasma spp. were detected in four snakes, with two Montpellier snakes positive for Anaplasma phagocytophilum, while Rickettsia spp. were detected in one Mediterranean chameleon and four puff adders. Leishmania tarentolae was molecularly detected in a Mediterranean chameleon and a Montpellier snake. In feces, the cox1 gene generated a myriad of sequences for nematodes, cestodes, fungi and bacteria. Importantly, Proteus vulgaris was identified from a Mediterranean chameleon. Cryptosporidium spp. nPCR yielded a positive sample (i.e., Cryptosporidium sp. apodemus genotype I) from a Moroccan worm lizard, as well as for bacteria such as Pseudomonas aeruginosa in an Egyptian cobra, and Morganella morganii from a puff adder. Results from this study demonstrated the risk of zoonotic transmission of microorganisms and parasites present in blood and feces from reptiles that are brought to the souks in Marrakech, Morocco, to be sold for medicinal purposes or used for snake charming, being in direct and straight contact with humans.

show abstract

“…The risk of zoonotic disease is increased by the isolation of potentially harmful foodborne such as Brucella spp. and P. vulgaris [57], from apparently healthy abattoir livestock. Microbial contamination of the abattoir meat may occur during the exsanguination process, particularly if a sterile environment is not maintained [58].…”

Section: Cuktibacterium Acnes 3%mentioning

confidence: 99%

Polyphasic Characterization of Brucella spp. in Livestock Slaughtered from Abattoirs in Eastern Cape, South Africa

Mazwi,

Kolo,

Jaja

et al. 2023

Preprint

View full text Add to dashboard Cite

In livestock, brucellosis is mainly an asymptomatic disease except for abortion, therefore two serological tests are used for diagnosis as no single test is suitable. Abattoir samples enable a combination of culture, molecular and serological tests to detect brucellosis. This study assessed Brucella specific PCR (ITS-PCR) to detect brucellosis and to conduct molecular characterization of Brucella spp. isolated from PCR-positive livestock (n=565) slaughtered at abattoirs and the appropriate sample tissue(s). ITS-PCR detected Brucella DNA in 33.6% cattle, 14.5% sheep and 4.7% pig tissues. Impure Brucella cultures from PCR-positive tissues were 43.6% (44/94) cattle, 51.7% (15/29) sheep, and 50% (2/4) pigs with predominantly B. abortus identification with AMOS-PCR and low isolation of mixed B. abortus and B. melitensis in all species. In cattle 33% of isolates were from lymph nodes while in sheep 38.0% were from liver and kidney and only from tonsils in pigs (2/4). Brucella infections identified with AMOS-PCR were present in seropositive and mainly seronegative (75.6-100%) livestock with the potential to cause brucellosis during pregnancy or breeding. This study demonstrated the value of the polyphasic approach, especially with chronic infections and the potential risk of these asymptomatic animals.

show abstract

Multilocular hepatic masses due to Enterobius vermicularis

Cited by 3 publications

References 9 publications

Polyphasic Characterization of Brucella spp. in Livestock Slaughtered from Abattoirs in Eastern Cape, South Africa

Polyphasic Characterization of Brucella spp. in Livestock Slaughtered from Abattoirs in Eastern Cape, South Africa

Snakes and Souks: Zoonotic pathogens associated to reptiles in the Marrakech markets, Morocco

Polyphasic Characterization of Brucella spp. in Livestock Slaughtered from Abattoirs in Eastern Cape, South Africa

Contact Info

Product

Resources

About