Multilocus nuclear DNA markers and genetic parameters in an Indian Anopheles minimus population

Dixit, Jyotsana; Srivastava, Hemlata; Singh, O. P.; Saksena, D. N.; Das, Ashim

doi:10.1016/j.meegid.2011.01.001

Cited by 8 publications

(23 citation statements)

References 51 publications

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“…The details on the genetic locations, primer sequences for PCR and sequencing of seven DNA fragments have been reported earlier (Dixit et al . ).…”

Section: Methodsmentioning

confidence: 97%

“…The eighth fragment [M3 (Dixit et al . )] did not produce proper PCR amplification/sequencing in all the samples; thus, this fragment could not be considered in this study. The seven homologous sequences of Anopheles fluviatilis sequenced earlier (Dixit et al .…”

Section: Methodsmentioning

confidence: 99%

“…The seven homologous sequences of Anopheles fluviatilis sequenced earlier (Dixit et al . ; GenBank accession nos given in Table S1, Supporting information) have also been utilized for different statistical analyses (see below). The details on the genetic locations, primer sequences for PCR and sequencing of seven DNA fragments have been reported earlier (Dixit et al .…”

Section: Methodsmentioning

confidence: 99%

“…; Dixit et al . ) and extension at 72 °C for 1 min, followed by final extension at 72 °C for 5 min. PCR‐amplified fragments that show a clear single band without any primer dimer were subjected to purification using EXO‐SAP (Fermentas, Life Sciences).…”

Section: Methodsmentioning

confidence: 99%

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Multilocus nuclear DNA markers reveal population structure and demography of Anopheles minimus

et al. 2014

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Utilization of multiple putatively neutral DNA markers for inferring evolutionary history of species population is considered to be the most robust approach. Molecular population genetic studies have been conducted in many species of Anopheles genus, but studies based on single nucleotide polymorphism (SNP) data are still very scarce. Anopheles minimus is one of the principal malaria vectors of Southeast (SE) Asia including the Northeastern (NE) India. Although population genetic studies with mitochondrial genetic variation data have been utilized to infer phylogeography of the SE Asian populations of this species, limited information on the population structure and demography of Indian An. minimus is available. We herewith have developed multilocus nuclear genetic approach with SNP markers located in X chromosome of An. minimus in eight Indian and two SE Asian population samples (121 individual mosquitoes in total) to infer population history and test several hypotheses on the phylogeography of this species. While the Thai population sample of An. minimus presented the highest nucleotide diversity, majority of the Indian samples were also fairly diverse. In general, An. minimus populations were moderately substructured in the distribution range covering SE Asia and NE India, largely falling under three distinct genetic clusters. Moreover, demographic expansion events could be detected in the majority of the presently studied populations of An. minimus. Additional DNA sequencing of the mitochondrial COII region in a subset of the samples (40 individual mosquitoes) corroborated the existing hypothesis of Indian An. minimus falling under the earlier reported mitochondrial lineage B.

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“…The details on the genetic locations, primer sequences for PCR and sequencing of seven DNA fragments have been reported earlier (Dixit et al . ).…”

Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Multilocus nuclear DNA markers reveal population structure and demography of Anopheles minimus

et al. 2014

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“…have revolutionized the field of population genetics through the choice of appropriate markers to answer specific evolutionary questions (Batzer and Deininger 2002;Bamshad et al 2004;Yotova et al 2007;Cox et al 2008;Shi et al 2010;Underhill et al 2010). Since it is now known that choice of genetic markers highly influences evolutionary assumptions (Baines et al 2004;Das et al 2004), choosing multiple genetic markers without the influence of natural selection (otherwise termed as 'putatively neutral') is the best choice to infer population structure and demography of species populations Gupta et al 2010Gupta et al , 2012Dixit et al 2011). In humans, due to the availability of whole genome sequence information and the parallel development in statistical analyses of DNA sequence data in recent years, Single Nucleotide Polymorphisms (SNPs) have dominated as 'markers of choice' for utilization in different population genetic model testing and inferring the evolutionary history of genes, genomes and human populations (Stajich and Hahn 2004;Labate et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Development of multilocus putatively neutral DNA markers in the X-chromosome for population genetic studies in humans

Khan

Chittoria

Pande

et al. 2012

Annals of Human Biology

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Molecular population genetics of the NADPH cytochrome P450 reductase (CPR) gene in Anopheles minimus

et al. 2014

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Development of insecticide resistance (IR) in mosquito vectors is a primary huddle to malaria control program. Since IR has genetic basis, and genes constantly evolve with response to environment for adaptation to organisms, it is important to know evolutionary pattern of genes conferring IR in malaria vectors. The mosquito Anopheles minimus is a major malaria vector of the Southeast (SE) Asia and India and is susceptible to all insecticides, and thus of interest to know if natural selection has shaped variations in the gene conferring IR. If not, the DNA fragment of such a gene could be used to infer population structure and demography of this species of malaria vector. We have therefore sequenced a ~569 bp DNA segment of the NADPH cytochrome P450 reductase (CPR) gene (widely known to confer IR) in 123 individuals of An. minimus collected in 10 different locations (eight Indian, one Thai and one Vietnamese). Two Indian population samples were completely mono-morphic in the CPR gene. In general, low genetic diversity was found with no evidence of natural selection in this gene. The data were therefore analyzed to infer population structure and demography of this species. The 10 populations could be genetically differentiated into four different groups; the samples from Thailand and Vietnam contained high nucleotide diversity. All the 10 populations conform to demographic equilibrium model with signature of past population expansion in four populations. The results in general indicate that the An. minimus mosquitoes sampled in the two SE Asian localities contain several genetic characteristics of being parts of the ancestral population.

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Multilocus nuclear DNA markers and genetic parameters in an Indian Anopheles minimus population

Cited by 8 publications

References 51 publications

Multilocus nuclear DNA markers reveal population structure and demography of Anopheles minimus

Multilocus nuclear DNA markers reveal population structure and demography of Anopheles minimus

Development of multilocus putatively neutral DNA markers in the X-chromosome for population genetic studies in humans

Molecular population genetics of the NADPH cytochrome P450 reductase (CPR) gene in Anopheles minimus

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