Multilocus Sequence Analysis for Assessment of Phylogenetic Diversity and Biogeography in Thalassospira Bacteria from Diverse Marine Environments

Abstract

Thalassospira bacteria are widespread and have been isolated from various marine environments. Less is known about their genetic diversity and biogeography, as well as their role in marine environments, many of them cannot be discriminated merely using the 16S rRNA gene. To address these issues, in this report, the phylogenetic analysis of 58 strains from seawater and deep sea sediments were carried out using the multilocus sequence analysis (MLSA) based on acsA, aroE, gyrB, mutL, rpoD and trpB genes, and the DNA-DNA hybridization (DDH) and average nucleotide identity (ANI) based on genome sequences. The MLSA analysis demonstrated that the 58 strains were clearly separated into 15 lineages, corresponding to seven validly described species and eight potential novel species. The DDH and ANI values further confirmed the validity of the MLSA analysis and eight potential novel species. The MLSA interspecies gap of the genus Thalassospira was determined to be 96.16–97.12% sequence identity on the basis of the combined analyses of the DDH and MLSA, while the ANIm interspecies gap was 95.76–97.20% based on the in silico DDH analysis. Meanwhile, phylogenetic analyses showed that the Thalassospira bacteria exhibited distribution pattern to a certain degree according to geographic regions. Moreover, they clustered together according to the habitats depth. For short, the phylogenetic analyses and biogeography of the Thalassospira bacteria were systematically investigated for the first time. These results will be helpful to explore further their ecological role and adaptive evolution in marine environments.

Genomic diversity within the haloalkaliphilic genus Thioalkalivibrio

“…For this reason, this analysis can only distinguish between different Thioalkalivibrio species at a low resolution, which was previously observed for other bacteria [72,39], such as Hyphomonas [73], Thalassospira [74], Acinetobacter [75], Nocardia [76] and Bifidobacterium [77]. Therefore, species affiliation cannot be based on 16S rRNA gene sequence analysis alone due to the fact that different taxa might have different diversification rates of their 16S rRNA gene sequences [78].…”

Simultaneous ammonia and nitrate removal in an airlift reactor using poly(butylene succinate) as carbon source and biofilm carrier

“…Thalassospira has been found to contain bio-degradation ability in marine sediments and perform denitrifying processes in wastewater treatment, which might account for the biodegradation and nitrate removal (Lai et al, 2014). In addition, the relative abundance of Bowmanella increased during the whole experiment, which was detected from a pyrene-degrading consortium in previous study, and was discovered to harbor denitrification ability (Lai et al, 2014). On the basis of the above discussion, our reactor was dominated by denitrifiers some of which maintained biodegradability or oxygen-endure ability.…”

Optimization of Lipase production from a novel strainThalassospira permensisM35-15 using Response Surface Methodology

“…[3][4][5] Thalassospira are a genus created in 2002 and currently have 8 validly named species belonging to Rhodospirillaceae. [6][7][8] However, there was no report on Thalassospira permensis producing lipases to our knowledge.…”