Multilocus sequence analysis reveals extensive genetic variety within Tenacibaculum spp. associated with ulcers in sea-farmed fish in Norway

Olsen, Anne Berit; Gulla, Snorre; Steinum, Terje; Colquhoun, Duncan J.; Nilsen, Hanne; Duchaud, Éric

doi:10.1016/j.vetmic.2017.04.028

Cited by 37 publications

(80 citation statements)

References 21 publications

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“…This variation in DNA for all the 12 isolates indicates a co-infection with several genotypes of T. dicentrarchi. The finding that the DNA sequences of all the studied isolates have minor variations despite the fact that the isolates were obtained from the same site and the same outbreak of disease, and some also from the same tank and the same fish, partly supports previous reports that Tenacibaculum infections often involve multiple strains that lack strain clonality even from the same disease cases, and change genotype rapidly (Olsen et al, 2017). This type of co-infection is common in bacterial diseases in fish and has also been reported for many other Flavobacterium sp.…”

Section: Cultivation From the Fishsupporting

confidence: 84%

“…(Figure 7). The Tenacibaculum isolates obtained from the current study also clustered with a Norwegian isolate from cod (TNO012), which was clustered with T. dicentrarchi (Olsen et al, 2017). The 12 isolates from the current study seem to be a clonal complex or similar genotype since all were tightly clustered except isolate 05, which was clustered a bit separately in most gene trees though all were located within the T. dicentrarchi cluster.…”

Section: S Rrna Analysismentioning

confidence: 59%

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An outbreak of acute disease and mortality in Atlantic salmon (Salmo salar) post‐smolts in Norway caused by Tenacibaculum dicentrarchi

Klakegg

Abayneh

Fauske

et al. 2019

Journal of Fish Diseases

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An outbreak of disease characterized by skin ulcers, fin rot and mortality was observed a few days after the transfer of Atlantic salmon (Salmo salar) from a freshwater smolt production facility to a land‐based seawater post‐smolt site. Dead and moribund fish had severe skin and muscle ulcers, often 2–6 cm wide, particularly caudal to the pectoral fins. Microscopic examination of smears from ulcers and head kidney identified long, slender Gram‐negative rods. Histopathological analysis revealed abundance of long, slender Tenacibaculum‐like bacteria in ulcers and affected fins. Genetic characterization using multilocus sequence analysis (MLSA) of seven housekeeping genes, including atpA, dnaK, glyA, gyrB, infB, rlmN and tgt, revealed that the isolates obtained during the outbreak were all clustered with the Tenacibaculum dicentrarchi‐type strain (USC39/09T) from Spain. Two bath challenge experiments with Atlantic salmon and an isolate of T. dicentrarchi from the outbreak were performed. No disease or mortality was observed in the first trial. In the second trial with a higher challenge dose of T. dicentrarchi and longer challenge time, we got 100% mortality within 48 hr. This is the first reported outbreak of disease caused by T. dicentrarchi in Norwegian farmed Atlantic salmon.

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Section: Cultivation From the Fishsupporting

confidence: 84%

Section: S Rrna Analysismentioning

confidence: 59%

An outbreak of acute disease and mortality in Atlantic salmon (Salmo salar) post‐smolts in Norway caused by Tenacibaculum dicentrarchi

Klakegg

Abayneh

Fauske

et al. 2019

Journal of Fish Diseases

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show abstract

“…In accordance with Olsen et al. (2017) and Bridel et al. (2018), phylogenetic analyses with single and concatenated genes included all published sequences for each housekeeping gene of T. dicentrarchi strains (3509 T , TNO021, TdChD05), “ T. finnmarkense ” (HFJ T , TNO006, AY7486TD) and Tenacibaculum sp.…”

Section: Methodsmentioning

confidence: 99%

New salmonid hosts for Tenacibaculum species: Expansion of tenacibaculosis in Chilean aquaculture

Avendaño‐Herrera

Collarte

Saldarriaga‐Córdoba

et al. 2020

Journal of Fish Diseases

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The success and sustainability of Chilean aquaculture largely depends on the control of endemic and emerging pathogens, including several species of the genus Tenacibaculum. Tenacibaculum dicentrarchi and “Tenacibaculum finnmarkense” have been detected and confirmed in Chilean Atlantic salmon (Salmo salar). However, no outbreaks of tenacibaculosis in rainbow trout (Oncorhynchus mykiss) or coho salmon (Oncorhynchus kisutch) have been reported, either in Chile or globally. The aims of this study were to determine whether the mortalities recorded for rainbow trout and coho salmon from five marine fish farms located in the Los Lagos, Aysén, and Magallanes Regions could be caused by Tenacibaculum spp. The diseased fish exhibited cutaneous haemorrhages, tail and peduncle rots, and damage on the mouth and tongue. Microbiological analysis of infected external tissues yielded 13 bacterial isolates. The isolates were identified as members of the genus Tenacibaculum through biochemical analysis (e.g. Gram‐stain negative, straight rods, filamentous cells and motile by gliding), but differences existed in biochemical results, making species‐level identification through biomolecular tools essential. The 16S rRNA analysis found that the majority of isolates were more closely related to “T. finnmarkense” than T. dicentrarchi, while the phylogenetic trees resulting from multilocus sequence data recovered the four main clades (clades I to IV) identified by Olsen et al. (2017, Veterinary Microbiology, 205, 39). This is the first documented occurrence of clinical tenacibaculosis in farmed rainbow trout and coho salmon globally, and it extends the known host distribution of this pathogen in Chile. Moreover, we confirm the presence of Tenacibaculum species in the Chilean Patagonia. These findings highlight the importance of establishing preventative measures to minimize the spread of this disease within the Chilean marine aquaculture industry, as well as the need for monitoring initiatives worldwide in these farmed fish species.

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“…Piñeiro-Vidal, Gijón, Zarza, & Santos, 2012), but has also been identified in Atlantic salmon (Salmo salar) in Chile and Norway (Avendaño-Herrera et al, 2016;Olsen et al, 2017), as well as in red conger eel (Genypterus chilensis) in Chile (Irgang et al, 2017).…”

Section: Tenacibaculosis Tenacibaculum Dicentrarchimentioning

confidence: 99%

“…Tenacibaculosis is a disease commonly reported in farmed fish in association with Tenacibaculum maritimum and related bacterial species, such as Tenacibaculum dicentrarchi . This latter bacterium was first isolated in Spain from European sea bass ( Dicentrarchus labrax L; Piñeiro‐Vidal, Gijón, Zarza, & Santos, ), but has also been identified in Atlantic salmon ( Salmo salar ) in Chile and Norway (Avendaño‐Herrera et al, ; Olsen et al, ), as well as in red conger eel ( Genypterus chilensis ) in Chile (Irgang et al, ).…”

mentioning

confidence: 99%

A high‐throughput analysis of biofilm formation by the fish pathogen Tenacibaculum dicentrarchi

Levipan

Irgang

Tapia‐Cammas

et al. 2019

Journal of Fish Diseases

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Multilocus sequence analysis reveals extensive genetic variety within Tenacibaculum spp. associated with ulcers in sea-farmed fish in Norway

Cited by 37 publications

References 21 publications

An outbreak of acute disease and mortality in Atlantic salmon (Salmo salar) post‐smolts in Norway caused by Tenacibaculum dicentrarchi

An outbreak of acute disease and mortality in Atlantic salmon (Salmo salar) post‐smolts in Norway caused by Tenacibaculum dicentrarchi

New salmonid hosts for Tenacibaculum species: Expansion of tenacibaculosis in Chilean aquaculture

A high‐throughput analysis of biofilm formation by the fish pathogen Tenacibaculum dicentrarchi

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