Multilocus Sequence Types of Campylobacter jejuni Isolates from Different Sources in Eastern China

Zhang, Gong; Zhang, Xiaoyan; Hu, Yuanqing; Jiao, Xinan; Huang, Jinlin

doi:10.1007/s00284-015-0853-3

Cited by 14 publications

(9 citation statements)

References 32 publications

(36 reference statements)

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“…In North China, the most frequently isolated clonal complexes were CC-21, CC-353, CC-354 and CC-443 [ 31 , 35 ], while the dominant clonal complexes of C. coli were CC-828 and CC-1150 [ 36 ]. In East China, the most common ST type of the Campylobacter strains isolated from human and food was ST-353, while the dominant ST type from chicken and food was ST-354 [ 10 ]. In Guangdong, a province in southern China, the dominant clonal complex was CC-828 [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…From 2008 to 2014, Wang et al reported that the positive rates of C. jejuni and C. coli were 18.1 and 19.0% respectively in five provinces of China [ 9 ]. Zhang et al analyzed the genetic diversity of the C. jejuni isolates in Eastern China by multilocus sequence typing (MLST) and identified 94 sequence types (STs) belonging to 18 clonal complexes (CCs) [ 10 ]. However, data on the prevalence and genetic diversity of Campylobacter is still limited in China, especially central China, which is an important transportation junction.…”

Section: Introductionmentioning

confidence: 99%

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Genotypic diversity, antimicrobial resistance and biofilm-forming abilities of Campylobacter isolated from chicken in Central China

et al. 2017

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Background Campylobacter is considered to be the leading cause of human bacterial gastroenteritis, of which poultry is the main reservoir. Campylobacter contaminated chicken products are a major cause of human Campylobacter infection. In this study, the prevalence of Campylobacter in chicken in central China was investigated, and the genotypic diversity, antimicrobial resistance and biofilm of these isolates were characterized.ResultsA total of 206 Campylobacter isolates, including 166 C. jejuni and 40 C. coli, were isolated from chicken farms and live poultry markets in central China. Multilocus sequence typing and phylogenetic analysis showed that the Campylobacter isolates had diverse genetic backgrounds, which covered most of the dominant clone complexes (CCs) reported throughout China. The most prevalent CCs were CC-464, CC-1150, CC-353, and CC-828. All the isolates showed resistance to norfloxacin, ciprofloxacin and Cefazolin, and a prevalent resistance to fluoroquinolones, β-lactams and tetracyclines was also observed. Among all the isolates, 133 strains showed the ability to form biofilm, thereinto, the isolates in two genetic branches, mainly including CC-21, CC-48, CC-677 and CC-45, showed a significantly lower ability to form biofilm than other genetic branches (p < 0.05). However, in general, the ability to form biofilm varied among different genetic branches, suggesting a complex genetic background to biofilm formation, but not only the genetic lineages. Compared with the strains unable to form biofilm, biofilm-producing strains possessed a significantly higher resistance to ampicillin, neomycin, sulfamethoxazole, amikacin, clindamycin and erythromycin (p < 0.05).ConclusionsTo the best of our knowledge, this is the first report on the relationship of the genotypic diversity, antimicrobial resistance and biofilm-forming abilities of Campylobacter isolated from chicken in Central China, which showed the potential importance of biofilm in antimicrobial resistance. This study will help us better understand the epidemiology and antimicrobial resistance of Campylobacter.Electronic supplementary materialThe online version of this article (10.1186/s13099-017-0209-6) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genotypic diversity, antimicrobial resistance and biofilm-forming abilities of Campylobacter isolated from chicken in Central China

et al. 2017

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“…In the UK, CCs ST-21 and ST-45 are commonly detected in veterinary (i.e., cow, pet, sheep, and poultry) and human sources ( Manning et al, 2003 ). CCs 21, 45, 353, and 354 are also highly prevalent in sources from humans and foods in Eastern China ( Zhang et al, 2015 ). According to the results of an MLST analysis of 122 C. jejuni isolates from Danish patients with symptoms of gaostroenteritis, reactive arithritis, and Guillain–Barré syndrome, CCs ST-21, -45, and -22 are highly prevalent, accounting for 64% of all C. jejuni isolates from humans.…”

Section: Discussionmentioning

confidence: 99%

High Prevalence of Hyper-Aerotolerant Campylobacter jejuni in Retail Poultry with Potential Implication in Human Infection

McMullen

Jeon

2015

Front. Microbiol.

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Campylobacter jejuni is a leading cause of foodborne illnesses around the world. Since C. jejuni is microaerophilic and sensitive to oxygen, aerotolerance is important in the transmission of C. jejuni to humans via foods under aerobic conditions. In this study, 70 C. jejuni strains were isolated from retail raw chicken meats and were subject to multilocus sequence typing (MLST) analysis. In the aerotolerance testing by aerobic shaking at 200 rpm, 50 (71.4%) isolates survived after 12 h (i.e., aerotolerant), whereas 20 (28.6%) isolates did not (i.e., aerosensitive). Interestingly, further aerobic cultivation showed that 25 (35.7%) isolates still survived even after 24 h of vigorous aerobic shaking (i.e., hyper-aerotolerant). Compared to aerosensitive strains, the hyper-aerotolerant strains exhibited increased resistance to oxidative stress, both peroxide and superoxide. A mutation of ahpC in hyper-aerotolerant strains significantly impaired aerotolerance, indicating oxidative stress defense plays an important role in hyper-aerotolerance. The aerotolerant and hyper-aerotolerant strains were primarily classified into MLST clonal complexes (CCs)-21 and -45, which are known to be the major CCs implicated in human gastroenteritis. Compared to the aerosensitive strains, CC-21 was more dominant than CC-45 in aerotolerant and hyper-aerotolerant strains. The findings in this study revealed that hyper-aerotolerant C. jejuni is highly prevalent in raw chicken meats. The enhanced aerotolerance in C. jejuni would impact human infection by increasing possibilities of the foodborne transmission of C. jejuni under aerobic conditions.

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“…coli [11]. Zhang et al analyzed the genetic diversity of the C. jejuni isolates in Eastern China by multilocus sequence typing (MLST) and defined 94 sequence types (STs) belonging to 18 clonal complexes (CCs) [12]. To my best knowledge, few data were reported on the prevalence and contamination level of Campylobacter in chicken products in Central China, so the risk assessments related to food safety are also hampered by the lack of basic data.…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology, virulence determinants and antimicrobial resistance of Campylobacter spreading in retail chicken meat in Central China

et al. 2016

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Background Campylobacter species are the major food-borne pathogens which could cause bacterial gastroenteritis in humans. Contaminated chicken products have been recognized as the primary vehicles of Campylobacter transmission to human beings. In this study, the prevalence of Campylobacter in retail chicken meat in Central China was investigated, and the isolates were further characterized using molecular approaches and tested for antibiotic resistance.ResultsA total of 302 chicken samples purchased from April 2014 to April 2015 were tested. The level of Campylobacter contamination was enumerated by most probable number-PCR (MPN-PCR). The Campylobacter positive rate was 17.2% (52/302), with bacterial count varying from 3.6 to 360 MPN/g in positive samples. A total of 52 Campylobacter strains, including 40 Campylobacter jejuni and 12 Campylobacter coli, were isolated from the positive samples. To examine the genetic diversity of the isolates, multilocus sequence typing (MLST) technology was applied, which identified 23 sequence types (STs) belonging to seven clonal complexes (CCs) and unassigned. Among them, the dominant CCs of C. jejuni included CC-353 and CC-464, and the dominant CCs of C. coli were CC-828 and CC-1150. Antibiotic resistance analysis showed that all of the isolates were resistant to norfloxacin and ciprofloxacin. 23 virulence-associated genes were tested in the isolates, which showed that the number of virulence-associated genes detected in the C. jejuni isolates ranged from 16 to 21, while in most of the C. coli isolates ranged from 12 to 16. Virulence-associated genes, flaA, flgB, flgE2, fliM, fliY and cadF were detected in all isolates. VirB11, however, was not detected in any of the isolates.ConclusionsTo the best of our knowledge, this is the first report on the contamination level and molecular biological features of Campylobacter strains in retail chicken meat in Central China, which showed high genetic diversity and remarkable antibiotic resistance. This study provided scientific data for the risk assessment and evaluation of Campylobacter contamination in retail chicken products.Electronic supplementary materialThe online version of this article (doi:10.1186/s13099-016-0132-2) contains supplementary material, which is available to authorized users.

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Multilocus Sequence Types of Campylobacter jejuni Isolates from Different Sources in Eastern China

Cited by 14 publications

References 32 publications

Genotypic diversity, antimicrobial resistance and biofilm-forming abilities of Campylobacter isolated from chicken in Central China

Genotypic diversity, antimicrobial resistance and biofilm-forming abilities of Campylobacter isolated from chicken in Central China

High Prevalence of Hyper-Aerotolerant Campylobacter jejuni in Retail Poultry with Potential Implication in Human Infection

Molecular epidemiology, virulence determinants and antimicrobial resistance of Campylobacter spreading in retail chicken meat in Central China

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