Multilocus Sequence Typing for Differentiation of Strains of
            <i>Candida tropicalis</i>

Tavanti, Arianna; Davidson, Amanda Denise; Johnson, Elizabeth; Maiden, Martin C. J.; Shaw, Duncan; Gow, Neil A. R.; Odds, Frank C.

doi:10.1128/jcm.43.11.5593-5600.2005

Cited by 131 publications

(155 citation statements)

References 23 publications

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“…Whether any of these isolates belong to the R 5FC clone would be of interest. Of note, a recent study of 104 C. tropicalis clinical isolates recovered from various countries (9) showed that none of the 5 R 5FC isolates collected in the United Kingdom has the MLST profi le of the R 5FC clone.…”

Section: Discussionmentioning

confidence: 99%

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Clonal Population of Flucytosine-ResistantCandida tropicalisfrom Blood Cultures, Paris, France

Desnos‐Ollivier

Bretagne²,

Bernède³

et al. 2008

Emerg. Infect. Dis.

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Candida tropicalis is a diploid ascomycetes yeast responsible for 4%-24% of candidemia. Resistance to fl ucytosine is rarely described for this species but was observed for 45 (35%) of 130 C. tropicalis isolates recovered from blood cultures in the Paris area in a 4-year survey. The aims of this study were to test the hypothesis that the fl ucytosine-resistant isolates could represent a subgroup and to determine the relationship between epidemiologic and genomic data. Epidemiologic data and gene sequences were analyzed, and molecular typing was performed. Our results suggest that a clone of fl ucytosine-resistant isolates, associated with malignancies and a lower mortality than that for other C. tropicalis isolates, is widespread in the Paris area. We propose the analysis of 2 polymorphic microsatellite markers coupled with URA3 sequencing to track the clone. C andida tropicalis is a diploid ascomycetes yeast commonly found on the skin and in digestive tracts of healthy human hosts worldwide (1). Infections caused by C. tropicalis are reported in 4%-24% of patients with candidemia, depending on the country of study, underlying risk factors, and period of study (2). Primary resistance to fl ucytosine (5FC) occurs in <5% of all Candida species except for C. krusei, in which it is detected in up to 28% of isolates (3). It was thus unexpected to observe 35% resistance to 5FC among C. tropicalis isolates recovered from blood cultures in the active surveillance program on yeast-related fungemia implemented by the French National Reference Center for Mycoses and Antifungals (NRCMA) in the Paris area. The YEASTS program is designed to analyze the epidemiologic trends of yeast fungemia by collecting isolates and epidemiologic and clinical data. The second objective is to study the clinical isolates in terms of species, antifungal susceptibility profi les, and genetic diversity to look for associations between subtypes of isolates and epidemiologic/clinical parameters. To test the hypothesis that the 5FC resistant ( R 5FC) isolates could represent a different species or a subgroup, the R 5FC and susceptible ( S 5FC)

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Section: Discussionmentioning

confidence: 99%

“…Three of the 6 MLST loci recently described were analyzed as reported in Tavanti et al (9). These loci were selected because, according to these authors, they were associated with more polymorphism (XYR1 and SAPT4) and with antifungal resistance (MDR1).…”

Section: Multilocus Sequence Typing Analysismentioning

confidence: 99%

Clonal Population of Flucytosine-ResistantCandida tropicalisfrom Blood Cultures, Paris, France

Desnos‐Ollivier

Bretagne²,

Bernède³

et al. 2008

Emerg. Infect. Dis.

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“…There are reports about its application for several Candida species (86,116). Recently, two new techniques arouse: amplified fragment length polymorphism (AFLP) (3) and multilocus sequence typing (MLST) (12,32,115).…”

Section: Molecular Biologymentioning

confidence: 99%

Oral Candida spp. colonization in human immunodeficiency virus-infected individuals

Moris¹,

Melhem²,

Martins³

et al. 2008

J. Venom. Anim. Toxins incl. Trop. Dis

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“…glabrata (Dodgson et al, 2003), C. tropicalis (Tavanti et al, 2005), C. krusei , Cryptococcus neoformans var.…”

Section: Multi-locus Sequence Typing (Mlst) 분석mentioning

confidence: 99%

Genetic Variations ofAspergillus fumigatusClinical Isolates from Korea

Kim

Park

et al. 2017

BSL

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Fungal infections by human pathogenic fungi are increasing globally in elderly, children and immune suppressed or deficient patients. Aspergillus fumigatus is one of the well-known pathogenic fungi and causes aspergilloses in human world widely. However, current identification and classification methods based on its phenotypic characteristics still have limitations. Therefore, currently, molecular biological tools using their DNA sequences are used for genotype identification and classification. In the present study, in order to analyze genetic variations of A. fumigatus clinical isolates, a total of six housekeeping genes were amplified by PCR using specific primer pairs and multi-locus sequence typing (MLST) assay. Results from phylogenetic tree analysis showed that most A. fumigatus strains (88.9%) from respiratory specimens were classified into cluster A and B, and approximately half of A. fumigatus strains (46%) from non-respiratory specimens were classified into cluster C and D. Although the sample size was limited, genetic characteristics of A. fumigatus clinical isolates according to their origins were very similar and well-correlated with other clinical data.

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Multilocus Sequence Typing for Differentiation of Strains of Candida tropicalis

Cited by 131 publications

References 23 publications

Clonal Population of Flucytosine-ResistantCandida tropicalisfrom Blood Cultures, Paris, France

Clonal Population of Flucytosine-ResistantCandida tropicalisfrom Blood Cultures, Paris, France

Oral Candida spp. colonization in human immunodeficiency virus-infected individuals

Genetic Variations ofAspergillus fumigatusClinical Isolates from Korea

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