Multilocus sequence typing of a global collection of Pasteurella multocida isolates from cattle and other host species demonstrates niche association

Abstract

BackgroundPasteurella multocida causes disease in many host species throughout the world. In bovids, it contributes to bovine respiratory disease (BRD) and causes haemorrhagic septicaemia (HS). Previous studies have suggested that BRD-associated P. multocida isolates are of limited diversity. A multilocus sequence typing (MLST) scheme for P. multocida was used to determine whether the low levels of diversity reported are due to the limited discriminatory power of the typing method used, restricted sample selection or true niche association. Bovine respiratory isolates of P. multocida (n = 133) from the UK, the USA and France, collected between 1984 and 2008 from both healthy and clinically affected animals, were typed using MLST. Isolates of P. multocida from cases of HS, isolates from other host species and data from the MLST database were used as comparison.ResultsBovine respiratory isolates were found to be clonal (ISA 0.45) with 105/128 belonging to clonal complex 13 (CC13). HS isolates were not related to bovine respiratory isolates. Of the host species studied, the majority had their own unique sequence types (STs), with few STs being shared across host species, although there was some cross over between porcine and bovine respiratory isolates. Avian, ovine and porcine isolates showed greater levels of diversity compared to cattle respiratory isolates, despite more limited geographic origins.ConclusionsThe homogeneity of STs of bovine respiratory P. multocida observed, and the differences between these and P. multocida subpopulations from bovine non-respiratory isolates and non-bovine hosts may indicate niche association.

Molecular characterization of Pasteurella multocida isolates from swine lungs by Randomly Amplified Polymorphic DNA

“…Most of the isolates from this study belong to ST74 and ST13, which was similar to swine in Norway (PORS et al, 2011), except that ST50 was absent in our study. These isolates are probably species-specific (HOTCHKISS et al, 2011) with the exception of ST13, which has also been identified in cattle.…”

Studies on the presence and persistence ofPasteurella multocidaserovars and genotypes in fowl cholera outbreaks

“…The power of MLST is that isolates can be typed anywhere in the world and then directly compared with a publicly accessible database, allowing comparisons across laboratories and time (Enright & Spratt, 1998). It has been shown that there are strong host associations between some clones of P. multocida and certain hosts with few STs being shared across host species (Hotchkiss et al, 2011). The use of MLST has shown that some of the isolates recovered in the study are apparently strongly linked with avian hosts (ST 8 and ST 20) while other isolates, for example, are in ST 9, an ST that has been recovered from eight different host types, ranging from humans to domestic livestock to a zoo animal.…”

Multilocus sequence analysis of Pasteurella multocida demonstrates a type species under development

“…When all STs in the RIRDC database were compared in a concatenated phylogenetic and coalescence analysis, it was impossible to further associate the groups to 16S rRNA and rpoB phylogenies since such information was not available in the major studies of the STs (Hotchkiss et al, 2011;Pors et al, 2011). For all STs compared both by concatenated and coalescence analysis, the A/B group included 13 STs.…”

“…Unfortunately, the study only included isolates from fowl cholera in Australia and consequently gave no insight as to the possible existence of host-and/or disease-related clonal lineages in addition to lineages that might have an increased propensity to cause disease. Recent MLST-based investigations have mainly focused on the association between hosts and lesion types, and included P. multocida isolated from calves and pigs (Hotchkiss et al, 2011;McFadden et al, 2011;Pors et al, 2011). In addition, case reports have been provided from apes and kangaroos (Köndgen et al, 2011;Bertelsen et al, 2012).…”