Multilocus Sequence Typing Supports the Hypothesis that Cow- and Human-Associated<i>Salmonella</i>Isolates Represent Distinct and Overlapping Populations

Alcaine, Samuel D.; Soyer, Yeşim; Warnick, Lorin D.; Su, Wan-Lin; Sukhnanand, Sharinne; Richards, J. D.; Fortes, Esther D.; McDonough, Patrick L.; Root, Timothy P.; Dumas, Nellie B.; Gröhn, Yrjö T.; Wiedmann, Martin

doi:10.1128/aem.01174-06

Cited by 60 publications

(82 citation statements)

References 45 publications

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“…On the other hand, Salmo- nella characterization methods that allow for phylogeny reconstruction (e.g., MLST) might provide some advantages over traditional or molecular serotyping approaches, which do not provide phylogenetic information. For example, MLST can differentiate isolates into distinct phylogenetic groups even if they represent the same serovar, and this has been demonstrated for a number of polyphyletic serovars (51).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Typing Methods with a New Procedure Based on Sequence Characterization for Salmonella Serovar Prediction

et al. 2013

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bAs the development of molecular serotyping approaches is critical for Salmonella spp., which include >2,600 serovars, we performed an initial evaluation of the ability to identify Salmonella serovars using (i) different molecular subtyping methods and (ii) a newly implemented combined PCR-and sequencing-based approach that directly targets O-and H-antigen-encoding genes. Initial testing was performed using 46 isolates that represent the top 40 Salmonella serovars isolated from human and nonhuman sources, as reported by the U.S. Centers for Disease Control and Prevention and the World Health Organization. Multilocus sequence typing (MLST) was able to accurately predict the serovars for 42/46 isolates and showed the best ability to predict serovars among the subtyping methods tested. Pulsed-field gel electrophoresis (PFGE), ribotyping, and repetitive extragenic palindromic sequence-based PCR (rep-PCR) were able to accurately predict the serovars for 35/46, 34/46, and 30/46 isolates, respectively. Among the methods, S. enterica subsp. enterica serovars 4,5,12:i:؊, Typhimurium, and Typhimurium var. 5؊ were frequently not classified correctly, which is consistent with their close phylogenetic relationship. To develop a PCR-and sequence-based serotyping approach, we integrated available data sources to implement a combination PCR-based O-antigen screening and sequencing of internal fliC and fljB fragments. This approach correctly identified the serovars for 42/46 isolates in the initial set representing the most common Salmonella serovars, as well as for 54/63 isolates representing less common Salmonella serovars. Our study not only indicates that different molecular approaches show the potential to allow for rapid serovar classification of Salmonella isolates, but it also provides data that can help with the selection of molecular serotyping methods to be used by different laboratories.

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Section: Discussionmentioning

confidence: 99%

Comparison of Typing Methods with a New Procedure Based on Sequence Characterization for Salmonella Serovar Prediction

et al. 2013

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“…While traditional MLST schemes target seven housekeeping genes (24), we initially used a previously reported MLST scheme targeting three genes (i.e., manB, mdh, and fimA) (2,33) to characterize all isolates used in this study. In addition to these three genes, we also sequenced an 826-nucleotide fragment of a fourth gene (aroC) in all isolates to determine whether the use of additional genes would increase discriminatory power.…”

Section: Salmonella Isolates a Total Of 190 Salmonella Isolates Initmentioning

confidence: 99%

“…Salmonella serotype 4,5,12:i:Ϫ has been isolated, particularly over the last decade, from a number of different foods and animals (1,6,13,29,38). While a number of separate studies, using molecular subtyping and characterization tools (e.g., genomic microarrays and PCR assays to test for gene presence/absence), have shown that Salmonella serotype 4,5,12:i:Ϫ isolates from Spain (15,18) and the United States (1,2,38) are genetically closely related to Salmonella serotype Typhimurium, we are not aware of any comparative studies of Salmonella serotype 4,5,12:i:Ϫ isolates from Europe and the United States that have been published to date. In order to provide a better understanding of the transmission, ecology, and evolution of Salmonella serotype 4,5,12:i:Ϫ, we have assembled a collection of 190 Salmonella serotype 4,5,12:i:Ϫ and Typhimurium isolates from various sources and from two countries, the United States and Spain.…”

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confidence: 99%

Salmonella enterica Serotype 4,5,12:i:−, an Emerging Salmonella Serotype That Represents Multiple Distinct Clones

Soyer¹,

Switt²,

et al. 2009

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“…"content-markup(./author-grp [1]/aff|./author-grp [1]/dept-list)> Salmonella is one of the most common food-borne pathogens in the United States, with 40,000 confirmed cases reported annually and subclinical infections estimated at 1.2 million per year (http://www.cdc.gov/salmonella/). 1,16 According to surveillance conducted by the Centers for Disease Control and Prevention (CDC) on food-borne illnesses confirmed in 2006, Salmonella infections accounted for 25.6% of hospitalizations and 30.6% of deaths due to food-associated intoxications; thus, these infections represent a significant public health concern. 6,14,16,20,23 Of the Salmonella isolates from human cases of food poisoning in 2005 identified by the CDC's FoodNet, 6 serovars accounted for 61% of isolates recovered: Typhimurium (19%), Enteritidis (18%), Newport (10%), Heidelberg (6%), Javiana (5%), and Salmonella 4,5,12 (3%), all of which have been isolated from animals.…”

mentioning

confidence: 99%

“…?show "fnote_aff1"$^! "content-markup(./author-grp [1]/aff|./author-grp [1]/dept-list)> Salmonella is one of the most common food-borne pathogens in the United States, with 40,000 confirmed cases reported annually and subclinical infections estimated at 1.2 million per year (http://www.cdc.gov/salmonella/). 1,16 According to surveillance conducted by the Centers for Disease Control and Prevention (CDC) on food-borne illnesses confirmed in 2006, Salmonella infections accounted for 25.6% of hospitalizations and 30.6% of deaths due to food-associated intoxications; thus, these infections represent a significant public health concern.…”

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confidence: 99%

Comparison of Salmonella Serovar Isolation and Antimicrobial Resistance Patterns from Porcine Samples Between 2003 and 2008

2010

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Abstract. Food-borne Salmonella infections can produce symptoms from mild gastroenteritis to severe systemic disease and death, representing an important public health issue in U.S. livestock and livestock products, which have been implicated as frequent sources of Salmonella contamination. Concerns have been raised about the spread of antibiotic resistance in Salmonella strains, particularly those that originate from food animal sources, as a result of prophylactic and therapeutic antimicrobial use in these species. Longitudinal comparisons of Salmonella serovars isolated from porcine tissues submitted to the Iowa State University Veterinary Diagnostic Laboratory in 2003 and 2008 were conducted to evaluate changes in serovar dynamics and antimicrobial resistance. Incidence of recovered group C Salmonella enterica serovar Choleraesuis var. Kunzendorf decreased between 2003 and 2008, while recovery of group B strains Salmonella Typhimurium var. 5-(formerly, Copenhagen), Salmonella Agona, Salmonella Derby, Salmonella Heidelberg, and Salmonella Typhimurium increased. Significant changes in resistance interpretation were seen in Salmonella Derby with regard to spectinomycin and sulfadimethoxine; in Salmonella Heidelberg with regard to florfenicol, spectinomycin, and sulfadimethoxine; and in Salmonella Choleraesuis var. Kunzendorf, Salmonella Typhimurium, Salmonella Typhimurium var. 5-, and Salmonella Agona with regard to spectinomycin. Only 2 of 293 isolates in 2003 and 5 of 395 isolates in 2008 were resistant to enrofloxacin. Utilizing antibiotics approved for use in food animals to evaluate antimicrobial resistance provides more specific information on the selection pressure exerted on Salmonella populations through the use of these drugs.

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Multilocus Sequence Typing Supports the Hypothesis that Cow- and Human-AssociatedSalmonellaIsolates Represent Distinct and Overlapping Populations

Cited by 60 publications

References 45 publications

Comparison of Typing Methods with a New Procedure Based on Sequence Characterization for Salmonella Serovar Prediction

Comparison of Typing Methods with a New Procedure Based on Sequence Characterization for Salmonella Serovar Prediction

Salmonella enterica Serotype 4,5,12:i:−, an Emerging Salmonella Serotype That Represents Multiple Distinct Clones

Comparison of Salmonella Serovar Isolation and Antimicrobial Resistance Patterns from Porcine Samples Between 2003 and 2008

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