Multilocus variation in cercariae, parthenogenetic progeny of different species of the class trematoda

Semyenova, S. K.; Khrisanfova, G. G.; Korsunenko, A. V.; Voronin, M. V.; Beer, Steven V.; Vodyanitskaya, S. V.; Сербина, Е. А.; Yurlova, Natalia I.; Ryskov, A. P.

doi:10.1134/s0012496607030192

Cited by 10 publications

(11 citation statements)

References 7 publications

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“…In contrast, the long proliferation period of germinal cells during the parthenitae development was found in more specialized groups (Sanguinicolidae, Schistosomatidae, Bucephalidae, Diplostomatidae, Strigeidae, Plagiorchiidae) [3]. These findings are well supported by our data obtained earlier [15] in that we found that species which have more germinal cell proliferation cycles tend to exhibit higher levels of clonal variability. Based on RAPD markers we revealed significant genetic heterogeneity (the percentage of polymorphic loci (P) ranged from 17.8% -29.4%) between cercariae within daughter sporocysts of five specialized forms of digenean trematodes (Schistosomatidae, Gorgoderidae, Bucephalidae, Diplostomatidae, Plagiorchiidae).…”

Section: Discussionsupporting

confidence: 91%

“…To detect clonal variability among individual cercariae within sporocysts of T. szidati we selected three random primers (P29, OPA09, OPA10) which previously determined the largest number of polymorphic markers in cercariae RAPD profiles [15]. These primers yielded a total of 105 RAPD bands in the range of 220 -2000 bp.…”

Section: Resultsmentioning

confidence: 99%

“…Since RAPD assay is a method suitable for DNA polymorphism detection in organisms containing even small amounts of sample material we recently used this technique to reveal clonal heterogeneity between individual cercariae within sporocysts and rediae of 10 digenean trematodes from Schistosomatidae, Strigeidae, Gorgoderidae, Bucephalidae, Diplostomatidae, Plagiorchiidae, Halipegidae, Notocotylidae, and Echinostomatidae families [15].…”

Section: Introductionmentioning

confidence: 99%

“…Here, we characterized RAPD fragments revealing clonal variability between individual cercariae within daughter sporocysts of the avian schistosome T. szidati since the most variable RAPD patterns were obtained previously for this Schistosomatidae representative [15]. The analysis of polymorphic DNA fragments included sequence homology search using available nucleotide and amino sequences of the avian (Trichobilharzia spp.)…”

Section: Introductionmentioning

confidence: 99%

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Characterization of randomly amplified polymorphic DNA (RAPD) fragments revealing clonal variability in cercariae of avian schistosome <i>Trichobilharzia szidati</i> (Trematoda: Schistosomatidae)

Korsunenko¹,

Chrisanfova²,

Arifov³

et al. 2013

OJGen

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Recently we applied randomly amplified polymorphic DNA (RAPD) fingerprinting to detect clonal variability among individual cercariae within daughter sporocysts and rediae of 10 digenean trematodes (Platyhelminthes: Trematoda). The most variable RAPD patterns were obtained for Schistosomatidae representative-avian schistosome Trichobilharzia szidati. In this work, 50 polymorphic DNA fragments of approximately 300-1500 bp from RAPD patterns of individual T. szidati cercariae were cloned and sequenced. As a result genomic DNA sequences (total length of approximately 41,000 bp) revealing clonal variability in T. szidati cercariae were obtained and analyzed. The analysis indicated that these sequences contained tandem, inverted and dispersed repeats as well as regions homological to retroelements of two human parasites, Schistosoma mansoni and S. japonicum. Tandem and inverted repeats constituted 8.9% and 22.1% respectively, while the percentage of dispersed repeats was 21.0%. The average content of these components was 41.7% with the average AT content being 59.0%. About 40% of sequences included regions ranging in length from 96 to 1005 bp which displayed amino acid homology with open reading frame pol products of S. mansoni and S. japonicum retroelements: non-long terminal repeat retrotransposons (nLTRs, 76%), long terminal repeat retrotransposons (LTRs, 14%), and Penelope-like elements (PLEs, 10%). Most of these regions (86.4%) contained frameshifts, gaps, and stop-codons. The largest portion of them was homological to nLTRs of the RTE clade (67%). The number of sequences homologous to the members of CR1 lineage was 7 times smaller (9%). Homology with LTRs of Gypsy/Ty3 and BEL clades was revealed in 5% and 9% of cases respectively. We assume that the repetitive elements including retroelement-like sequences described in the current study may serve as the source of clonal variability detected previously in T. szidati and other digenean trematodes. Such genome regions rapidly accumulate mutations and thus may play an important functional role in the life history of the species.

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Section: Discussionsupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of randomly amplified polymorphic DNA (RAPD) fragments revealing clonal variability in cercariae of avian schistosome <i>Trichobilharzia szidati</i> (Trematoda: Schistosomatidae)

Korsunenko¹,

Chrisanfova²,

Arifov³

et al. 2013

OJGen

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“…Yin et al (2008) revealed the presence of at least nine niMLG among worms arising from monomiracidial snail infection and regarded somatic mutations as the most likely explanation of this phenomenon. Recently, based on RAPD markers, we revealed significant genetic heterogeneity between cercariae within parthenites (sporocysts) for six trematode species from Schistosomatidae, Strigeidae, Gorgoderidae, Bucephalidae, Diplostomatidae, and Plagiorchiidae families (Semyenova et al 2007). RAPD variability within daughter sporocysts was also determined in population genetic studies of some trematodes: Microphallus pygmaeus and Microphallus pseudopygmaeus (Khalturin et al 2000) and B. polymorphus (Korsunenko et al 2009).…”

Section: Discussionmentioning

confidence: 98%

Genetic differentiation of cercariae infrapopulations of the avian schistosome Trichobilharzia szidati based on RAPD markers and mitochondrial cox1 gene

et al. 2011

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Avian schistosome Trichobilharzia szidati is a member of the largest genus within the family Schistosomatidae (Trematoda). Population genetic structure of Trichobilharzia spp. schistosomes, causative agents of cercarial dermatitis in humans, has not been studied yet. The knowledge of the genetic structure of trichobilharzian populations is essential for understanding the host-parasite coevolutionary dynamics and epidemiology strategies. Here we examined genetic diversity in three geographically isolated local populations of T. szidati cercariae inhabiting Russia based on nuclear (randomly amplified polymorphic DNA, RAPD) and mt (cox1) markers. We analyzed T. szidati cercariae shed from seven naturally infected snails of Lymnaea stagnalis. Using three random primers, we demonstrated genetic variation among populations, thus posing genetic structure across geographic sites. Moreover, T. szidati cercariae have been genetically structured among hosts (infrapopulations). Molecular variance analysis was performed to test the significance of genetic differentiation within and between local populations. Of total parasitic diversity, 18.8% was partitioned between populations, whereas the higher contribution (48.9%) corresponds to the differences among individual cercariae within infrapopulations. In contrast to RAPD markers, a 1,125-bp fragment of cox1 mt gene failed to provide any significant within-species structure. The lack of geographic structuring was detected using unique haplotypes which were determined in the current work for Moscow and Western Siberian local populations as well as obtained previously for European isolates (Czech Republic and Germany). All T. szidati/Trichobilharzia ocellata haplotypes were found to be mixed across their geographical origin.

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TrematodeHimasthla elongata marinerelement (Hemar): Structure and applications

Galaktionov¹,

Solovyeva

Федоров

et al. 2013

J Exp Zool Pt B

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We cloned and analyzed Hemar1-the full-length mariner of Himasthla elongata. Hemar1 amount and distribution in the genome is typical for the transposable elements. Hemar1 closest relatives found in databases are the mariner-like element (MLE) of Girardia tigrina with 88% similarity in the most conserved transposase domain and Cemar1 of Caenorhabditis elegans with the most similar inverted terminal repeats. Hydra's (Cnidaria) MLE are the next in similarity to Hemar1. We checked whether sequences similar to Hemar1 exist in intermediate and definitive hosts of the parasitic trematode and did not find obvious similarity. This fact, together with the data of Hemar1 evolutionary position, argues against recent MLE-mediated horizontal transfer in this parasite-host model. Our results demonstrate that H. elongata generates genomic variability in asexual parthenogenetic generations within the snail. Transposon insertional display based on full-length sequence showed that Hemar1 could be located in the regions involved in generating clonal diversity in rediae and cercariae, that is, trematode parthenitae.

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Multilocus variation in cercariae, parthenogenetic progeny of different species of the class trematoda

Cited by 10 publications

References 7 publications

Characterization of randomly amplified polymorphic DNA (RAPD) fragments revealing clonal variability in cercariae of avian schistosome <i>Trichobilharzia szidati</i> (Trematoda: Schistosomatidae)

Characterization of randomly amplified polymorphic DNA (RAPD) fragments revealing clonal variability in cercariae of avian schistosome <i>Trichobilharzia szidati</i> (Trematoda: Schistosomatidae)

Genetic differentiation of cercariae infrapopulations of the avian schistosome Trichobilharzia szidati based on RAPD markers and mitochondrial cox1 gene

TrematodeHimasthla elongata marinerelement (Hemar): Structure and applications

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Multilocus variation in cercariae, parthenogenetic progeny of different species of the class trematoda

Cited by 10 publications

References 7 publications

Characterization of randomly amplified polymorphic DNA (RAPD) fragments revealing clonal variability in cercariae of avian schistosome &lt;i&gt;Trichobilharzia szidati&lt;/i&gt; (Trematoda: Schistosomatidae)

Characterization of randomly amplified polymorphic DNA (RAPD) fragments revealing clonal variability in cercariae of avian schistosome &lt;i&gt;Trichobilharzia szidati&lt;/i&gt; (Trematoda: Schistosomatidae)

Genetic differentiation of cercariae infrapopulations of the avian schistosome Trichobilharzia szidati based on RAPD markers and mitochondrial cox1 gene

TrematodeHimasthla elongata marinerelement (Hemar): Structure and applications

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Characterization of randomly amplified polymorphic DNA (RAPD) fragments revealing clonal variability in cercariae of avian schistosome <i>Trichobilharzia szidati</i> (Trematoda: Schistosomatidae)

Characterization of randomly amplified polymorphic DNA (RAPD) fragments revealing clonal variability in cercariae of avian schistosome <i>Trichobilharzia szidati</i> (Trematoda: Schistosomatidae)