Multimarker Responses of Zebrafish to the Effect of Ibuprofen and Gemfibrozil in Environmentally Relevant Concentrations

Falfushynska, Halina; Poznanskyi, Dmytro; Kasianchuk, Nadiia; Horyn, Oksana; Боднар, О. І.

doi:10.1007/s00128-022-03607-2

Cited by 6 publications

(3 citation statements)

References 36 publications

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“…To disclose the balance between antioxidants and prooxidants in zebrafish liver after exposure to studied pesticides, the integrative index of oxidative stress was calculated as IOS = ( TAC + CAT )/( ROS + TBARS + PC ) using standardized data [ 40 ]. Firstly, the deviation from the corresponding control (D(B)) was calculated for each of the selected biomarkers according to the formula D(B) = (Mean (B) experimental − Mean (B) control )/Mean (B) control .…”

Section: Methodsmentioning

confidence: 99%

Molecular and Biochemical Evidence of the Toxic Effects of Terbuthylazine and Malathion in Zebrafish

Khatib

Horyn

Боднар

et al. 2023

Animals

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Our research sought to determine the molecular and biochemical effects of environmentally relevant exposure to commonly used chloro-s-triazine herbicide terbuthylazine and organophosphate insecticide malathion on zebrafish. To this aim, mature zebrafish were exposed to 2 and 30 µg L−1 terbuthylazine and 5 and 50 µg L−1 malathion alone and in combination for 14 days. Aside from the accumulation of TBARS and protein carbonyls, a decrease in antioxidants and succinate dehydrogenase activity, an increase in oxidized glutathione, and enhanced apoptosis via Caspase-3 and BAX overexpression were observed. Furthermore, terbuthylazine and malathion induced mitochondrial swelling (up to 210% after single exposure and up to 470% after co-exposure) and lactate dehydrogenase leakage (up to 268% after single exposure and up to 570% after co-exposure) in a concentration-dependent manner. Significant upregulation of ubiquitin expression and increased cathepsin D activity were characteristics that appeared only upon terbuthylazine exposure, whereas the induction of IgM was identified as the specific characteristic of malathion toxicity. Meanwhile, no alterations in the zebrafish hypothalamic-pituitary-thyroid axis was observed. Co-exposure increased the adverse effects of individual pesticides on zebrafish. This study should improve the understanding of the mechanisms of pesticide toxicity that lead to fish impairment and biodiversity decline.

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Section: Methodsmentioning

confidence: 99%

Molecular and Biochemical Evidence of the Toxic Effects of Terbuthylazine and Malathion in Zebrafish

Khatib

Horyn

Боднар

et al. 2023

Animals

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“…METH-induced impairment of mitochondrial function might also increase susceptibility to oxidative stress, promote programmed cell death, and exacerbate neuroinflammation [ 130 ]. Our previous research has demonstrated that zebrafish mitochondria are susceptible to various stressors, and the concept of “metabolic arrest” plays a pivotal role in their survival when exposed to a wide range of organic pollutants, including pharmaceuticals and cyanotoxins [ 131 , 132 , 133 , 134 , 135 ]. Therefore, it is plausible that similar mechanisms observed in mammals may also occur in zebrafish, although experimental verification is needed.…”

Section: Bioaccumulation Of Illicit Drugs and Their Toxicitymentioning

confidence: 99%

“…Vitellogenin undergoes proteolytic cleavage, producing two lipovitellins and one phosvitin [ 145 ], essential for ensuring adequate nutrient and energy supply and immunity throughout embryo development [ 146 , 147 ]. Since changes in vitellogenin patterns are commonly associated with substances that disrupt the endocrine system [ 132 ], further research is needed to uncover the precise mechanisms underlying the endocrine-modulatory effects of IDs on fish and their implications for aquatic ecosystems. Additionally, there is evidence of widespread endocrine disruption in fish, with estrogenic effects demonstrated in various aquatic environments [ 148 ].…”

Section: Bioaccumulation Of Illicit Drugs and Their Toxicitymentioning

confidence: 99%

Illicit Drugs in Surface Waters: How to Get Fish off the Addictive Hook

Falfushynska,

Rychter,

Boshtova

et al. 2024

Pharmaceuticals

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The United Nations World Drug Report published in 2022 alarmed that the global market of illicit drugs is steadily expanding in space and scale. Substances of abuse are usually perceived in the light of threats to human health and public security, while the environmental aspects of their use and subsequent emissions usually remain less explored. However, as with other human activities, drug production, trade, and consumption of drugs may leave their environmental mark. Therefore, this paper aims to review the occurrence of illicit drugs in surface waters and their bioaccumulation and toxicity in fish. Illicit drugs of different groups, i.e., psychostimulants (methamphetamines/amphetamines, cocaine, and its metabolite benzoylecgonine) and depressants (opioids: morphine, heroin, methadone, fentanyl), can reach the aquatic environment through wastewater discharge as they are often not entirely removed during wastewater treatment processes, resulting in their subsequent circulation in nanomolar concentrations, potentially affecting aquatic biota, including fish. Exposure to such xenobiotics can induce oxidative stress and dysfunction to mitochondrial and lysosomal function, distort locomotion activity by regulating the dopaminergic and glutamatergic systems, increase the predation risk, instigate neurological disorders, disbalance neurotransmission, and produce histopathological alterations in the brain and liver tissues, similar to those described in mammals. Hence, this drugs-related multidimensional harm to fish should be thoroughly investigated in line with environmental protection policies before it is too late. At the same time, selected fish species (e.g., Danio rerio, zebrafish) can be employed as models to study toxic and binge-like effects of psychoactive, illicit compounds.

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Nb2O5/MWCNT nanocomposites for the degradation of ibuprofen via photocatalysis and catalytic ozonation

Fidelis,

Santos,

Paula

et al. 2024

Catalysis Communications

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Multimarker Responses of Zebrafish to the Effect of Ibuprofen and Gemfibrozil in Environmentally Relevant Concentrations

Cited by 6 publications

References 36 publications

Molecular and Biochemical Evidence of the Toxic Effects of Terbuthylazine and Malathion in Zebrafish

Molecular and Biochemical Evidence of the Toxic Effects of Terbuthylazine and Malathion in Zebrafish

Illicit Drugs in Surface Waters: How to Get Fish off the Addictive Hook

Nb2O5/MWCNT nanocomposites for the degradation of ibuprofen via photocatalysis and catalytic ozonation

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