Multimedia distributions, bioaccumulation, and trophic transfer of microcystins in the Geum River Estuary, Korea: Application of compound-specific isotope analysis of amino acids

Kim, Do Kyun; Hong, Seongjin; Choi, Hyuntae; Choi, Bong Kyu; Kim, Jae-Seong; Khim, Jong Seong; Park, Hodong; Shin, Kyung-Hoon

doi:10.1016/j.envint.2019.105194

Cited by 27 publications

(10 citation statements)

References 57 publications

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“…At the most downstream estuarine site MCs were detected mostly dissolved in the water, consistent with the increased cell lysis caused by high sanility [28]. Kim et al (2019) [29] reported great amounts of MCs (concentrations varying from 0.4 to 75 µgL −1 ) in Geum River Estuary, Korea, associated with increased freshwater discharges during the rainy season. Accumulation of MCs was reported in several marine organisms, but was higher in organisms of lower trophic positions.…”

Section: Introductionmentioning

confidence: 59%

Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

Oliveira

Díez-Quijada

Turkina

et al. 2020

Toxins

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Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species are affected by the exposure to freshwater cyanobacteria and cyanotoxins. So far, most of the knowledge has been gathered from freshwater bivalve molluscs. This work aimed to infer the sensitivity of the marine mussel Mytilus galloprovincialis to single as well as mixed toxic cyanobacterial cultures and the underlying molecular responses mediated by toxic cyanobacteria. For this purpose, a mussel exposure experiment was outlined with two toxic cyanobacteria species, Microcystis aeruginosa and Chrysosporum ovalisporum at 1 × 105 cells/mL, resembling a natural cyanobacteria bloom. The estimated amount of toxins produced by M. aeruginosa and C. ovalisporum were respectively 0.023 pg/cell of microcystin-LR (MC-LR) and 7.854 pg/cell of cylindrospermopsin (CYN). After 15 days of exposure to single and mixed cyanobacteria, a depuration phase followed, during which mussels were fed only non-toxic microalga Parachlorella kessleri. The results showed that the marine mussel is able to filter toxic cyanobacteria at a rate equal or higher than the non-toxic microalga P. kessleri. Filtration rates observed after 15 days of feeding toxic microalgae were 1773.04 mL/ind.h (for M. aeruginosa), 2151.83 mL/ind.h (for C. ovalisporum), 1673.29 mL/ind.h (for the mixture of the 2 cyanobacteria) and 2539.25 mL/ind.h (for the non-toxic P. kessleri). Filtering toxic microalgae in combination resulted in the accumulation of 14.17 ng/g dw MC-LR and 92.08 ng/g dw CYN. Other physiological and biochemical endpoints (dry weight, byssus production, total protein and glycogen) measured in this work did not change significantly in the groups exposed to toxic cyanobacteria with regard to control group, suggesting that mussels were not affected with the toxic microalgae. Nevertheless, proteomics revealed changes in metabolism of mussels related to diet, specially evident in those fed on combined cyanobacteria. Changes in metabolic pathways related with protein folding and stabilization, cytoskeleton structure, and gene transcription/translation were observed after exposure and feeding toxic cyanobacteria. These changes occur in vital metabolic processes and may contribute to protect mussels from toxic effects of the toxins MC-LR and CYN.

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Section: Introductionmentioning

confidence: 59%

Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

Oliveira

Díez-Quijada

Turkina

et al. 2020

Toxins

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“…21 Importantly, timedependent accumulation of MC-LR in the lungs was found in addition to dose-dependent accumulation. MC-LR is widely distributed in the environment and can be enriched and transferred through the food chain; 34 hence, the potential threat posed by long-term MC-LR exposure to human and animal health cannot be ignored.…”

Section: Discussionmentioning

confidence: 99%

Chronic Exposure to Environmentally Relevant Concentrations of Microcystin-Leucine Arginine Causes Lung Barrier Damage through PP2A Activity Inhibition and Claudin1 Ubiquitination

Liu

Zeng

Wang

et al. 2022

J. Agric. Food Chem.

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Microcystin-leucine arginine (MC-LR), ubiquitous in water and food, is a threat to public health. In the present study, after C57BL/6J mice were fed with environmental concentrations of MC-LR (0, 1, 30, 60, 90, and 120 μg/L) for 6, 9, and 12 months, it was found that MC-LR could enter into mouse lung tissues and cause microstructural damage, as shown by western blotting and HE staining. Electron microscopy examination showed that MC-LR could damage the lung barrier by disruption of the tight junctions, which was confirmed by the decreased expression of tight junction markers, including Occludin, Claudin1, and ZO-1. In addition, MC-LR also increased the ubiquitination of Claudin1, indicating that MC-LR could disrupt tight junctions by promoting the degradation of Claudin1. Furthermore, MC-LR increased the levels of TNF-α and IL-6 in mouse lung tissues, leading to pneumonia. Importantly, pretreatment with PP2A activator D-erythro-sphingosine (DES) was found to significantly alleviate MC-LR-induced decrease of Occludin and Claudin1 by inhibiting the P-AKT/Snail pathway in vitro. Together, this study revealed that chronic exposure to MC-LR causes lung barrier damage, which involves PP2A activity inhibition and enhancement of Claudin1 ubiquitination. This study broadens the awareness of the toxic effects of MC-LR on the respiratory system, which has deep implications for public health.

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“…This reinforces the hypothesis that contamination of mussels with MCs occurs mainly through the filtration of cyanobacteria flowing downstream with differential accumulation kinetics between variants, probably influenced by environmental parameters such as the salinity; a hypothesis that should be further assessed. Similarly, Kim et al (2019;2021) showed that the concentration of MC-RR was lower than MC-LR and MC-YR in clams, worms, crabs, and fish from the Geum river estuary in Korea. Interestingly, in the present study, marine mussels accumulated less MC-RR but more dmMC-RR than was available in food.…”

Section: Variant Profiles In Phytoplankton and Bivalves Of Estuarine Sitesmentioning

confidence: 94%

“…Most studies on cyanobacteria and cyanotoxin occurrence were conducted in freshwater ecosystems, but intermittent cyanobacteria and MC transfer during a freshwater discharge into the coastal environment were recently reported in California (Gibble and Kudela, 2014), Italy (De Pace et al, 2014), Japan (Umehara et al, 2015), Washington state (Preece et al, 2015;, Korea (Kim et al, 2019), France (Bormans et al, 2019), Lithuania (Overlinge et al, 2020) and Uruguay (Kruk et al, 2021). The resulting MC contamination of marine organisms such as molluscs, crustaceans, fish and even sea otters, has been reported in several countries (Lehman et al, 2010;Miller et al, 2010;Gibble et al, 2016;Tatters et al 2017;Peacock et al, 2018;Kim et al, 2019), and could have a large health and economic impact. However, the contamination level in organisms consumed by humans may have been underestimated because only the free (not protein-bound) fraction of MC accumulation is routinely quantified in tissues.…”

Section: Introductionmentioning

confidence: 99%

In situ use of bivalves and passive samplers to reveal water contamination by microcystins along a freshwater-marine continuum in France

et al. 2021

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Cyanobacteria are a potential threat to aquatic ecosystems and human health because of their ability to produce cyanotoxins, such as microcystins (MCs). MCs are regularly monitored in fresh waters, but rarely in estuarine and marine waters despite the possibility of their downstream export. Over a period of two years, we monthly analysed intracellular (in phytoplankton) and extracellular (dissolved in water) MCs at five stations along a river continuum from a freshwater reservoir with ongoing cyanobacterial blooms to the coast of Brittany, France. MCs were quantified using two integrative samplers placed at each site: solid phase adsorption toxin tracking (SPATT) samplers for collecting extracellular MCs and caged mussels (Anodonta anatina and Mytilus edulis) filter-feeding on MC-producing cyanobacteria.The MC transfer was demonstrated each year during five months at estuarine sites and sporadically at the marine outlet. SPATT samplers integrated extracellular MCs, notably at low environmental concentrations (0.2 µg/L) and with the same variant profile as in water. The mussel A. anatina highlighted the presence of MCs including at intracellular concentrations below 1 µg/L. M. edulis more efficiently revealed the MC transfer at estuarine sites than punctual water samplings. Bivalves showed the same MC variant profile as phytoplankton samples, but with differential accumulation capacities between the variants and the two species.Using SPATT or bivalves can give a more accurate assessment of the contamination level of a freshwater-marine continuum, in which the MC transfer can be episodic. MC content in M. edulis represents a potent threat to human health if considering updated French guideline values, and particularly the total (free and protein-bound) MC content, highlighting the necessity to include cyanotoxins in the monitoring of seafood originating from estuarine areas.

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Multimedia distributions, bioaccumulation, and trophic transfer of microcystins in the Geum River Estuary, Korea: Application of compound-specific isotope analysis of amino acids

Cited by 27 publications

References 57 publications

Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

Chronic Exposure to Environmentally Relevant Concentrations of Microcystin-Leucine Arginine Causes Lung Barrier Damage through PP2A Activity Inhibition and Claudin1 Ubiquitination

In situ use of bivalves and passive samplers to reveal water contamination by microcystins along a freshwater-marine continuum in France

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