Multimeric and trimeric subunit SP-D are interconvertible structures with distinct ligand interaction

Sørensen, Grith Lykke; Hoegh, Silje Vermedal; Leth-Larsen, Rikke; Thomsen, Theresa; Floridon, Charlotte; Smith, Kenneth Michael; Kejling, Karin; Tornøe, Ida; Crouch, Erika C.; Holmskov, Uffe

doi:10.1016/j.molimm.2009.06.005

Cited by 32 publications

(48 citation statements)

References 25 publications

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“…In our studies, using unreduced protein preparations, the anti-SP-D antibodies recognized proteins of w120 and 180 kDa (see Fig. 3A, lane 3) consistent with previous reports (Sørensen et al 2009). Interestingly, in murine islets, a single band of w100 kDa was detected and in the nonreduced state, a band of w180 kDa was observed (Fig.…”

Section: A Potential Role Of Sp-d In Pancreatic B Cellssupporting

confidence: 92%

“…3A, lane 2, at w40-45 kDa) but not in the brain (lane 1), consistent with the known pattern of SP-D expression (Motwani et al 1995). The immunoreactive SP-D detected in mouse lung was consistent with monomeric SP-D, which is w45 kDa (Sørensen et al 2009). However, SP-D is commonly present in large complex structures (Sørensen et al 2009).…”

Section: A Potential Role Of Sp-d In Pancreatic B Cellsmentioning

confidence: 61%

“…The immunoreactive SP-D detected in mouse lung was consistent with monomeric SP-D, which is w45 kDa (Sørensen et al 2009). However, SP-D is commonly present in large complex structures (Sørensen et al 2009). In unreduced preparations, the antibody typically recognizes proteins with a molecular weight of w120 kDa and above, which likely represent trimers and other multimers.…”

Section: A Potential Role Of Sp-d In Pancreatic B Cellsmentioning

confidence: 61%

“…For SP-D samples, both reduced and nonreduced protein samples were examined. For reduced samples for SP-D, protein preparations were prepared by mixing the protein samples 1:2 with Laemmli sample buffer with 5% 2-mercaptoethanol and heating at 100 8C for 1 min (nonreduced preparations were heated in sample buffer with the addition of iodoacetamide, final concentration of 90 mM) as previously described (Madsen et al 2000, Sørensen et al 2009). Each assay was repeated with two or three independent samples.…”

Section: Western Blotmentioning

confidence: 99%

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Regulation of pancreatic islet gene expression in mouse islets by pregnancy

Layden

Durai²,

Newman³

et al. 2010

Journal of Endocrinology

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Pancreatic b cells adapt to pregnancy-induced insulin resistance by unclear mechanisms. This study sought to identify genes involved in b cell adaptation during pregnancy. To examine changes in global RNA expression during pregnancy, murine islets were isolated at a time point of increased b cell proliferation (E13 . 5), and RNA levels were determined by two different assays (global gene expression array and G-protein-coupled receptor (GPCR) array). Follow-up studies confirmed the findings for select genes. Differential expression of 110 genes was identified and follow-up studies confirmed the changes in select genes at both the RNA and protein level. Surfactant protein D (SP-D) mRNA and protein levels exhibited large increases, which were confirmed in murine islets. Cytokine-induced expression of SP-D in islets was also demonstrated, suggesting a possible role as an anti-inflammatory molecule. Complementing these studies, an expression array was performed to define pregnancy-induced changes in expression of GPCRs that are known to impact islet cell function and proliferation. This assay, the results of which were confirmed using realtime reverse transcription-PCR assays, demonstrated that free fatty acid receptor 2 and cholecystokinin receptor A mRNA levels were increased at E13 . 5. This study has identified multiple novel targets that may be important for the adaptation of islets to pregnancy.

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Section: A Potential Role Of Sp-d In Pancreatic B Cellssupporting

confidence: 92%

Section: A Potential Role Of Sp-d In Pancreatic B Cellsmentioning

confidence: 61%

Section: A Potential Role Of Sp-d In Pancreatic B Cellsmentioning

confidence: 61%

Section: Western Blotmentioning

confidence: 99%

See 2 more Smart Citations

Regulation of pancreatic islet gene expression in mouse islets by pregnancy

Layden

Durai²,

Newman³

et al. 2010

Journal of Endocrinology

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“…The significance of the Met31Thr polymorphism is probably due to its influence on the binding of pathogen-associated molecular patterns. Indeed, highly multimerized and lower-molecular-weight forms of SP-D have been shown to exhibit differential binding to microbial ligands, with the former preferentially binding mannan and lipoteichoic acid and the latter, lipopolysaccharide and peptidoglycan (13,19). In the Finnish population, term-born young children carrying the SFTPD codon 31 methionine allele were shown to be predisposed to severe respiratory syncytial virus infection (20).…”

Section: Articlesmentioning

confidence: 99%

A study of collectin genes in spontaneous preterm birth reveals an association with a common surfactant protein D gene polymorphism

et al. 2011

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ArticlesClinical Investigation nature publishing group INTRODUCTION: Preterm birth is the major cause of mortality and morbidity in neonates. Intrauterine infection and/or inflammatory response are evident in 60-70% of spontaneous preterm births (sPTBs). Genetic factors significantly increase this risk. however, the genetic background associated with sPTB is poorly understood. surfactant protein (sP) a, sP-D, and mannose-binding lectin (MBL) are structurally and functionally related collectins that bind pathogen-associated molecular patterns, and mostly suppress innate immune responses. RESULTS:We detected an overrepresentation of the methionine allele of the SFTPD gene (encoding sP-D) Met31Thr polymorphism in preterm infants as compared to term infants. This association was highly significant in infants of families with recurrent sPTBs (P = 0.001, odds ratio = 1.65, 95% confidence interval = 1.22-2.22); however, there was no such association with SFTPD in the mothers of these infants. Polymorphism of the genes encoding sP-a and MBL did not influence the risk of sPTB. DISCUSSION: Our results suggest that the fetal SFTPD Met31Thr polymorphism plays a significant role in genetic predisposition to sPTB. We propose that fetal immune responses influence sensitivity to preterm labor-inducing signals. METHODS: Genes encoding sP-a, sP-D, and MBL were investigated as potential candidates for association with sPTB in a population of preterm singleton infants (n = 406) and their mothers (n = 308), and in mothers with term deliveries (n = 201) and their infants (n = 201), all originating from northern Finland.

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Surfactant Protein-D

Gupta

2012

Animal Lectins: Form, Function and Clinical Applications

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Multimeric and trimeric subunit SP-D are interconvertible structures with distinct ligand interaction

Cited by 32 publications

References 25 publications

Regulation of pancreatic islet gene expression in mouse islets by pregnancy

Regulation of pancreatic islet gene expression in mouse islets by pregnancy

A study of collectin genes in spontaneous preterm birth reveals an association with a common surfactant protein D gene polymorphism

Surfactant Protein-D

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