Multimodal, Biomaterial‐Focused Anticoagulation via Superlow Fouling Zwitterionic Functional Groups Coupled with Anti‐Platelet Nitric Oxide Release

Amoako, Kagya A.; Sundaram, Harihara S.; Suhaib, Ahmed B.; Jiang, Shaoyi; Cook, Keith E.

doi:10.1002/admi.201500646

Cited by 36 publications

(29 citation statements)

References 42 publications

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“…35, 41–61 It is a free radical that reacts with superoxides and oxygen to form peroxynitrite and dinitrogen trioxide, respectively. 42, 43, 62 Nitric oxide’s mechanisms of action against bacteria include nitrosation of amines and thiols in the extracellular matrix, lipid peroxidation and tyrosine nitration in the cell wall, and DNA cleavage in the cellular matrix.…”

Section: Introductionmentioning

confidence: 99%

Enhanced antibacterial efficacy of nitric oxide releasing thermoplastic polyurethanes with antifouling hydrophilic topcoats

et al. 2017

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Surface fouling is one of the leading causes of infection associated with implants, stents, catheters, and other medical devices. The surface chemistry of medical device coatings is important in controlling and/or preventing fouling. In this study, we have shown that a combination of nitric oxide releasing hydrophobic polymer with a hydrophilic polymer topcoat can significantly reduce protein attachment and subsequently reduce bacterial adhesion as a result of the synergistic effect. Nitric oxide (NO) is a well-known potent antibacterial agent due to its adverse reactions on microbial cell components. Owing to the surface chemistry of hydrophilic polymers, they are suitable as antifouling topcoats. In this study, four biomedical grade polymers were compared for protein adhesion and NO-release behavior: CarboSil 2080A, RTV, SP60D60, and SG80A. SP60D60 was found to resist protein adsorption up to 80% when compared to the other polymers while CarboSil 2080A maintained a steady NO flux even after 24 hours (~0.50 × 10−10 mol cm−2 min−1) of soaking in buffer solution with a loss of less than 3 wt% S-nitroso-N-acetylpenicillamine (SNAP), the NO donor molecule, in the leaching analysis. Therefore, CarboSil 2080A incorporated with SNAP and topcoated with SP60D60, was tested for antibacterial efficacy after exposure to fibrinogen, an abundantly found protein in blood. The NO-releasing CarboSil 2080A with SP60D60 topcoated polymer showed a 96% reduction in Staphylococcus aureus viable cell count compared to the control samples. Hence, the study demonstrated that a hydrophilic polymer topcoat, when applied to a polymer with sustained NO release from underlying SNAP incorporated hydrophobic polymer, can reduce bacterial adhesion and be used as a highly efficient antifouling, antibacterial polymer for biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Enhanced antibacterial efficacy of nitric oxide releasing thermoplastic polyurethanes with antifouling hydrophilic topcoats

et al. 2017

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“…However, in addition to the small capillary diameter, interaction of blood components such as platelets with exposed PDMS may cause clogging of the capillaries. Recent developments of antifouling coatings (also dopamine‐based) could help circumventing this issue as these coatings drastically reduce the interactions of blood platelets and fibrinogen and PDMS …”

Section: Discussionmentioning

confidence: 99%

“…Recent developments of antifouling coatings (also dopamine-based) could help circumventing this issue as these coatings drastically reduce the interactions of blood platelets and fibrinogen and PDMS. [54] Alternative applications of this multifunctional device could involve studying the binding interactions of immobilized biomacromolecules such as mucins with a set of smaller test molecules as well as the immobilization of enzymes, thus providing a low-cost stationary phase for performing enzymatic reactions in a laboratory scale without the need of separating the enzymes from the substrates afterward.…”

Section: Wwwadvancedsciencenewscom Wwwmbs-journaldementioning

confidence: 99%

Macromolecular Coating Enables Tunable Selectivity in a Porous PDMS Matrix

Winkeljann

Käsdorf

Boekhoven

et al. 2017

Macromolecular Bioscience

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Whether for laboratory use or clinical practice, many fields in Life Sciences require selective filtering. However, most existing filter systems lack the ability to easily tune their filtration behavior. Two key elements for efficient filtering are a high surface-to-volume ratio and the presence of suitable chemical groups which establish selectivity. In this study, an artificial PDMS-based capillary system with highly tunable selectivity properties is presented. The high surface-to-volume ratio of this filter system is generated by first embedding sugar fibers into a synthetic polymer matrix and then dissolving these fibers from the cured polymer. To functionalize this filter, the inner surface of the capillaries is coated with purified or synthetic macromolecules. Depending on the type of macromolecule used for filter functionalization, selective sieving is observed based on steric hindrance, electrostatic binding, electrostatic repulsion, or specific binding interactions. Furthermore, it is demonstrated that enzymes can be immobilized in the capillary system which allows for performing multiple cycles of enzymatic reactions with the same batch of enzymes and without the need to separate the enzymes from their reaction products. In addition to lab-scale filtration and enzyme immobilization applications demonstrated here, the functionalized porous PDMS matrix may also be used to test binding interactions between different molecules.

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Section: Introductionmentioning

confidence: 99%

“…Incorporation of hydrophilic functional groups to a polymeric material has, in recent years, been considered an effective method of improving resistance to protein adsorption and reducing the coagulant properties of membranes. 16,17 Carboxyl and hydroxyl groups are two hydrophilic functional groups. Our previous study 18 demonstrated that introduction of these two functional groups could effectively achieve these two objectives for a biomaterial surface that contacts blood.…”

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confidence: 99%

Improvement in the blood compatibility of polyvinylidene fluoride membranes via in situ cross‐linking polymerization

Nie

Zeng

Qin

et al. 2018

Polymers for Advanced Techs

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In this study, we have provided a highly efficient, convenient, and universal protocol for preparing polyvinylidene fluoride (PVDF) membranes with low blood contact activation via in situ cross‐linking copolymerization of 2‐hydroxyethl methacrylate (HEMA) and acrylic acid (AA) in a solution of PVDF. The modified membranes were prepared from PVDF solution by phase inversion technology. The composition and morphology of the modified membranes were confirmed by attenuated total reflectance‐Fourier transform infrared (ATR‐FTIR) spectroscopy, thermogravimetric (TG) analysis, and scanning electron microscopy (SEM). Protein adsorption, clotting time, and contact activation on the modified PVDF membranes were systematically studied, the results indicating that after the incorporation of AA and HEMA, the modified PVDF membranes possessed anticoagulant properties in addition to low contact activation of blood components when in contact with blood. Therefore, fluorinated PVDF membranes with surfaces enriched with carboxyl and hydroxyl groups possessed the potential for use in long‐term blood‐contacting devices.

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Multimodal, Biomaterial‐Focused Anticoagulation via Superlow Fouling Zwitterionic Functional Groups Coupled with Anti‐Platelet Nitric Oxide Release

Cited by 36 publications

References 42 publications

Enhanced antibacterial efficacy of nitric oxide releasing thermoplastic polyurethanes with antifouling hydrophilic topcoats

Enhanced antibacterial efficacy of nitric oxide releasing thermoplastic polyurethanes with antifouling hydrophilic topcoats

Macromolecular Coating Enables Tunable Selectivity in a Porous PDMS Matrix

Improvement in the blood compatibility of polyvinylidene fluoride membranes via in situ cross‐linking polymerization

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