Multimodal control of pituitary gonadotropin biosynthesis. Effects of gonadal steroids, GnRH, cyclicAMP and diacylglycerols

Counis, Raymond; Starzec, Anna; Corbani, Maïthé; Jutisz, M

doi:10.1051/rnd:19880714

Cited by 2 publications

(3 citation statements)

References 21 publications

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“…Longer expo sure of cells to low concentrations of GnRH, and priming of cells with sex steroids-containing sera or estrogenic phenol red may increase mRNA levels for LH(5 subunit (41], and stimulate synthesis of the polypeptide chains of LH [2,9,48]. …”

Section: Discussionmentioning

confidence: 99%

17-Beta-Estradiol Potentiates Luteinizing Hormone Glycosylation and Release Induced by Veratridine, Diacylglycerol, and Phospholipase C in Rat Anterior Pituitary Cells

Liu

Jackson²

1990

Neuroendocrinology

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We determined the effect of 17β-estradiol (E₂) on synthesis and release of luteinizing hormone (LH) induced by drugs which activate intracellular signal transduction mechanisms in rat anterior pituitary cells. Cells were pretreated with E₂ (6 × 10^–10M) or diluent for 24 h, then washed and incubated for 4 h with E₂ or diluent, respectively, in the presence or absence of drugs. LH translation and glycosylation were monitored by measuring incorporation of [¹⁴C]alanine and [³H]glucosamine, respectively, into total (medium plus cells) immunoprecipitable LH. Immunoreactive LH (IRLH) was measured by radioimmunoassay. Gonadotropin-releasing hormone (GnRH, 1 ILM), veratridine (5 µM), L-α-1,2-dioctanoyl glycerol (C8, 200 µM), and phospholipase C (PLC, 0.24 U/ml) all increased (p < 0.01) medium IRLH, [³H]glucosamine-LH, and [¹⁴C]alanine-LH, and total [³H]glucosamine-LH in both E₂- and diluent-treated cells. Total IRLH or [¹⁴C]alanine-LH were not increased by any treatment. E₂ alone slightly increased (p < 0.05) basal medium IRLH and [³H]glucosamine-LH. The stimulatory effects of E₂ on basal medium [¹⁴C]alanine-LH and total [³H]glucosamine-LH were inconsistent. E₂ potentiated (p < 0.01) the effects of veratridine, C8, PLC, and GnRH on medium IRLH, and medium and total [³H]glucosamine-LH. E₂ also potentiated (p < 0.01) the effects of veratridine, PLC, and GnRH, but not of C8, on medium [¹⁴C]alanine-LH. In contrast, E₂ did not increase either precursor uptake or incorporation of precursor into total protein in the presence of any secretagogue. These results suggest that LH glycosylation, like LH release, may involve the Ca²⁺ and PLC pathways, and that E₂ augments LH glycosylation and release by interacting with these pathways.

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Section: Discussionmentioning

confidence: 99%

17-Beta-Estradiol Potentiates Luteinizing Hormone Glycosylation and Release Induced by Veratridine, Diacylglycerol, and Phospholipase C in Rat Anterior Pituitary Cells

Liu

Jackson²

1990

Neuroendocrinology

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“…Estrogen's control on GnRH and LH is expressed at both the hypothalamic and pituitary level. The mechanisms that interact in order to increase or decrease estrogens control on GnRH are very complex and involve the interaction of progesterone (Counis et al, 1988) and other steroid hormones. At the hypothalamic level there are several ways in which estrogen has been proposed to control GnRH release.…”

Section: Hormonal Factors Affecting Gnrh Releasementioning

confidence: 99%

“…This mechanism is especially important in initiation of the LH surge. Also, estrogen and progesterone are known to effect the synthesis of LH and FSH in the pituitary gonadotrophs (Counis et al, 1988).…”

Section: Hormonal Factors Affecting Gnrh Releasementioning

confidence: 99%

Impact of amino acid nutrition during lactation on subsequent reproductive function of primiparous sows

Jones

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sows was entirely accounted for by body fet loss in that these sows actually accrued body protein, water, and ash. Muscle myofibrillar breakdown rate was higher (P < .01) in LP sows than HP sows (4.05 versus 2.80 %/d). Based on these data dietary amino acid restriction during lactation reduces milk nutrient output and increases maternal body tissue mobilization. Maternal protein loss is maintained over the entire lactation even though milk output is decreased.

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Multimodal control of pituitary gonadotropin biosynthesis. Effects of gonadal steroids, GnRH, cyclicAMP and diacylglycerols

Abstract: Summary. We Introduction.

Cited by 2 publications

References 21 publications

17-Beta-Estradiol Potentiates Luteinizing Hormone Glycosylation and Release Induced by Veratridine, Diacylglycerol, and Phospholipase C in Rat Anterior Pituitary Cells

17-Beta-Estradiol Potentiates Luteinizing Hormone Glycosylation and Release Induced by Veratridine, Diacylglycerol, and Phospholipase C in Rat Anterior Pituitary Cells

Impact of amino acid nutrition during lactation on subsequent reproductive function of primiparous sows

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