Multimodal electrophoresis of gold nanoparticles: A real time approach

Patra, Hirak K.; GuhaSarkar, Dwijit; Dasgupta, Anjan Kr.

doi:10.1016/j.aca.2009.07.012

Cited by 20 publications

(16 citation statements)

References 29 publications

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“…The dependence of μ e on D h −1 was earlier noted by Parak and co-workers, who showed that the mobility of DNA-AuNP depends inversely on the length of the DNA sequence bound to AuNP. 47 Similarly, mobility was also shown to be dependent on D h −1 for citrate-stabilized AuNP 48 and for BSPP-coated AuNP. 49 The reduction of μ e upon addition of drug to 1ab/ 2ab-AuNP provides additional evidence of drug loading on the particle.…”

Section: ■ Discussionmentioning

confidence: 94%

Investigation of the Drug Binding Properties and Cytotoxicity of DNA-Capped Nanoparticles Designed as Delivery Vehicles for the Anticancer Agents Doxorubicin and Actinomycin D

2012

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Oligonucleotide-functionalized gold nanoparticles (AuNP) were designed and synthesized to be delivery vehicles for the clinically used anticancer drugs doxorubicin (DOX) and actinomycin D (ActD). Each vehicle contains a tailorable number of DNA duplexes, each possessing three high-affinity sequences for the intercalation of either DOX or ActD, thus allowing for control of drug loading. Drug binding was evaluated by measuring changes to DNA melting temperature, T(m), hydrodynamic diameter, D(h), and surface plasmon resonance wavelength, λ(spr), with drug loading. These studies indicate that DOX intercalates at its high-affinity sequence bound at the AuNP, and that ActD exhibits relatively weaker binding to its preferred sequence. Agarose gel electrophoresis further confirmed drug binding and revealed that particle mobilities inversely correlate with D(h). The equilibrium binding constant, K, and dissociation rate constant, β, were determined by dialysis. Results indicate that the high negative electrostatic potential within the DNA shell of the particle significantly decreases β and enhances K for DOX but has little effect on K and β for ActD. The cytotoxicity of the vehicles was studied, with IC(50) = 5.6 ± 1.1 μM and 46.4 ± 9.3 nM for DOX-DNA-AuNP and IC(50) = 0.12 ± 0.07 μM and 0.76 ± 0.46 nM for ActD-DNA-AuNP, in terms of drug and particle concentrations, respectively.

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Section: ■ Discussionmentioning

confidence: 94%

Investigation of the Drug Binding Properties and Cytotoxicity of DNA-Capped Nanoparticles Designed as Delivery Vehicles for the Anticancer Agents Doxorubicin and Actinomycin D

2012

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“…21 In brief, an aqueous solution of HAuCl 4 (250 μM, 25 mL) was brought to boiling at 100°C and stirred continuously with a stirrer. Freshly prepared trisodium citrate solution (stock 600 μM) was added quickly all at once, with different concentrations depending on the requirement of the particle size, resulting in a change in solution color from pale yellow to deep red in the case of smaller nanoparticles.…”

Section: Gnp Preparationmentioning

confidence: 99%

“…In the case of Lys and Arg, 0.1 mM final concentration applied because of the lower stability at high amino acid concentrations. 21 The vincristine sulfate (VS)-conjugated nanoparticles were prepared similarly by replacing the amino acid with VS. 22 Cell culture Human epithelial cervical cancer (HeLa), human adenocarcinoma alveolar basal epithelial (A549), human adenocarcinoma non-small cell lung cancer (NCI H23), and human skin malignant melanoma (A375) cell lines were obtained from the National Centre for Cell Science. The cell lines were routinely grown in appropriate medium (Dulbecco's minimal essential medium-RPMI) with 10% heat-inactivated FBS (GIBCO-BRL) and adequate penicillin-streptomycin (GIBCO-BRL) at 37°C in a humidified animal cell tissue culture incubator with 5% CO 2 .…”

Section: Surface Conjugationsmentioning

confidence: 99%

Cancer cell response to nanoparticles: criticality and optimality

Dasgupta

2012

Nanomedicine: Nanotechnology, Biology and Medicine

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“…These include nanocrystals [21–26], single-walled nanotubes [27–29] and fullerenes [30]. In each case, an ionic head group of an amphiphile introduces a surface charge on a nanostructure with consequent electrostatic double-layer sufficient for the separation under aqueous electrophoretic conditions.…”

Section: Introductionmentioning

confidence: 99%

Characterization of CdSe nanocrystals coated with amphiphiles. A capillary electrophoresis study

2011

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We have synthesized CdSe nanocrystals (NCs) possessing a trioctylphosphine surface passivation layer and modified with amphiphilic molecules to form a surface bilayer. The NCs covered with single amphiphiles are not stable in aqueous solution, but a mixed amphiphilic system is shown to provide stability in solution over several months. The solutions of the modified NCs were characterized by UV-Vis absorbance, photoluminescence, and transmission electron microscopy. An electrophoretic study revealed two operational modes. The first relies on the enrichment of NCs using a micellar plug as a tool. The accumulation of NCs at the plug-electrolyte buffer interface results in a sharp peak. By controlling the electrophoretic conditions, nanocrystals were forced to exit a micellar plug into an electrolyte buffer. We conclude that a system consisting of modified nanocrystals and a micellar plug can act as a mixed pseudomicellar system, where modified nanocrystals play the role of pseudomicelles.FigureElectrophoretic focusing of amphiphile coated CdSe nanocrystals using a micellar plug.Electronic supplementary materialThe online version of this article (doi:10.1007/s00604-011-0727-8) contains supplementary material, which is available to authorized users.

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Multimodal electrophoresis of gold nanoparticles: A real time approach

Cited by 20 publications

References 29 publications

Investigation of the Drug Binding Properties and Cytotoxicity of DNA-Capped Nanoparticles Designed as Delivery Vehicles for the Anticancer Agents Doxorubicin and Actinomycin D

Investigation of the Drug Binding Properties and Cytotoxicity of DNA-Capped Nanoparticles Designed as Delivery Vehicles for the Anticancer Agents Doxorubicin and Actinomycin D

Cancer cell response to nanoparticles: criticality and optimality

Characterization of CdSe nanocrystals coated with amphiphiles. A capillary electrophoresis study

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