Multimodal fiber source for nonlinear microscopy based on a dissipative soliton laser

Lamb, Erin S.; Wise, Frank W.

doi:10.1364/boe.6.003248

Cited by 18 publications

(3 citation statements)

References 33 publications

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“…At the modulation frequency for SRS imaging, i.e., 20 MHz, the pump and Stokes beams of our fibre laser have similar noise levels, i.e., approximately −147 and −148 dBc/Hz, respectively. Note that this performance characteristic distinguishes our laser source from other designs, where one of the two-colour beams usually exhibits degraded noise performance after the nonlinear conversion processes 56,57 ; see Supplementary Material 6. Here, these two-colour beams exhibit equally low RIN levels, since the RIN of the pump beam has been improved by 50 dB compared with previous implementations (Table 1)-a key requirement for high-quality SRS imaging without the need for balanced detection 32,35 .…”

Section: Resultsmentioning

confidence: 99%

High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser

et al. 2020

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Coherent Raman scattering (CRS) microscopy is widely recognized as a powerful tool for tackling biomedical problems based on its chemically specific label-free contrast, high spatial and spectral resolution, and high sensitivity. However, the clinical translation of CRS imaging technologies has long been hindered by traditional solid-state lasers with environmentally sensitive operations and large footprints. Ultrafast fibre lasers can potentially overcome these shortcomings but have not yet been fully exploited for CRS imaging, as previous implementations have suffered from high intensity noise, a narrow tuning range and low power, resulting in low image qualities and slow imaging speeds. Here, we present a novel high-power self-synchronized two-colour pulsed fibre laser that achieves excellent performance in terms of intensity stability (improved by 50 dB), timing jitter (24.3 fs), average power fluctuation (<0.5%), modulation depth (>20 dB) and pulse width variation (<1.8%) over an extended wavenumber range (2700-3550 cm −1). The versatility of the laser source enables, for the first time, high-contrast, fast CRS imaging without complicated noise reduction via balanced detection schemes. These capabilities are demonstrated in this work by imaging a wide range of species such as living human cells and mouse arterial tissues and performing multimodal nonlinear imaging of mouse tail, kidney and brain tissue sections by utilizing second-harmonic generation and twophoton excited fluorescence, which provides multiple optical contrast mechanisms simultaneously and maximizes the gathered information content for biological visualization and medical diagnosis. This work also establishes a general scenario for remodelling existing lasers into synchronized two-colour lasers and thus promotes a wider popularization and application of CRS imaging technologies.

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Section: Resultsmentioning

confidence: 99%

High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser

et al. 2020

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“…The fast development of ultrashort pulsed laser systems has attracted a growing interest in recent years because of numerous potential scientific and industrial applications, such as micro-machining [1], frequency comb spectroscopy [2,3], precision metrology [4], Terahertz or supercontinuum generation [5][6][7], and nonlinear microscopy [8,9]. High efficiency, low cost, maintenance-free and compact sizes are the highly desirable advantages of ultrashort fiber lasers compared with bulky solid-state Ti:sapphire lasers [10].…”

Section: Introductionmentioning

confidence: 99%

Low-repetition-rate, high pulse energy all-polarization-maintaining Er-doped fiber laser mode-locked with a nonlinear amplifying loop mirror

Sun

Zhou

et al. 2019

Laser Phys. Lett.

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We demonstrate a low repetition rate mode-locked Er-doped fiber laser using an allpolarization-maintaining (PM) nonlinear amplifying loop mirror. Stable single pulse generation had a pulse energy of 4 nJ and a repetition rate as low as 1.96 MHz. A section of standard single mode PM fiber was used to compress the output pulses from 2.5 ps down to 660 fs, being nearly transform-limited. The robust mode-locking design and all-PM cavity make this fiber laser stable and compact. The output average power fluctuation is within 0.17% over 10 h. Therefore, this source is ideally suitable for high pulse energy applications, such as micro-machining and nonlinear microscopy through further amplification.

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“…Over the last decade, femtosecond fiber lasers have also shown tremendous progress in nonlinear microscopy. [21][22][23] Much research activity has focused on the development of these robust and compact laser sources. They offer high average powers with high beam quality and have low fabrication costs with low demands on peak power, polarization, emission band, and pulse quality.…”

Section: Introductionmentioning

confidence: 99%

Epi-detecting label-free multimodal imaging platform using a compact diode-pumped femtosecond solid-state laser

Andreana

Le²,

Hansen

et al. 2017

J. Biomed. Opt

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-detecting label-free multimodal imaging platform using a compact diode-pumped femtosecond solid-state laser," J. Biomed. Opt. 22(9), 091517 (2017), doi: 10.1117/1.JBO.22.9.091517. Abstract. We have developed an epi-detected multimodal nonlinear optical microscopy platform based on a compact and cost-effective laser source featuring simultaneous acquisition of signals arising from hyperspectral coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence, and second harmonic generation. The laser source is based on an approach using a frequency-doubled distributed Bragg reflector-tapered diode laser to pump a femtosecond Ti:sapphire laser. The operational parameters of the laser source are set to the optimum trade-off between the spectral and temporal requirements for these three modalities, achieving sufficient spectral resolution for CARS in the lipid region. The experimental results on a biological tissue reveal that the combination of the epi-detection scheme and the use of a compact diode-pumped femtosecond solid-state laser in the nonlinear optical microscope is promising for biomedical applications in a clinical environment. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Multimodal fiber source for nonlinear microscopy based on a dissipative soliton laser

Cited by 18 publications

References 33 publications

High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser

High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser

Low-repetition-rate, high pulse energy all-polarization-maintaining Er-doped fiber laser mode-locked with a nonlinear amplifying loop mirror

Epi-detecting label-free multimodal imaging platform using a compact diode-pumped femtosecond solid-state laser

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