Multimodal mechanosensing enables treefrog embryos to escape egg-predators

Jung, Julie; Rojas, Shirley Jennifer Serrano; Warkentin, Karen M.

doi:10.1101/2020.09.18.304295

Cited by 4 publications

(1 citation statement)

References 104 publications

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“…Hair cells (inside the neuromasts) depolarize and release neurotransmitters to the afferent neuronal terminals after water-flow deflection. These terminals transmit this information to the posterior brain (Jung et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Can Carbon Nanofibers Affect Anurofauna? Study Involving NeotropicalPhysalaemus cuvieri(Fitzinger, 1826) Tadpoles

Guimarães

Estrela

Rodrigues

et al. 2021

Preprint

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Although carbon nanotubes (CNTs) toxicity in different experimental systems (in vivo and in vitro) is known, little is known about the toxic effects of carbon nanofibers (CNFs) on aquatic vertebrates. We herein investigated the potential impact of CNFs (1 and 10 mg/L) by using Physalaemus cuvieri tadpoles as experimental model. CNFs were able to induce nutritional deficit in animals after 48-h exposure to them, and this finding was inferred by reductions observed in body concentrations of total soluble carbohydrates, total proteins, and triglycerides. The increased production of hydrogen peroxide, reactive oxygen species and thiobarbituric acid reactive substances in tadpoles exposed to CNFs has suggested REDOX homeostasis change into oxidative stress. This process was correlated to the largest number of apoptotic and necrotic cells in the blood of these animals. On the other hand, the increased superoxide dismutase and catalase activity has suggested that the antioxidant system of animals exposed to CNFs was not enough to maintain REDOX balance. In addition, CNFs induced increase in acetylcholinesterase and butyrylcholinesterase activity, as well as changes in the number of neuromats evaluated on body surface (which is indicative of the neurotoxic effect of nanomaterials on the assessed model system). To the best of our knowledge, this is the first report on the impact of CNFs on amphibians; therefore, it broadened our understanding about ecotoxicological risks associated with their dispersion in freshwater ecosystems and possible contribution to the decline in the populations of anurofauna species.

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Section: Discussionmentioning

confidence: 99%

Can Carbon Nanofibers Affect Anurofauna? Study Involving NeotropicalPhysalaemus cuvieri(Fitzinger, 1826) Tadpoles

Guimarães

Estrela

Rodrigues

et al. 2021

Preprint

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A sensation for inflation: initial swim bladder inflation in larval zebrafish is mediated by the mechanosensory lateral line

Venuto

Thibodeau-Beganny

Trapani

et al. 2023

Preprint

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Larval zebrafish achieve neutral buoyancy between 4-5 days post-fertilization by swimming up to the surface and taking in air through their mouths to inflate their swim bladders. We define this behavior as 'surfacing'. Little is known about the sensory basis for this underappreciated behavior of larval fish. A strong candidate is the mechanosensory lateral line, a hair cell-based sensory system that detects hydrodynamic information from sources like water currents, predators, prey, and surface waves. However, a role for the lateral line in mediating initial inflation of the swim bladder has not been reported. To explore the connection between the lateral line and surfacing, we utilized a genetic mutant (lhfpl5b-/-) that renders the zebrafish lateral line insensitive to mechanical stimuli. We observe that approximately half of these lateral line mutants over-inflate their swim bladders during initial inflation and become positively buoyant. Thus, we hypothesize that larval zebrafish use their lateral line to moderate interactions with the air-water interface during surfacing to regulate swim bladder inflation. To test the hypothesis that lateral line defects are responsible for swim bladder over-inflation, we show exogenous air is required for the hyperinflation phenotype and transgenic rescue of hair cell function restores normal inflation. We also find that chemical ablation of anterior lateral line hair cells in wild type larvae causes hyperinflation. Furthermore, we show that manipulation of lateral line sensory information results in abnormal inflation. Finally, we report spatial and temporal differences in the surfacing behavior between wild type and lateral line mutant larvae. In summary, we propose a novel sensory basis for achieving neutral buoyancy where larval zebrafish use their lateral line to sense the air-water interface and regulate initial swim bladder inflation.

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The role of vibration amplitude in the escape hatching response of red-eyed treefrog embryos

Jung,

Caldwell,

McDaniel

et al. 2024

Preprint

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The function and adaptive significance of defensive behaviors depend on the contexts in which they naturally occur. Amplitude properties of predator cues are widely used by prey to assess predation risk, yet rarely studied in the context of the stimuli relevant to defensive decisions in nature. Red–eyed treefrog embryos,Agalychnis callidryas, hatch precociously in response to attacks on their arboreal egg clutches by snakes and wasps. They use vibrations excited during attacks to detect predators, but wind and rainstorms also excite intense vibrations. Past work has demonstrated that to avoid costly decision errors,A. callidryasnon–redundantly combine information from the temporal and frequency properties of clutch vibrations. Here we demonstrate that embryos also use absolute amplitude and fine–scale amplitude modulation information to refine their hatching decision. We used vibration recordings to characterize the amplitude properties of the most common predator and benign–source disturbances toA. callidryasegg clutches in nature and tested whether embryos at three ages across the onset of mechanosensory–cued hatching (4–6 days) respond to amplitude variation during playback of synthetic vibrations to eggs. Older embryos responded to much lower–amplitude vibrations, reflecting a >88–fold decrease in response threshold from 4 to 5 days. To assess how embryos combine amplitude with other vibration properties, we played embryos recorded exemplars of snake attack and rain vibrations of varying amplitudes and patterns of amplitude modulation. The amplitude response curve was steeper for snake recordings than for rain. While amplitude information alone is insufficient to discriminate predator attack from benign–source vibrations,A. callidryasemploy an impressively complex strategy combining absolute amplitude, amplitude modulation, temporal, and frequency information for their hatching decision.

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Multimodal mechanosensing enables treefrog embryos to escape egg-predators

Cited by 4 publications

References 104 publications

Can Carbon Nanofibers Affect Anurofauna? Study Involving NeotropicalPhysalaemus cuvieri(Fitzinger, 1826) Tadpoles

Can Carbon Nanofibers Affect Anurofauna? Study Involving NeotropicalPhysalaemus cuvieri(Fitzinger, 1826) Tadpoles

A sensation for inflation: initial swim bladder inflation in larval zebrafish is mediated by the mechanosensory lateral line

The role of vibration amplitude in the escape hatching response of red-eyed treefrog embryos

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