Multimodal study of CHI3L1 inhibition and its effect on angiogenesis, migration, immune response and refractive index of cellular structures in glioblastoma

Rusak, Agnieszka; Buzalewicz, Igor; Mrozowska, Monika; Wiatrak, Benita; Haczkiewicz-Leśniak, Katarzyna; Olbromski, Mateusz; Kmiecik, Alicja; Krzyżak, Edward; Pietrowska, Aleksandra; Moskal, Jakub; Podhorska‐Okołów, Marzenna; Podbielska, Halina; Dzięgiel, Piotr

doi:10.1016/j.biopha.2023.114520

Cited by 9 publications

(7 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…E-cadherin (mouse monoclonal, 1:1000, sc-8426, Santa Cruz), N-cadherin (mouse monoclonal, 1:1000, sc-8424, Santa Cruz), β-tubulin (rabbit polyclonal, 1:1000, ab6046, in 0.1% BSA in 0. 1% TBST, ab6046, Abcam, Cambridge, UK), VEGFA (mouse monoclonal, 1:1000, M7273, Dako, Glostrup, Denmark), VEGFC (mouse monoclonal, 101-M90, ReliaTech, Wolfenbüttel, Germany), VEGFD (mouse monoclonal, MAB286, R&D) [7].…”

Section: Western Blotmentioning

confidence: 99%

“…THP-1 cells were cultured for 24 hours in complete RPMI-1640 medium (Gibco), supplemented with 100 nM PMA (phorbol 12-myristate 13-acetate) (Sigma-Aldrich) [28], [7]. Subsequently, adherent macrophages were identi ed at the bottom of the vessel.…”

Section: Monocyte Differentiationmentioning

confidence: 99%

“…Of the chitinases found in human tissues, only mammalian acid chitinase and chitotriosidase exhibit chitinase activity. However, chitinaselike proteins, including CHI3L1, CHI3L2 and SI-CLP (stabilin-1 interacting chitinase-like protein), have biological functions that contribute to stimulation of cell proliferation, support of angiogenesis and tumor invasion, cell interactions and immunomodulation [1][2][3][4][5][6][7]. The best-known protein in this family is undoubtedly CHI3L1, also known as YKL-40.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

New insights into the role of the CHI3L2 protein in invasive ductal breast carcinoma: the CHI3L1 protein has a different role

Rusak,

Kątnik,

Górnicki

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Purpose Chitinase-like proteins have multiple biological functions that promote tumor growth, angiogenesis and metastasis. Expression of CHI3L2, which is similar in structure to CHI3L1, is detected in glioma cells and tumor-associated macrophages (TAMs) in glioma and breast cancer. However, its exact role remains unclear. Methods We analyzed the expression of CHI3L2 in 74 invasive ductal breast carcinoma (IDC) tumors, breast cancer and macrophages cell cultures using immunohistochemistry, immunofluorescence, Western blot and PCR methods. Clinicopathologic data were included in the analysis. Results The results obtained show that CHI3L2 expression decreases with increasing degree of tumor grade and negative status of estrogen (ER) and progesterone receptors (PR). Furthermore, CHI3L2 is significantly and positively correlated with phosphorylation of STAT-3 and ERK1/2 signaling pathways, but negatively correlated with macrophage infiltration. Furthermore, CHI3L2 is expressed both in the cytoplasm of cancer cells and in macrophages. Analysis of the clinicopathologic data revealed that CHI3L2 levels had no effect on patient survival. Discussion CHI3L2 expression may be specific for cancer cells in IDC and involved in cross-talk with the tumor microenvironment. Our study has shown that IDC cancer cells express the CHI3L2 protein, possibly indicating a novel function of this protein.

show abstract

Section: Western Blotmentioning

confidence: 99%

Section: Monocyte Differentiationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

New insights into the role of the CHI3L2 protein in invasive ductal breast carcinoma: the CHI3L1 protein has a different role

Rusak,

Kątnik,

Górnicki

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Antibody-based therapies also facilitate the immune-mediated clearance of CHI3L1 (YKL40)-expressing cancer cells. In vitro studies have demonstrated that CHI3L1 (YKL40) inhibition may have an influence on angiogenesis, migration, and immune response in various tumor cells [ 71 , 85 ]. However, the complexity, heterogeneity, and various molecular pathways all contribute to cancer development and the therapeutic response in the tumor microenvironment.…”

Section: Chi3l1 (Ykl40)—related Therapymentioning

confidence: 99%

The Role of Chitinase-3-like Protein-1 (YKL40) in the Therapy of Cancer and Other Chronic-Inflammation-Related Diseases

Chang,

Chen,

Chiang

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

Chitinase-3-like protein-1 (CHI3L1), also known as YKL40, is a glycoprotein that belongs to the chitinase protein family. It is involved in various biological functions, including cell proliferation and tissue remodeling, with inflammatory and immunomodulatory capabilities. Several studies have shown that CHI3L1(YKL40) is upregulated in various diseases, such as cancer, asthma, and inflammatory bowel disease, among others. Although the expression level of CHI3L1(YKL40) is associated with disease activity, severity, and prognosis, its potential as a therapeutic target is still under investigation. In this review, we summarize the biological functions, pathological roles, and potential clinical applications of specific inhibitors and targeted therapies related to CHI3L1(YKL40).

show abstract

“…The risk of HCC in autoimmune hepatitis is lower than that of other chronic liver diseases. A long-term follow-up study based on 1,428 patients with autoimmune hepatitis (AIH) found that only 1.7% of patients developed HCC with cirrhosis, only mildly increased the risk of HCC (80). Clinical and basic research on autoimmune liver disease in China has just begun, and there is a lack of relevant epidemiologic information, resulting in serious clinical underdiagnosis and mistreatment.…”

Section: Other Liver Diseasesmentioning

confidence: 99%

Research advances in serum chitinase-3-like protein 1 in liver fibrosis

Hu,

Liu,

Liu

et al. 2024

Front. Med.

View full text Add to dashboard Cite

While liver fibrosis remains a serious, progressive, chronic liver disease, and factors causing damage persist, liver fibrosis may develop into cirrhosis and liver cancer. However, short-term liver fibrosis is reversible. Therefore, an early diagnosis of liver fibrosis in the reversible transition phase is important for effective treatment of liver diseases. Chitinase-3-like protein 1 (CHI3L1), an inflammatory response factor that participates in various biological processes and is abundant in liver tissue, holds promise as a potential biomarker for liver diseases. Here, we aimed to review research developments regarding serum CHI3L1 in relation to the pathophysiology and diagnosis of liver fibrosis of various etiologies, providing a reference for the diagnosis, treatment, and prognosis of liver diseases.

show abstract

Multimodal study of CHI3L1 inhibition and its effect on angiogenesis, migration, immune response and refractive index of cellular structures in glioblastoma

Cited by 9 publications

References 80 publications

New insights into the role of the CHI3L2 protein in invasive ductal breast carcinoma: the CHI3L1 protein has a different role

New insights into the role of the CHI3L2 protein in invasive ductal breast carcinoma: the CHI3L1 protein has a different role

The Role of Chitinase-3-like Protein-1 (YKL40) in the Therapy of Cancer and Other Chronic-Inflammation-Related Diseases

Research advances in serum chitinase-3-like protein 1 in liver fibrosis

Contact Info

Product

Resources

About