Multimodal widefield fluorescence imaging with nonlinear optical microscopy workflow for noninvasive oral epithelial neoplasia detection: a preclinical study

Pal, Rahul; Villarreal, Paula; Yu, Xiaoying; Qiu, Suimin; Vargas, Gracie

doi:10.1117/1.jbo.25.11.116008

Cited by 8 publications

(3 citation statements)

References 73 publications

(110 reference statements)

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“…Two or more optical modalities can simultaneously probe different pathologic changes with better diagnostic performance than a single modality. Current efforts in development for oral multimodal in vivo imaging systems include fluorescence lifetime imaging, ultrasound backscatter microscopy, and photoacoustic imaging (Fatakdawala et al 2013); fluorescence lifetime imaging and RCM (Malik et al 2016); AFI and RCM (Quang et al 2017); macroscopic white light, AFI, and high-resolution microendoscopy (Yang et al 2019); and AFI and multiphoton microscopy (Pal et al 2020).…”

Section: Application Of Multimodal Optical Techniquesmentioning

confidence: 99%

Optical Imaging in the Diagnosis of OPMDs Malignant Transformation

Deng

Sun

et al. 2022

J Dent Res

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Oral potentially malignant disorders (OPMDs) are a heterogeneous group of oral lesions with a variable risk of malignant transformation to oral squamous cell carcinoma. The current OPMDs malignant transformation screening depends on conventional oral examination (COE) and is confirmed by biopsy and histologic examination. However, early malignant lesions with subtle mucosal changes are easily unnoticed by COE based on visual inspection and palpation. Optical techniques have been used to determine the biological structure, composition, and function of cells and tissues noninvasively by analyzing the changes in their optical properties. The oral epithelium and stroma undergo persistent structural, functional, and biochemical alterations during malignant transformation, leading to variations in optical tissue properties; optical techniques are thus powerful tools for detecting OPMDs malignant transformation. The optical imaging methods already used to detect OPMDs malignant transformation in vivo include autofluorescence imaging, narrowband imaging, confocal reflectance microscopy, and optical coherence tomography. They exhibit advantages over COE in detecting biochemical or morphologic changes at the molecular or cellular level in vivo; however, limitations also exist. This article comprehensively reviews the various real-time in vivo optical imaging methods used in the adjunctive diagnosis of OPMDs malignant transformation. We focus on the principles of these techniques, review their clinical application, and compare and summarize their advantages and disadvantages. Finally, we conclude with a discussion of current challenges and future directions of this field.

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Section: Application Of Multimodal Optical Techniquesmentioning

confidence: 99%

Optical Imaging in the Diagnosis of OPMDs Malignant Transformation

Deng

Sun

et al. 2022

J Dent Res

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“…11 The AF imaging can delineate areas containing high grade dysplasia and early oral cancer with improved sensitivity compared to clinical oral examination; however, specificity can be reduced due to loss of fluorescence associated with chronic inflammation. 12,13 To provide high sensitivity and specificity, multimodal imaging strategies combining widefield and high-resolution imaging have been developed with a variety of techniques, including nonlinear optical microscopy, 14 optical coherence tomography imaging with a forward-viewing probe, 15 reflectance confocal microscopy, 16 and fluorescence microscopy. [17][18][19][20] While promising, these approaches suffer a number of challenges, including ensuring accurate coregistration between widefield and microscopic images; providing image analysis results in real time; and ensuring that clinicians can translate results of multimodal image analysis displayed on a computer monitor to a precise location of where to perform a mucosal biopsy.…”

Section: Introductionmentioning

confidence: 99%

Multimodal optical imaging with real-time projection of cancer risk and biopsy guidance maps for early oral cancer diagnosis and treatment

et al. 2023

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. Significance Despite recent advances in multimodal optical imaging, oral imaging systems often do not provide real-time actionable guidance to the clinician who is making biopsy and treatment decisions. Aim We demonstrate a low-cost, portable active biopsy guidance system (ABGS) that uses multimodal optical imaging with deep learning to directly project cancer risk and biopsy guidance maps onto oral mucosa in real time. Approach Cancer risk maps are generated based on widefield autofluorescence images and projected onto the at-risk tissue using a digital light projector. Microendoscopy images are obtained from at-risk areas, and multimodal image data are used to calculate a biopsy guidance map, which is projected onto tissue. Results Representative patient examples highlight clinically actionable visualizations provided in real time during an imaging procedure. Results show multimodal imaging with cancer risk and biopsy guidance map projection offers a versatile, quantitative, and precise tool to guide biopsy site selection and improve early detection of oral cancers. Conclusions The ABGS provides direct visible guidance to identify early lesions and locate appropriate sites to biopsy within those lesions. This represents an opportunity to translate multimodal imaging into real-time clinically actionable visualizations to help improve patient outcomes.

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“…Multi-scale imaging strategies combining wide eld and high-resolution imaging have been developed; these strategies harness the high sensitivity of auto uorescence imaging to guide acquisition of highresolution images improving speci city and overcoming limitations associated with the small eld of view of high resolution imaging probes. Multiscale imaging approaches have been attempted using a variety of high resolution imaging techniques including nonlinear optical microscopy 30 , OCT imaging with a forward-viewing probe 31 , re ectance confocal microscopy 32 , and uorescence microscopy 33,34 .…”

mentioning

confidence: 99%

Multiscale Optical Imaging with Real Time Projection of Cancer Risk and Biopsy Guidance Maps for Early Oral Cancer Diagnosis and Treatment

Coole

Brenes

Mitbander

et al. 2022

Preprint

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When detected early, oral precancer and cancer can be treated with surgical resection with successful long-term clinical outcomes. Despite easy accessibility of the oral cavity, conventional oral examination has limited sensitivity and specificity to detect early neoplastic changes. Several optical imaging tools have shown promise to improve early detection of oral neoplasia, but they do not provide visible guidance to help clinicians determine when and where to biopsy suspicious oral lesions. Here, we demonstrate a low-cost, portable Active Biopsy Guidance System (ABGS) that uses multi-scale optical imaging with deep learning algorithms to create and directly project cancer risk maps onto clinically visible oral potentially malignant disorders (OPMDs) to guide biopsy locations in real time. Representative patient examples highlight these clinically actionable visualizations that are provided in real-time during an imaging procedure through automated processing of imaging data. Results show multiscale optical imaging with cancer-risk map projection offers a versatile, quantitative and precise tool to guide biopsy site selection and improve the early detection of oral epithelial cancers and their precursors.

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Multimodal widefield fluorescence imaging with nonlinear optical microscopy workflow for noninvasive oral epithelial neoplasia detection: a preclinical study

Cited by 8 publications

References 73 publications

Optical Imaging in the Diagnosis of OPMDs Malignant Transformation

Optical Imaging in the Diagnosis of OPMDs Malignant Transformation

Multimodal optical imaging with real-time projection of cancer risk and biopsy guidance maps for early oral cancer diagnosis and treatment

Multiscale Optical Imaging with Real Time Projection of Cancer Risk and Biopsy Guidance Maps for Early Oral Cancer Diagnosis and Treatment

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