Multimode fibre based imaging for optically cleared samples

Gusachenko, Ivan; Nylk, Jonathan; Tello, Javier A.; Dholakia, Kishan

doi:10.1364/boe.8.005179

Cited by 6 publications

(4 citation statements)

References 33 publications

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“…A sapphire fiber Raman imaging probe of diameter 60 micrometer, which can be used for endoscopic applications (in this case, a cladding will be required) and potentially for high temperature detections, is presented in this paper. This probe performs imaging without any mechanical motion and without any focusing optics at the tip, which makes it particularly interesting in harsh environments such as high temperature and/or in contact with corrosive liquids [29] and gases.…”

Section: Discussionmentioning

confidence: 99%

Raman imaging through multimode sapphire fiber

Deng¹,

Loterie²,

Konstantinou³

et al. 2019

Opt. Express

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We report on a sapphire fiber Raman imaging probe's use for challenging applications where access is severely restricted. Small-dimension Raman probes have been developed previously for various clinical applications because they show great capability for diagnosing disease states in bodily fluids, cells, and tissues. However, applications of these sub-millimeter diameter Raman probes were constrained by two factors: first, it is difficult to incorporate filters and focusing optics at such small scale; second, the weak Raman signal is often obscured by strong background noise from the fiber probe material, especially the most commonly used silica, which has a strong broad background noise in low wavenumbers (<500-1700 cm −1). Here, we demonstrate the thinnest-known imaging Raman probe with a 60 μm diameter Sapphire multimode fiber in which both excitation and signal collection pass through. This probe takes advantage of the low fluorescence and narrow Raman peaks of Sapphire, its inherent high temperature and corrosion resistance, and large numerical aperture (NA). Raman images of Polystyrene beads, carbon nanotubes, and CaSO 4 agglomerations are obtained with a spatial resolution of 1 μm and a field of view of 30 μm. Our imaging results show that single polystyrene bead (~15 µm diameter) can be differentiated from a mixture with CaSO 4 agglomerations, which has a close Raman shift.

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Section: Discussionmentioning

confidence: 99%

Raman imaging through multimode sapphire fiber

Deng¹,

Loterie²,

Konstantinou³

et al. 2019

Opt. Express

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“…In 2017, Gusachenko et al first employed MFSE for Raman spectroscopic imaging by replacing the photodetector in the common reflection imaging modality with the spectral measurement module as shown in Fig. 9(a) 142 . A continuous laser with 532 nm wavelength was focused by the MMF as the excitation beam to induce Raman scattering on the sample pixel-by-pixel.…”

Section: Raman Imagingmentioning

confidence: 99%

“…NA: numerical aperture of MMF; P: detector; S: light source; SK: CARS Stokes beam; PMT: Photomultiplier tube; FOUS: fiber-optic ultrasound sensor; R l and R a are the lateral and axial resolution, respectively; O: optical sectioning; A: acoustic sectioning; FWM: fourwave mixing.analysis of chemical compounds or biological substances by employing Raman imaging modalities, CARS imaging modalities, and second harmonic generation imaging modalities. For example, Gusachenko et al demonstrated using Raman imaging modalities of MFSE to identify the spatial distribution of different pharmaceuticals in samples142 . Cifuentes et al successfully employed the polarization-resolved second harmonic generation imaging modality of MFSE to characterize different structural proteins in the mouse tail tendon and heart tissue148 .…”

mentioning

confidence: 99%

Optical scanning endoscope via a single multimode optical fiber

Wu,

Zhu,

et al. 2024

OES

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Optical endoscopy has become an essential diagnostic and therapeutic approach in modern biomedicine for directly observing organs and tissues deep inside the human body, enabling non-invasive, rapid diagnosis and treatment. Optical fiber endoscopy is highly competitive among various endoscopic imaging techniques due to its high flexibility, compact structure, excellent resolution, and resistance to electromagnetic interference. Over the past decade, endoscopes based on a single multimode optical fiber (MMF) have attracted widespread research interest due to their potential to significantly reduce the footprint of optical fiber endoscopes and enhance imaging capabilities. In comparison with other imaging principles of MMF endoscopes, the scanning imaging method based on the wavefront shaping technique is highly developed and provides benefits including excellent imaging contrast, broad applicability to complex imaging scenarios, and good compatibility with various well-established scanning imaging modalities. In this review, various technical routes to achieve light focusing through MMF and procedures to conduct the scanning imaging of MMF endoscopes are introduced. The advancements in imaging performance enhancements, integrations of various imaging modalities with MMF scanning endoscopes, and applications are summarized. Challenges specific to this endoscopic imaging technology are analyzed, and potential remedies and avenues for future developments are discussed.

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“…This subject arises other interests and a myriad of applications among them micromanipulation [20], optical clearing [21] and optical tweezers [22]. Also multimode imaging brings direct applicabilities to the biological field, like endoscopes composed of a single MMF or either a bundle of them which offers more independent channels to the the imaging system [23,24].…”

Section: Wavefront Shapingmentioning

confidence: 99%

Focusing in multimode waveguides by wavefront shaping

Elias da Silva Pellegrini

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To begin with, I would like to express my warm gratitudes to prof. Lucas Gabrielli. Thank you for all the patience and for being there during all this work. I have learned a lot from you during these years.I am very privileged to come from such a special family who I deeply love. I would like to thank my parents for all the effort they made to invest in my education. My father has played a major role in my life by instigating my creativity and participating in every way he could, while my mother has always surrounded me with unconditional love and comfort. I would also like to thank my grandparents for taking such good care of our family. I feel very lucky to have, at home, my mother and my grandmother as examples of strong and powerful women. And, of course, I would like to thank my uncle for being so close to me. He was the first person to talk about Physics to me, he was the one who expanded my horizons and showed me how much we still have to know.I would like to give thanks to everybody from LEMAC, LCON and CTI for all the fruitful conversation. Especially, a heartfelt thank you to Paulo Jarshel, Julian Pita and Claudecir Biazoli for all the help they gave me during this work.These years would definitely be harder without my dear friends. The ones that made this journey so joyful and much more fun. I have also learned a lot from them. Thanks to my friends who were partners in so many trips, hikes and climbs, the ones who faced a pandemics with me and those that were always there, for the better and for the worse. Thanks for being with me everyday, for being my support in the difficult times and for all the good laughter that we have shared. You have made all the difference.Finally, I am very grateful for every teacher that I have ever had. I appreciate all the people that accounted for my education and all the ones that played part in the well functioning of the university.Also, I would like to acknowledge the financial aid received by CNPQ, with grant 168970/2018-0. ResumoEste trabalho tem como objetivo demonstrar como gerar foco com fibras ópticas multimodo através da modulação de fase das frentes de onda. Para realizar os experimentos de imageamento, em 633 nm, um modulador espacial de fase foi codificado para modular as frentes de onda do feixe incidente com máscaras de fase. Então, ao fazer a aquisição do padrão de espalhamento na saída da fibra óptica, uma relaçao entre fase e magnitude foi estabelecida e a matriz de transmissão das fibras foi calculada.A montagem experimental proposta, neste trabalho, é relativamente simples. Ela é mecanicamente estável e não requer nenhum feixe de referência, assim, utilizando as matrizes de transmissão calculadas, pontos focais podem ser gerados na saída da fibra.Este experimento de imageamento pode ser aplicado em diversas áreas, como bio imageamento e sistemas integrados. Para explorar essas possibilidades, experimento de acoplamento foram realizados. Acoplamentos fibra-fibra e fibra-chip foram estudados e mostramos que a potência de saída teve ganhos de até 4.5 db...

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Multimode fibre based imaging for optically cleared samples

Cited by 6 publications

References 33 publications

Raman imaging through multimode sapphire fiber

Raman imaging through multimode sapphire fiber

Optical scanning endoscope via a single multimode optical fiber

Focusing in multimode waveguides by wavefront shaping

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