Multinucleate cells (MNC) as sensitive semiquantitative biomarkers of the toxic effect after experimental fibrous dust and cigarette smoke inhalation by rats

Beňo, Milan; Hurbánková, Marta; Dušinská, Maria; Černá, Silvia; Volkovová, Katarína; Staruchova, Marta; Barančoková, M.; Kažimı́rová, Alena; Kovačíková, Zuzana; Mikulecký, M; Kyrtopoulos, Soterios A.

doi:10.1016/j.etp.2005.04.003

Cited by 13 publications

(11 citation statements)

References 30 publications

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“…MNC were increased after exposure (separate or in combination) to tobacco smoke as well as both fiber concentrations but significantly only after higher dose without smoking. Similarly, immature forms of AM in all exposed groups were increased in comparison with control (Beňo et al, 2005). Strongly dose dependent decrease of AM viability (higher dose with and without smoking) as well as phagocytic activity of AM (all group with smoking) was found in this experiment.…”

Section: Combined Effect Of Mineral Fibers and Tobacco Smoke On Respimentioning

confidence: 51%

“…In addition to biomarkers of immunotoxicity, biomarkers of genotoxicity (Beňo et al, 2005;Dusinska et al, 2004;Horská et al, 2006;Topinka et al, 2004;, oxidative damage and antioxidant defense (Staruchova et al, 2008) were also examined in the same cohorts. The studies involved workers with at least 5 years' exposure to asbestos, rock wool and glass fibers, respectively, at 3 industrial plants in Slovakia.…”

Section: Molecular Epidemiological Studies In Human Populationmentioning

confidence: 99%

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Immune System and Environmental Xenobiotics - The Effect of Selected Mineral Fibers and Particles on the Immune Response

Kuricová¹,

Tulinská²,

Líšková³

et al. 2012

Recent Advances in Immunology to Target Cancer, Inflammation and Infections

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Section: Combined Effect Of Mineral Fibers and Tobacco Smoke On Respimentioning

confidence: 51%

Section: Molecular Epidemiological Studies In Human Populationmentioning

confidence: 99%

Immune System and Environmental Xenobiotics - The Effect of Selected Mineral Fibers and Particles on the Immune Response

Kuricová¹,

Tulinská²,

Líšková³

et al. 2012

Recent Advances in Immunology to Target Cancer, Inflammation and Infections

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“…Their number may rise after long-term exposure to genotoxicants such as tobacco smoke, chemicals) (29-32) or certain dusts (33,34). BNC and multinucleate cell (MNC) counts in lung suspension may well refl ect chronic infl ammation, and may serve as good semi-quantitative biomarkers (35). In our study, BNC proportion in BALF increased to almost 2 % in the rats exposed to exometabolites and DAS.…”

Section: Infl Ammatory Activity Of S Chartarum Metabolites In Animalmentioning

confidence: 62%

Inflammatory and Haematotoxic Potential of Indoor Stachybotrys chartarum (Ehrenb.) Hughes Metabolites

Piecková

Hurbánková

Černá

et al. 2009

Archives of Industrial Hygiene and Toxicology

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Mould Stachybotrys chartarum (Ehrenb.) Hughes is known to pose a health risk in indoor environments. Most of its strains can produce several intra-and extracellular trichothecene mycotoxins. Complex secondary metabolites of stachybotrys isolates from mouldy dwellings/public buildings in Slovakia were intratracheally instilled in Wistar male rats (4 µg in 0.2 mL of 0.2 % dimethylsulphoxide; diacetoxyscirpenol as the positive control). After three days, haematological parameters were measured in peripheral blood and infl ammatory response biomarkers in bronchoalveolar lavage fl uid (BALF), and the results were statistically analysed. Exometabolites proved to suppress red blood cell (RBC), decreasing the total RBC count, haemoglobin, and haematocrit. The exposed rats showed signifi cantly higher total BALF cell count, indicating infl ammation, lower alveolar macrophage counts, and increased granulocyte count related to the BALF cells. Due to haematotoxic and infl ammation-inducing properties, metabolites of S. chartarum can cause damage to the airways and haematological disorders in occupants of mouldy buildings.

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“…Inhalation of inorganic particles is also known to induce MGC formation. MGC have been observed in the lungs of rodents after exposure to silica [115,116], asbestos [115,117], sepiolite nanoclay [116], silver nanowires [118], or multiwalled carbon nanotubes [119]. Giant cell interstitial pneumonitis is a pathological pattern of hard metal lung disease that is characterized by the presence of MGC [120,121].…”

Section: Interaction With Particulatesmentioning

confidence: 98%

Macrophage and Multinucleated Giant Cell Classification

Trout

Jessop

Migliaccio

2016

Current Topics in Environmental Health and Preventive Medicine

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The purpose of this chapter is to provide an overview of macrophage subtype and multinucleated giant cell classification with a specific discussion of their role(s) in response to particulates and other foreign bodies. Topics covered for the different subtypes include the following: environmental factors involved in their generation, functional characterization, disease associations, and interactions with particulates. This chapter is separated into three major parts. The first portion describes the normal structure and functions of the macrophage. Second, the currently published macrophage subsets are outlined. The classifications included in the discussion are based on function ("M" polarization) rather than anatomical position (tissue-specific macrophages -Kupffer cells, alveolar macrophages, etc.). As shown in Fig. 1.1, the ontogeny of the various types of macrophages being discussed in this chapter depends on the pathway of activation. The third major section focuses on multinucleated giant cells, which are formed by fusion of individual macrophages. The ontogeny of each subset will be discussed and the current literature regarding particulate/foreign-body interaction will be reviewed.

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Multinucleate cells (MNC) as sensitive semiquantitative biomarkers of the toxic effect after experimental fibrous dust and cigarette smoke inhalation by rats

Cited by 13 publications

References 30 publications

Immune System and Environmental Xenobiotics - The Effect of Selected Mineral Fibers and Particles on the Immune Response

Immune System and Environmental Xenobiotics - The Effect of Selected Mineral Fibers and Particles on the Immune Response

Inflammatory and Haematotoxic Potential of Indoor Stachybotrys chartarum (Ehrenb.) Hughes Metabolites

Macrophage and Multinucleated Giant Cell Classification

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