Multiomics in the central Arctic Ocean for benchmarking biodiversity change

Möck, Thomas; Boulton, William; Balmonte, John-Paul; Barry, Kevin; Bertilsson, Stefan; Bowman, Jeff S.; Buck, Moritz; Bratbak, Gunnar; Chamberlain, Emelia J.; Cunliffe, Michael; Creamean, Jessie M.; Ebenhöh, Oliver; Eggers, Sarah Lena; Fong, Allison A.; Gardner, Jessie; Gradinger, Rolf; Granskog, Mats A; Havermans, Charlotte; Hill, Thomas E.; Hoppe, Clara Jule Marie; Korte, Kerstin; Larsen, Aud; Müller, Oliver; Nicolaus, Anja; Oldenburg, Ellen; Popa, Ovidiu; Rogge, Swantje; Schäfer, Hendrik; Shoemaker, Katyanne; Snoeijs, Pauline; Torstensson, Anders; Valentin, Klaus; Vader, Anna; Barry, Kerrie; Chen, I.-M. A.; Clum, Alicia; Copeland, Alex; Daum, Chris; Eloe‐Fadrosh, Emiley A.; Foster, Brian; Foster, Bryce; Grigoriev, Igor V.; Ivanova, Natalia V.; Kuo, Alan; Kyrpides, Nikos; Mukherjee, Supratim; Palaniappan, Krishnaveni; Reddy, T. B. K.; Salamov, Asaf; Roux, Simon; Varghese, Neha; Woyke, Tanja; Wu, Dongying; Leggett, Richard M.; Moulton, Vincent; Metfies, Katja

doi:10.1371/journal.pbio.3001835

Cited by 9 publications

(5 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To corroborate the associations of signature populations with distinct environmental conditions, we assessed their spatiotemporal dynamics across the Arctic Ocean. This comparison included an additional 59 metagenomes as well as 1184 MAGs and metagenomic bins from the Fram Strait [ 22 ], the Tara Arctic expedition (TARA) [ 74 ], and the MOSAiC expedition [ 75 ]. Combined, these datasets provide an extensive geographical and seasonal coverage, from above the continental shelf in summer to the central basin in winter.…”

Section: Resultsmentioning

confidence: 99%

Atlantic water influx and sea-ice cover drive taxonomic and functional shifts in Arctic marine bacterial communities

et al. 2023

Self Cite

View full text Add to dashboard Cite

The Arctic Ocean is experiencing unprecedented changes because of climate warming, necessitating detailed analyses on the ecology and dynamics of biological communities to understand current and future ecosystem shifts. Here, we generated a four-year, high-resolution amplicon dataset along with one annual cycle of PacBio HiFi read metagenomes from the East Greenland Current (EGC), and combined this with datasets spanning different spatiotemporal scales (Tara Arctic and MOSAiC) to assess the impact of Atlantic water influx and sea-ice cover on bacterial communities in the Arctic Ocean. Densely ice-covered polar waters harboured a temporally stable, resident microbiome. Atlantic water influx and reduced sea-ice cover resulted in the dominance of seasonally fluctuating populations, resembling a process of “replacement” through advection, mixing and environmental sorting. We identified bacterial signature populations of distinct environmental regimes, including polar night and high-ice cover, and assessed their ecological roles. Dynamics of signature populations were consistent across the wider Arctic; e.g. those associated with dense ice cover and winter in the EGC were abundant in the central Arctic Ocean in winter. Population- and community-level analyses revealed metabolic distinctions between bacteria affiliated with Arctic and Atlantic conditions; the former with increased potential to use bacterial- and terrestrial-derived substrates or inorganic compounds. Our evidence on bacterial dynamics over spatiotemporal scales provides novel insights into Arctic ecology and indicates a progressing Biological Atlantification of the warming Arctic Ocean, with consequences for food webs and biogeochemical cycles.

show abstract

Section: Resultsmentioning

confidence: 99%

Atlantic water influx and sea-ice cover drive taxonomic and functional shifts in Arctic marine bacterial communities

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…This includes regions, which are completely unexplored such as the Central Arctic Ocean and large parts of the Southern Ocean (not covered by the Tara Oceans Polar Circle expedition [15]) but also long-term monitoring could be improved. Recent expeditions such as the MOSAiC expedition between September 2019 and October 2020 as well as Synaptic Arctic Survey 2021 conducted sampling for (viral) metagenomics in the Central Arctic Ocean up to the North Pole [255,256] and will hopefully extend our knowledge about phages from these remote regions in the near future.…”

Section: Future Perspectivesmentioning

confidence: 99%

Breaking the Ice: A Review of Phages in Polar Ecosystems

Heinrichs,

Piedade,

Popa

et al. 2023

Methods in Molecular Biology

Self Cite

View full text Add to dashboard Cite

Bacteriophages, or phages, are viruses that infect and replicate within bacterial hosts, playing a significant role in regulating microbial populations and ecosystem dynamics. However, phages from extreme environments such as polar regions remain relatively understudied due to challenges like restricted ecosystem access and low biomass. Understanding the diversity, structure, and functions of polar phages is crucial for advancing our knowledge of the microbial ecology and biogeochemistry of these environments. In this review, we will explore the current state of knowledge on phages from the Arctic and Antarctic, focusing on insights gained from -omic studies, phage isolation, and virus-like particle abundance data. Metagenomic studies of polar environments have revealed a high diversity of phages with unique genetic characteristics, providing insights into their evolutionary and ecological roles. Phage isolation studies have identified novel phage-host interactions and contributed to the discovery of new phage species.Virus-like particle abundance and lysis rate data, on the other hand, have highlighted the importance of phages in regulating bacterial populations and nutrient cycling in polar environments. Overall, this review aims to provide a comprehensive overview of the current state of knowledge about polar phages, and by synthesizing these different sources of information, we can better understand the diversity, dynamics, and functions of polar phages in the context of ongoing climate change, which will help to predict how polar ecosystems and residing phages may respond to future environmental perturbations.

show abstract

“…Here, we used metagenome-informed genomics to explore the genetic diversity and predicted structural diversity of prokaryotic DUF3494 IBPs from the central Arctic Ocean. During leg 2 of the MOSAiC expedition, 15 metagenomic samples were collected spanning the bathypelagic, mesopelagic, epipelagic, sea-ice interface and interior ice layers [30]. Unlike in many other studies, these samples were collected during polar winter.…”

Section: Introductionmentioning

confidence: 99%

Genetic and Structural Diversity of Prokaryotic Ice-Binding Proteins from the Central Arctic Ocean

Winder

Boulton

Salamov

et al. 2023

Genes

Self Cite

View full text Add to dashboard Cite

Ice-binding proteins (IBPs) are a group of ecologically and biotechnologically relevant enzymes produced by psychrophilic organisms. Although putative IBPs containing the domain of unknown function (DUF) 3494 have been identified in many taxa of polar microbes, our knowledge of their genetic and structural diversity in natural microbial communities is limited. Here, we used samples from sea ice and sea water collected in the central Arctic Ocean as part of the MOSAiC expedition for metagenome sequencing and the subsequent analyses of metagenome-assembled genomes (MAGs). By linking structurally diverse IBPs to particular environments and potential functions, we reveal that IBP sequences are enriched in interior ice, have diverse genomic contexts and cluster taxonomically. Their diverse protein structures may be a consequence of domain shuffling, leading to variable combinations of protein domains in IBPs and probably reflecting the functional versatility required to thrive in the extreme and variable environment of the central Arctic Ocean.

show abstract

Multiomics in the central Arctic Ocean for benchmarking biodiversity change

Cited by 9 publications

References 11 publications

Atlantic water influx and sea-ice cover drive taxonomic and functional shifts in Arctic marine bacterial communities

Atlantic water influx and sea-ice cover drive taxonomic and functional shifts in Arctic marine bacterial communities

Breaking the Ice: A Review of Phages in Polar Ecosystems

Genetic and Structural Diversity of Prokaryotic Ice-Binding Proteins from the Central Arctic Ocean

Contact Info

Product

Resources

About