1994
DOI: 10.1016/0022-1759(94)90351-4
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Multiparametric analyses of hybridoma growth on glass cylinders in a packed-bed bioreactor system with internal aeration. Serum-supplemented and serum-free media comparison for MAb production

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Cited by 18 publications
(10 citation statements)
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“…The packed-bed system described here can last for months with the possibility of decreasing supplemented serum concentration and even using serum-free media [11]. In the present work we maintained serum at 10% for two reasons, one being not to introduce another variable and the second the dependence of this cell line on serum.…”
Section: Discussionmentioning
confidence: 99%
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“…The packed-bed system described here can last for months with the possibility of decreasing supplemented serum concentration and even using serum-free media [11]. In the present work we maintained serum at 10% for two reasons, one being not to introduce another variable and the second the dependence of this cell line on serum.…”
Section: Discussionmentioning
confidence: 99%
“…Glucose concentration was determined using glucose-oxidase method (Merck-Clevenot, France), lactate concentration through lactate dehydrogenase (Sentinel CH, Italy) and monoclonal antibody (MAb) concentration measured by an indirect method of the enzyme-linked immunosorbent assay (ELISA) [11], using the same monoclonal antibody puri®ed and quanti®ed by Lowry as the standard.…”
Section: Methodsmentioning
confidence: 99%
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“…Other techniques include growth of cells in dialysis tubing within a culture bottle (Sjbrgren-Jansson and Jeanson, 1990), a roller bottle-like apparatus (Falkenberg et al, 1993), or tumbling chamber (Jaspert et al, 1995); and the use of oscillating bubble dialysis chambers (Pannell and Milstein, 1992). Laboratory scale stirred tank reactors (Reuveny et al, 1986a;Shevitz et al, 1989), fermentors (Reuveny et al, 1986b;Falkenberg et al, 1995), ceramic-matrix bioreactors (Kurkela et al, 1993), packedbed bioreactors (Moro et al, 1994), and hollow fiber bioreactors (Hopkinson, 1985;Altshuler et al, 1986;Kurkela et al, 1993;Jackson et al, in press) are also in use.…”
Section: Alternativesmentioning
confidence: 99%