Multiparametric analysis of diversity in Botrytis cinerea isolates from Israel

Srivastava, Dhruv Aditya; Yakubov, Mariana; Feldbaum, Reut Amar; Tish, Nimrod; Shoyhet, Hagit; Manasherova, Ekaterina; Pandaranayaka, Eswari P. J.; Rav‐David, Dalia; Elad, Y.; Harel, Amnon

doi:10.1007/s12600-018-0684-4

Cited by 6 publications

(3 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These finding is in full accordance with the study on NB in Sardinia [24]. Ptm had also significant (20%) higher normalized virulence (measured by necrotrophic divided by saprotrophic growth rate) suggesting that the difference in virulence is not only related to the higher saprotrophic growth rate, and could result from additional virulence mechanisms (e.g., fungal effectors; [15]). The contribution of host defense mechanisms to this result is probably minor, considering that higher virulence of Ptm was observed on all four tested host lines (Figure 3).…”

Section: The Prominent Role Of P Teres F Maculatasupporting

confidence: 90%

See 1 more Smart Citation

Characterization of the Barley Net Blotch Pathosystem at the Center of Origin of Host and Pathogen

et al. 2019

Self Cite

View full text Add to dashboard Cite

Net blotch (NB) is a major disease of barley caused by the fungus Pyrenophora teres f. teres (Ptt), and P. teres f. maculata (Ptm). Ptt and Ptm infect the cultivated crop (Hordeum vulgare) and its wild relatives (H. vulgare ssp. spontaneum and H. murinum ssp. glaucum). The main goal of this research was to study the NB-causing pathogen in the crop center of origin. To address this, we have constructed a Ptt (n = 15) and Ptm (n = 12) collection isolated from three barley species across Israel. Isolates were characterized genetically and phenotypically. Aggressiveness of the isolates was determined based on necrotrophic growth rate on detached leaves of barley. In addition, isolates were genetically characterized by the mating type, followed by phylogenetic analysis, clustering them into seven groups. The analysis showed no significant differentiation of isolates based on either geographic origin, host of origin or form (Ptt vs. Ptm). Nevertheless, there was a significant difference in aggressiveness among the isolates regardless of host species, geographic location or sampling site. Moreover, it was apparent that the isolates derived from wild hosts were more variable in their necrotrophic growth rate, compared to isolates sampled from cultivated hosts, thereby suggesting that NB plays a major role in epidemiology at the center of barley origin where most of the diversity lies. Ptm has significantly higher necrotrophic and saprotrophic growth rates than Ptt, and for both a significant negative correlation was found between light intensity exposure and growth rates.

show abstract

Section: The Prominent Role Of P Teres F Maculatasupporting

confidence: 90%

“…Variability in saprotrophic growth rate was previously associated with the growth rate on leaf tissue [15]. We therefore measured necrotrophic growth rate on barley leaves by inoculating each isolate on four barley varieties ('Noga', 'Ma'anit', 'Sagiv', and 'Barke').…”

Section: Necrotrophic Growth Rates Reveal Substantial Variability Amomentioning

confidence: 99%

Characterization of the Barley Net Blotch Pathosystem at the Center of Origin of Host and Pathogen

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Considerable postharvest losses, due to B. cinerea have been found in: blackberry, blueberry, currant, grape, kiwi, pomegranate, quince, raspberries, strawberry, grapes and many other crops [ 2 , 3 ]. Capable of infecting over 580 genera of plants (including agriculturally important crops) [ 2 , 4 , 5 ], B. cinerea causes necrotic lesions in foliage and other plant parts, ultimately leading to plant death [ 6 , 7 ]. Taken together with the importance of this pathogen, and availability of molecular tools [ 8 ] and the genome [ 9 ], supporting functional analysis, it has become an important model for molecular studies of necrotrophic fungi.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of Botrytis cinerea

et al. 2020

Self Cite

View full text Add to dashboard Cite

The necrotrophic fungus Botrytis cinerea, is considered a major cause of postharvest losses in a wide range of crops. The common fungal extracellular membrane protein (CFEM), containing a conserved eight-cysteine pattern, was found exclusively in fungi. Previous studies in phytopathogenic fungi have demonstrated the role of membrane-bound and secreted CFEM-containing proteins in different aspects of fungal virulence. However, non-G protein-coupled receptor (non-GPCR) membrane CFEM proteins have not been studied yet in phytopathogenic fungi. In the present study, we have identified a non-GPCR membrane-bound CFEM-containing protein, Bcin07g03260, in the B. cinerea genome, and generated deletion mutants, ΔCFEM-Bcin07g03260, to study its potential role in physiology and virulence. Three independent ΔCFEM-Bcin07g03260 mutants showed significantly reduced progression of a necrotic lesion on tomato (Solanum lycopersicum) leaves. Further analysis of the mutants revealed significant reduction (approximately 20–30%) in conidial germination and consequent germ tube elongation compared with the WT. Our data complements a previous study of secreted ΔCFEM1 mutants of B. cinerea that showed reduced progression of necrotic lesions on leaves, without effect on germination. Considering various functions identified for CFEM proteins in fungal virulence, our work illustrates a potential new role for a non-GPCR membrane CFEM in pathogenic fungi to control virulence in the fungus B. cinerea.

show abstract

First report of Colletotrichum aenigma and C. perseae causing anthracnose disease on Capsicum annuum in Israel

et al. 2022

View full text Add to dashboard Cite

Multiparametric analysis of diversity in Botrytis cinerea isolates from Israel

Cited by 6 publications

References 44 publications

Characterization of the Barley Net Blotch Pathosystem at the Center of Origin of Host and Pathogen

Characterization of the Barley Net Blotch Pathosystem at the Center of Origin of Host and Pathogen

Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of Botrytis cinerea

First report of Colletotrichum aenigma and C. perseae causing anthracnose disease on Capsicum annuum in Israel

Contact Info

Product

Resources

About