Multiparametric comparison of mesenchymal stromal cells obtained from trabecular bone by using a novel isolation method with those obtained by iliac crest aspiration from the same subjects

Abstract

Trabecular bone fragments from femoral heads are sometimes used as bone grafts and have been described as a source of mesenchymal progenitor cells. Nevertheless, mesenchymal stromal cells (MSC) from trabecular bone have not been directly compared with MSC obtained under standard conditions from iliac crest aspiration of the same patients. This is the ideal control to avoid inter-individual variation. We have obtained MSC by a novel method (grinding bone fragments with a bone mill without enzymatic digestion) from the femoral heads of 11 patients undergoing hip replacement surgery and compared them with MSC obtained by standard iliac crest aspiration of bone marrow from the same patients. We have shown that trabecular bone MSC obtained by mechanically fragmented femoral heads fulfil the immunophenotypic and multilineage (adipogenic, osteogenic and chondrogenic) differentiation criteria used to define MSC. We have also differentially compared cellular yields, growth kinetics, cell cycle assessment, and colony-forming unit-fibroblast content of MSC from both sources and conclude that these parameters do not significantly differ. Nevertheless, the finding of slight differences, such as a higher expression of the immature marker CD90, a lower expansion time through the different passages, and a higher percentage of cycling cells in the trabecular bone MSC, warrants further studies with the isolation method proposed here in order to gain further knowledge of the status of MSC in this setting.

Isolation of equine multipotent mesenchymal stromal cells by enzymatic tissue digestion or explant technique: comparison of cellular properties

“…Therefore, we considered a non-enzymatic isolation technique to recover MSCs, which would potentially be less affected and damaged than by an enzymatic isolation technique [28,52,53]. In this study, the isolation of MSCs by explant technique was performed and compared to collagenase digestion.…”

Mouse Mandible Contains Distinctive Mesenchymal Stem Cells

“…Therefore, we employed an enzyme digestion method to isolate MSCs from mandibular bones, and verified their MSC properties by clonogenic, multilineage differentiation, and tissue regeneration assays in comparison to mouse BMMSCs. It was reported that bone and bone marrow contain MSCs with similar stem cell properties, but slightly different in terms of surface molecule expression (Sanchez-Guijo et al, 2009). It is likely that OMSCs contain post-migrating cranial neural crest cells (Chunk et al, 2009).…”

Titanium and tantalum as mesenchymal stem cell scaffolds for spinal fusion: an in vitro comparative study

“…Median age was 48 years (range 34-59 years) and male/female ratio was 4/1. MSC from BM were isolated as previously described [6,19]. Briefly, low-density mononuclear cells were isolated by Ficoll-Paque (Biochrom KG, Berlin, Germany) and plated at a cell density of 10 6 MNC/cm 2 on a plastic surface in DMEM (Gibco BRL, Paisley, UK) culture medium containing 10% of pre-selected fetal calf serum (FCS; BioWhittaker, Verviers, Belgium).…”

“…In order to demonstrate the multilineage differentiation potential of MSC in each experimental group, adipogenic, osteogenic and chondrogenic differentiation was induced as previously described [19].…”