Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy

Dalgarno, Paul A.; Dalgarno, H. I. C.; Putoud, A.; Lambert, Robert; Paterson, Lynn; Logan, David C.; Towers, David P.; Warburton, Richard J.; Greenaway, A. H.

doi:10.1364/oe.18.000877

Cited by 101 publications

(89 citation statements)

References 21 publications

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“…Evaluating both the defocused and focused images, they were able to follow 200 nm beads with a standard deviation of r \ 4 nm for a z-range of 600 nm. Another method to detect particle depth positions is the use of diffraction gratings Dalgarno et al 2010). Evaluating the defocused particle images for different orders of diffraction, it is possible to determine the depth position.…”

Section: Techniques Based On Out-of-focus Imaging Without Aperturementioning

confidence: 99%

Particle imaging techniques for volumetric three-component (3D3C) velocity measurements in microfluidics

Cierpka

Kähler

2011

J Vis

131

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The reliable measurement of the 3D3C velocity field in microfluidic devices becomes more and more important for future optimization and developments for lab-on-a-chip applications or point-of-care medical diagnosis systems. In the past years, different particle-based imaging methods, such as confocal scanning microscopy, holography, stereoscopic and tomographic imaging or approaches based on defocused particle images or optical aberrations have been developed and applied successfully to measure velocity fields in microfluidic systems. The benefits and drawbacks of these methods will be discussed in detail as the proper understanding of the measurement principle is essential to select the most appropriate technique for a desired measurement application. Once an imaging method is chosen, the velocity can be estimated by correlation-based methods or tracking approaches. The advantages and disadvantages of both methods and the importance of image preprocessing will also be discussed in detail.

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Section: Techniques Based On Out-of-focus Imaging Without Aperturementioning

confidence: 99%

Particle imaging techniques for volumetric three-component (3D3C) velocity measurements in microfluidics

Cierpka

Kähler

2011

J Vis

131

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“…A simpler version based on a grating that is able to image 3 planes simultaneously was presented before [3]. Alternatively, by inserting a microlens array into the detection path of a conventional microscope, one can capture light fields of biological specimens in a single exposure, from which images of individual planes can be recovered [4].…”

Section: Introductionmentioning

confidence: 99%

Rapid 3D light-sheet microscopy with a tunable lens

et al. 2013

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The in-vivo investigation of highly dynamic biological samples, for example the beating zebrafish heart, requires high-speed volume imaging techniques. Light-sheet microscopy is ideal for such samples as it records high-contrast images of entire planes within large samples at once. However, in order to obtain images of different planes, it has been necessary to move the sample relative to the fixed focal plane of the detection objective lens. This mechanical movement limits speed, precision and may be harmful to the sample. We have built a light-sheet microscope that uses remote focusing with an electrically tunable lens (ETL). Without moving specimen or objective we have thereby achieved flexible volume imaging at much higher speeds than previously reported. Our high-speed microscope delivers 3D snapshots of sensitive biological samples. As an example, we imaged 17 planes within a beating zebrafish heart at 510 frames per second, equivalent to 30 volume scans per second. Movements, shape changes and signals across the entire volume can be followed which has been impossible with existing reconstruction techniques. Abstract: The in-vivo investigation of highly dynamic biological samples, for example the beating zebrafish heart, requires high-speed volume imaging techniques. Light-sheet microscopy is ideal for such samples as it records high-contrast images of entire planes within large samples at once. However, in order to obtain images of different planes, it has been necessary to move the sample relative to the fixed focal plane of the detection objective lens. This mechanical movement limits speed, precision and may be harmful to the sample. We have built a light-sheet microscope that uses remote focusing with an electrically tunable lens (ETL). Without moving specimen or objective we have thereby achieved flexible volume imaging at much higher speeds than previously reported. Our high-speed microscope delivers 3D snapshots of sensitive biological samples. As an example, we imaged 17 planes within a beating zebrafish heart at 510 frames per second, equivalent to 30 volume scans per second. Movements, shape changes and signals across the entire volume can be followed which has been impossible with existing reconstruction techniques.

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“…Several techniques followed and were based on the simultaneous imaging of two or more planes in the sample. In a first implementation, several parallel planes are recorded simultaneously and combined to achieve volumetric imaging [7][8][9]. Currently this approach allows imaging of a volume of a few micrometers deep.…”

Section: Introductionmentioning

confidence: 99%

High-resolution real-time dual-view imaging with multiple point of view microscopy

Mangeol¹

2016

Biomed. Opt. Express

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Most methods to observe three-dimensional processes in living samples are based on imaging a single plane that is sequentially scanned through the sample. Sequential scanning is inherently slow, which can make it difficult to capture objects moving quickly in three dimensions. Here we present a novel method, multiple point-of-view microscopy (MPoVM), that allows simultaneous capturing of the front and side views of a sample with high resolution. MPoVM can be implemented in most fluorescence microscopes, offering new opportunities in the study of dynamic biological processes in three dimensions. 2. R. Tomer, K. Khairy, F. Amat, and P. J. Keller, "Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy," Nat. Methods 9(7), 755-763 (2012). 3. U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, "Multiview light-sheet microscope for rapid in toto imaging," Nat. Methods 9(7), 730-733 (2012) imaging using single-objective SPIM," Nat. Methods 12(7), 641-644 (2015). 34. S.-I. Chang and J.-B. Yoon, "Shape-controlled, high fill-factor microlens arrays fabricated by a 3D diffuser lithography and plastic replication method," Opt. Express 12(25), 6366-6371 (2004).

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Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy

Cited by 101 publications

References 21 publications

Particle imaging techniques for volumetric three-component (3D3C) velocity measurements in microfluidics

Particle imaging techniques for volumetric three-component (3D3C) velocity measurements in microfluidics

Rapid 3D light-sheet microscopy with a tunable lens

High-resolution real-time dual-view imaging with multiple point of view microscopy

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