Múltiplas estirpes de isolados de Mycobacteriumbovis identificados por tipagem molecular em bovinos abatidos em matadouros-frigoríficos

Abstract

Pesq. Vet. Bras. 34(2):103-108, fevereiro 2014 103 RESUMO.-O objetivo do presente trabalho foi utilizar mé-todos bacteriológicos e moleculares para a identificação do Mycobacterium bovis em lesões observadas em carcaças de bovinos durante a inspeção post mortem de rotina em matadouros-frigoríficos com serviço de inspeção oficial. Foi acompanhado o abate e a inspeção de 825.394 bovinos, sadios ao exame ante mortem pelo serviço de inspeção oficial em dez matadouros-frigoríficos do estado da Bahia. Carcaça de 180 bovinos apresentaram lesões sugestivas de tuberculose e por outras linfadenites. No isolamento bacteriano, 25 amostras apresentaram crescimento disgônico de colônias de coloração creme-amareladas em meio de cultura Stonebrink-Leslie. Desses isolados, 14 foram identifi- Múltiplas estirpes de isolados de Mycobacterium bovisidentificados por tipagem molecular em bovinos abatidos em matadouros-frigoríficos The aim of this study was to use bacteriological and molecular methods to identify Mycobacterium bovis in lesions observed in cattle carcasses during routine post-mortem inspection in slaughterhouses with official inspection service. It was accompanied the slaughter and inspection of 825,394 cattle, healthy ante mortem examination by the official inspection service in ten slaughterhouses in the state of Bahia. Carcasses of 180 cattle presented lesions suggestive of tuberculosis and other lymphadenitis. In bacterial isolation, 25 samples showed dysgonic growth of colonies of creamy-yellow in medium-Stonebrink Leslie. From these isolates, 14 were identified as M. bovis and the multiplex PCR technique spoligotyping was discriminated against eight different spoligotypes of M. bovis, seven previously described in the literature and a new spoligotypes without former description. The major spoligotypes was SB0121, with five samples which has been described in Brazil and other countries, followed by two clusters, SB295 and SB1055, with two isolates each. The SB1145 and SB1648 spoligotypes were reported only in Brazil and Denmark, respectively. The spoligotypes SB140 has been found in Brazil, Argentina, Uruguay and Paraguay. These results demonstrate that the spoligotypes obtained are shared, so far, among Brazilian states and among Latin America and Europe. Thus, molecular discrimination of isolates of M. bovis by Spoligotyping constitutes a tool for epidemiological studies of bovine tuberculosis in the state of Bahia. INDEX TERMS: Mycobacterium bovis, mycobacteria, molecular epidemiology, spoligotyping, tuberculosis, zoonosis, cattle. (Brasil 2006, Heinemann et al. 2008.A tuberculose no homem é causada principalmente pelo Mycobacterium tuberculosis, mas a doença causada por M. bovis tem sido reportada e representa um risco para a saúde pública (Parreiras et al. 2012, Reyes et al. 2012. Em 2011, 8,7 milhões de pessoas adoeceram e 1,4 milhões de óbitos anuais foi determinado pela tuberculose. A maioria dos óbitos ocorre em países de baixa e média renda e está entre as três principais causas de mortes em mulheres ent...

Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

“…Bovine tuberculosis predominantly affects cattle and buffaloes, but may occasionally infect other mammalian species, including humans (Etchechoury et al, 2010;Silva et al, 2018). It may spread through direct contact with infected animals, causing the spread of disease among herds or herds to wild animals and vice versa (Corner, Murphy, & Gormley, 2011;Reis, 2015), or being transmitted through indirect contact with contaminated equipment, water and food (Palmer, 2013;Smith, Tauer, Schukken, Lu, & Grohn, 2013).…”

Genomic and temporal analyses of Mycobacterium bovis in southern Brazil

“…Recently, this spoligotype was reported in northern Brazil [30], and had already been described in the states of Paraíba [83], Mato Grosso do Sul, Santa Catarina [84, 85], São Paulo [86], Bahia [87], Mato Grosso and Goiás [88]. SB0121 was the second most predominant spoligotype, and was also prevalent in previous studies in states of different Brazilian regions, including RS in southern Brazil [89]; São Paulo and Minas Gerais in south-eastern Brazil [84, 86]; Mato Grosso do Sul, Mato Grosso and Goiás in the midwest [84, 88]; and Bahia and Paraíba in the north-east [84, 87].…”

Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis

“…This result is similar to those described by Ramos et al (14), who reported a higher prevalence of lesions compatible with tuberculosis in tracheobronchial and mediastinal lymph nodes and lungs; such typical predominance of lesions in the respiratory tract is indicative for airborne transmission. On the other hand, Alzamora Filho et al (15) identified the most evident lesions in the lymph nodes of the head (retropharyngeal and parotid) with pulmonary parenchyma. These results corroborate with the findings of the present study, due to the typical predominance of lesions in the respiratory tract, suggesting the airway, as the main gateway for M. bovis in bovines.…”