Multiple amino acid substitutions between murine gamma 2a heavy chain Fc regions of Ig1a and Ig1b allotypic forms.

Dognin, Mai J.; Lauwereys, Marc; Strosberg, A. Donny

doi:10.1073/pnas.78.7.4031

Cited by 11 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This suggests that the antigenic determinants recognized by these RF are located in the hinge region or the CH3 domain of the IgG2a heavy chain, where most amino acid substitutions between the a and b allotypic forms of mouse IgG2a have been found (16,17). No such allotypic specificities have so far been detected with anti-IgG1 and anti-IgG2b clones.…”

Section: Discussionmentioning

confidence: 91%

Isotypic and allotypic specificity of mouse rheumatoid factors.

Vansnick¹,

Stassin²,

Lestre³

1983

The Journal of Experimental Medicine

View full text Add to dashboard Cite

The specificity of polyclonal mouse rheumatoid factors (RF) was analyzed by competition experiments with heat-aggregated mouse IgG subclasses. The RF spontaneously produced by three normal mouse strains (129/Sv, CBA/Ht, and C57Bl/6) and by two strains with autoimmune diseases (MRL/l and NZB) were found to consist of distinct non-cross-reactive antibody subpopulations each specific for one IgG subclass. The sera of the normal strains contained IgG1- and IgG2a-specific RF. The autoimmune strains produced an additional variety of RF that was specific for The autoimmune strains produced an additional variety of RF that was specific for IgG2b. Also, the RF secreted by spleen cells of various normal strains after in vitro polyclonal activation with lipopolysaccharide could be resolved into distinct subpopulations specific for IgG1 or IgG2a. These results were confirmed by the analysis of monoclonal RF derived from BALB/c, C57Bl/6, CBA/Ht, and 129/Sv mice: of 73 hybridomas with RF activity, 71 displayed a strict subclass specificity. The subclass predominantly recognized depended on the origin of the spleen cells used to generate the hybridomas. After polyclonal activation in vitro, a broad spectrum of different specificities was obtained with 16 RF specific for IgG1, 13 for IgG2a, and 4 for IgG2b. In contrast, 27 of 28 monoclonal RF derived from 129/Sv and BALB/c mice without prior polyclonal activation were specific for IgG2a, and of these 75% were allotype specific since they failed to react with IgG2a of the b allotype. These results demonstrate the importance of subclass specificity in the production of RF in vivo. With the exception of the IgG2b-specific clones, all these monoclonal RF reacted preferentially with heat-aggregated or antigen-bound IgG. Among the hybridomas generated by the fusion of in vitro polyclonally activated spleen cells of 4-wk-old mice, the frequency of clones with RF activity was at least 40 times higher than that of clones specific for mouse IgM, human IgG, ovalbumin, and hen lysozyme.

show abstract

Section: Discussionmentioning

confidence: 91%

Isotypic and allotypic specificity of mouse rheumatoid factors.

Vansnick¹,

Stassin²,

Lestre³

1983

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…Surprisingly, about 10% of the nucleotide positions in the sequences of these allelic genes differed. The predicted y2ab amino acid sequence, however, was almost identical to the amino acid sequence for the y2ab protein determined independently (6).…”

Section: Figmentioning

confidence: 88%

“…3. The deduced sequence ofthe S43 protein differs at only two positions (387 and 449) from the CH2 and CH3 sequences of the IgG2ab protein from CBPC 101 cells (6). Assuming no sequencing errors, the small differences could be explained by somatic mutations in the hybridoma or myeloma lines or possibly genetic polymorphisms.…”

Section: Figmentioning

confidence: 99%

“…Assuming no sequencing errors, the small differences could be explained by somatic mutations in the hybridoma or myeloma lines or possibly genetic polymorphisms. The homology between the deduced sequence of the S43 protein and the determined sequence of the CBPC 101 protein (6) indicates that we are actually dealing with a germ-line sequence and not with extensive somatic mutations of the IgG2a gene, although somatic mutations have been shown to occur in the VH gene which is linked to this C gene (11).…”

Section: Figmentioning

confidence: 99%

“…This gene has an additional GTG codon inserted after codon 424, thus conserving the reading frame and the length of the domain. The amino acid sequence of these proteins in the CH3 regions also required the same insertion and deletion to maintain homology (6).…”

Section: Figmentioning

confidence: 99%

See 2 more Smart Citations

Multiple differences between the nucleic acid sequences of the IgG2aa and IgG2ab alleles of the mouse.

Schreier¹,

Bothwell²,

Mueller-Hill³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

To compare the structure of IgG2a alleles we have determined the complete DNA sequence of the constant region, coding sequence, and 3' untranslated region of a cDNA clone, pAB gamma 2a-1, which was derived from the C57BL/6 mouse strain (b allotype). This sequence was compared with the corresponding IgG2a DNA sequence of BALB/c origin (a allotype). The DNA sequences showed 10% differences, and the deduced protein sequences differed by about 15%. These differences were not evenly distributed: most differences were in the hinge region, the CH3 domain and the 3' untranslated region. It is evident that many alterations in the IgG2a alleles have occurred since the a and b haplotypes were separated--some of these changes were point mutations but some appear to have resulted from gene conversion of the IgG2ab allele by the IgG2bb allele.

show abstract

Presentation of igg2a antigens to class ii‐restricted t cells by stably transfected b lymphoma cells

Bikoff

Eckhardt

1989

Eur J Immunol

View full text Add to dashboard Cite

Here we describe a panel of BALB/c T cells specific for IgG2a of the b allotype in association with I-Ad. We used DNA-mediated gene transfer techniques to localize antigenic determinants recognized by responding T cells. Initially a truncated IgG2aa gene comprising a variable domain and the CH3 domain (not including the membrane exons) from the BALB/c IgG2aa heavy chain was introduced into myeloma cells. The V-CH3 protein was expressed at high levels under control of the Ig heavy chain enhancer. Secretion of the V-CH3 protein did not require assembly of H-H dimers or an association with light chains. To generate stably transfected B cell lines that would stimulate our class II-restricted T cells, we replaced most of the BALB/c IgG2aa CH3 exon with CH3 coding sequences from a C57BL/6 IgG2ab cDNA clone and introduced these constructs into Ia+ B lymphoma cells. The IgG2ab CH3-transfected B cells were recognized by BALB/c Igh-1b-specific T cell hybrids in the absence of exogenous antigen. Experiments using glutaraldehyde-fixed cells as stimulators indicate that presentation of the secreted form of V-IgG2ab CH3 requires processing. We found that a significant fraction of the endogenously synthesized V-IgG2ab CH3 protein was, however, present as already processed antigen.

show abstract

Multiple amino acid substitutions between murine gamma 2a heavy chain Fc regions of Ig1a and Ig1b allotypic forms.

Cited by 11 publications

References 21 publications

Isotypic and allotypic specificity of mouse rheumatoid factors.

Isotypic and allotypic specificity of mouse rheumatoid factors.

Multiple differences between the nucleic acid sequences of the IgG2aa and IgG2ab alleles of the mouse.

Presentation of igg2a antigens to class ii‐restricted t cells by stably transfected b lymphoma cells

Contact Info

Product

Resources

About