Multiple Antenna Complexes in Various Purple Photosynthetic Bacteria

Bissig, Iwan; Wagner-Huber, R.; Brunisholz, René A.; Zuber, Herbert

doi:10.1007/978-1-4757-0893-6_23

Cited by 7 publications

(4 citation statements)

References 27 publications

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“…Because of the unusual properties of LH complexes of aerobic phototrophic bacteria, it would be of interest to determine the amino acid sequences of these antenna polypeptides. It also would be interesting to determine if E. ramosum forms a variety of LHII complexes dependent on temperature, oxygen, and light conditions, as do the anaerobic phototrophic bacteria Rhodopseudomonas acidophila and R. palustris (12,42,54,166).…”

Section: Light-harvesting Systemsmentioning

confidence: 99%

Aerobic Anoxygenic Phototrophic Bacteria

Yurkov

Beatty

1998

Microbiol Mol Biol Rev

429

241

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SUMMARY The aerobic anoxygenic phototrophic bacteria are a relatively recently discovered bacterial group. Although taxonomically and phylogenetically heterogeneous, these bacteria share the following distinguishing features: the presence of bacteriochlorophyll a incorporated into reaction center and light-harvesting complexes, low levels of the photosynthetic unit in cells, an abundance of carotenoids, a strong inhibition by light of bacteriochlorophyll synthesis, and the inability to grow photosynthetically under anaerobic conditions. Aerobic anoxygenic phototrophic bacteria are classified in two marine (Erythrobacter and Roseobacter) and six freshwater (Acidiphilium, Erythromicrobium, Erythromonas, Porphyrobacter, Roseococcus, and Sandaracinobacter) genera, which phylogenetically belong to the α-1, α-3, and α-4 subclasses of the class Proteobacteria. Despite this phylogenetic information, the evolution and ancestry of their photosynthetic properties are unclear. We discuss several current proposals for the evolutionary origin of aerobic phototrophic bacteria. The closest phylogenetic relatives of aerobic phototrophic bacteria include facultatively anaerobic purple nonsulfur phototrophic bacteria. Since these two bacterial groups share many properties, yet have significant differences, we compare and contrast their physiology, with an emphasis on morphology and photosynthetic and other metabolic processes.

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Section: Light-harvesting Systemsmentioning

confidence: 99%

Aerobic Anoxygenic Phototrophic Bacteria

Yurkov

Beatty

1998

Microbiol Mol Biol Rev

429

241

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“…vinosum (Mechler and Oelze 1978). The LH2 from highlight grown cells typically shows absorption maxima at 800 and 850 nm, whereas the low-light LH2 is characterized by a dominant absorption band at 800 nm with a shoulder at 820 nm (Thornber and Sokoloff 1970;Bissig et al 1990). Composition of the two forms can also be altered by use of different sulfur compounds in the culture media (Hayashi and Morita 1980).…”

Section: Introductionmentioning

confidence: 97%

“…Composition of the two forms can also be altered by use of different sulfur compounds in the culture media (Hayashi and Morita 1980). From the LH2 complex, three a-polypeptides and four b-polypeptides have been isolated and sequenced (Bissig et al 1990). Recently, the genome sequence of Alc.…”

Section: Introductionmentioning

confidence: 99%

Gene sequencing and characterization of the light-harvesting complex 2 from thermophilic purple sulfur bacterium Thermochromatium tepidum

et al. 2011

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In this study, gene sequences coding for the light-harvesting (LH) 2 polypeptides from a thermophilic purple sulfur bacterium Thermochromatium tepidum are reported and characterization of the LH2 complex is described. Three sets of pucBA genes have been identified, and the gene products have been analyzed by electrophoresis and reversed-phase chromatography. The result shows that all of the genes are expressed but the distribution of the expression is not uniform. The gene products undergo post-translational modification, where two of the β-polypeptides appear to be N-terminally methylated. Absorption spectrum of the purified LH2 complex exhibits Q (y) transitions at 800 and 854 nm in dodecyl β-maltopyranoside solution, and the circular dichroism spectrum shows a "molischianum"-like characteristic. No spectral change was observed for the LH2 when the bacterium was cultured under different conditions of light intensity. In lauryl dimethylamine N-oxide (LDAO) solution, significant changes in the absorption spectrum were observed. The B850 peak decreased and blue-shifted with increasing the LDAO concentration, whereas the B800 intensity increased without change in the peak position. The spectral changes can be partially or almost completely reversed by addition of metal ions, and the divalent cations seem to be more effective. The results indicate that ionic interactions may exist between LH2, detergent molecules and metal ions. Possible mechanisms involved in the detergent- and cation-induced spectral changes are discussed.

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“…Sequence analysis and circular dichroism measurements of antenna complexes of purple-nonsulfur bacteria suggest that each LH1 or LH2 antenna polypeptide spans the membrane once in the form of an a-helix (Cogdell & Scheer, 1985;Brunisholz et al, 1986). The limited biochemical data on purple-sulfur organisms (members of the Chromatiaceae) indicate subunit homology at the level of function and of primary sequence (Bissig et al, 1990;Kerfeld et al, manuscripts submitted and in prep. ).…”

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confidence: 99%