Multiple Antibiotic-resistant Escherichia coli in Ready-to-eat Foods from Food Outlets in Ekiti State University and Its Environ

Oje, Opeyemi James; David, Oluwole Moses; Adeosun, Omolade Mary; Adebayo, A. A.; Famurewa, O.

doi:10.9734/bmrj/2016/23477

Cited by 5 publications

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“…Another study reported that E. coli isolates were resistant to tetracycline (82.4%), which is similar to this study Oje et al [48]. However, another study found 100% of E. coli were resistant to ampicillin, amoxycillin, and tetracycline and 50% of E. coli were sensitive to gentamicin [40].…”

Section: Discussionsupporting

confidence: 86%

Isolation, identification, and antibiogram studies of Escherichia coli from ready-to-eat foods in Mymensingh, Bangladesh

et al. 2022

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Background and Aim: Ready-to-eat (RTE) foods are widely used at home, restaurants, and during festivals in Bangladesh. So it is very important to investigate possible microbial contamination in RTE foods. Therefore, this study aimed to determine the total coliform count (TCC), isolate, identify, and characterize the Escherichia coli in RTE foods. The antimicrobial sensitivity of E. coli obtained from RTE foods was also performed using 12 commonly used antibiotics. Materials and Methods: A total of 100 RTE food samples were collected aseptically and comprised of ten samples each: Burger, pizza, sandwich, chicken roll, chicken meat loaf, chicken fry, salad vegetable, ice-cream, yogurt, and milkshake sold in Mymensingh city. Samples were inoculated onto Eosin methylene blue agar and incubated at 37°C for 24 h. Isolation and identification of bacteria were performed based on cultural, staining, and biochemical properties, followed by a polymerase chain reaction. Results: The TCC in Chicken meat loaf, burger, pizza, sandwich, salad vegetable ice-cream, and yogurt samples were 3.57 ± 0.96, 3.69 ± 0.08, 3.50 ± 0.60, 2.60 ± 0.20, 4.09 ± 0.29, 4.44 ± 0.25, and 3.14 ± 0.30 mean log colony-forming units ± standard deviation/mL, respectively. The study found a higher prevalence of E. coli in RTE salad vegetable products than in RTE meat and milk products. Forty percent of the mixed vegetable salad samples showed positive results for E. coli. Whereas E. coli prevalence in RTE meat and milk products was 20% and 16.7%, respectively. All the 21 isolates were subjected to antibiotic susceptibility test against 12 different antibiotics. It was observed that 46.1% were susceptible, 16.6% were intermediate, 46.1% were resistant, and 47.6% were multidrug-resistant (MDR) among seven different antibiotic classes. E. coli isolates were resistant to cephalexin, ceftazidime, oxytetracycline, and ampicillin and sensitive to gentamycin, followed by kanamycin, ceftriaxone, colistin, and enrofloxacin.. Conclusion: The study revealed that RTE foods are a serious issue from a public health point of view. To achieve a safer level of E. coli in RTE foods sold for human consumption, public food outlets must improve hygienic and good production procedures. Moreover, MDR E. coli in these foods pose serious public health threats.

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Section: Discussionsupporting

confidence: 86%

Isolation, identification, and antibiogram studies of Escherichia coli from ready-to-eat foods in Mymensingh, Bangladesh

et al. 2022

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“…3 The responsibilities of good hygienic practices at home have therefore been shifted to food vendors even though some hardly observe such practices. 4,5 In most developing countries where unemployment rates are high, food vending outlets serve as a reliable source of food security to low-income earners and also act as a major form of generating income and employment. 6,7 Despite the numerous advantages associated with the sales of cooked food, contaminated food has been reported to present serious safety and health concerns to consumers and food handlers basically due to their diversity, inadequate food safety knowledge and practices, insufficient basic hygiene, and lack of public awareness.…”

Section: Introductionmentioning

confidence: 99%

Carriage of carbapenemase genes among multidrug resistant Escherichia coli isolates from contact surfaces in food vending outlets within Nasarawa State, Nigeria

Nfongeh

Dalhat²,

Kabido³

et al. 2022

IJMMTD

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Contamination of food contact surfaces by increases the risk of food-borne diseases through cross-contamination which becomes more complicated with the development of multidrug resistance by the pathogen. This study was aimed at investigating multidrug resistance and carriage of carbapenemase genes among isolates from food contact surfaces in Nasarawa State, Nigeria. : A total of 924 swab samples (522 from Lafia and 402 from Nasarawa Eggon) were collected from various food contact surfaces in food vending outlets and screened for multidrug resistance and carbapenemase genes in isolates using standard culture, modified Kirby-Bauer disk diffusion, PCR amplification and agarose gel electrophoresis techniques. Data obtained were statistically analyzed and p-value set at 0.05 confidence level. Highest overall contamination prevalence of 43.33 % was obtained from hawkers’ outlets while table top surfaces had 35.43% with highest risk (odd ratio) of 1.94. Eateries and Hotels had no contamination. The prevalence values were significantly different (p<0.05) among the food contact surfaces, vending outlets and the two communities. Isolates from street vendors obtained highest resistance to OFX, REF, STR, CEP, NAL, SEP, AMP antibiotics group with MDRI of 0.7. The bla, bla and bla carbapenemase genes were harbored by representative isolates. The presence of multidrug resistant with carbapenemase genes from food contact surfaces in vending outlets serves as a public health challenge and the need for personal hygiene and strict adherence to antibiotics protocols by food vendors is highly encouraged.

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Plant growth promoting potentials of novel phosphate-solubilizing bacteria isolated from rumen content of White Fulani cattle, indigenous to Nigeria

et al. 2022

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Multiple Antibiotic-resistant Escherichia coli in Ready-to-eat Foods from Food Outlets in Ekiti State University and Its Environ

Cited by 5 publications

References 0 publications

Isolation, identification, and antibiogram studies of Escherichia coli from ready-to-eat foods in Mymensingh, Bangladesh

Isolation, identification, and antibiogram studies of Escherichia coli from ready-to-eat foods in Mymensingh, Bangladesh

Carriage of carbapenemase genes among multidrug resistant Escherichia coli isolates from contact surfaces in food vending outlets within Nasarawa State, Nigeria

Plant growth promoting potentials of novel phosphate-solubilizing bacteria isolated from rumen content of White Fulani cattle, indigenous to Nigeria

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